About: Field‐Deployable Reverse Transcription‐Insulated Isothermal PCR (RT‐iiPCR) Assay for Rapid and Sensitive Detection of Foot‐and‐Mouth Disease Virus

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About: Field‐Deployable Reverse Transcription‐Insulated Isothermal PCR (RT‐iiPCR) Assay for Rapid and Sensitive Detection of Foot‐and‐Mouth Disease Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Field‐Deployable Reverse Transcription‐Insulated Isothermal PCR (RT‐iiPCR) Assay for Rapid and Sensitive Detection of Foot‐and‐Mouth Disease Virus
Creator	Fisher, M Nfon, C Ambagala, A Lung, O Canadian, A Furukawa-Stoffer, T Goolia, M Ortega Polo, R
Source	Medline; PMC
abstract	Foot‐and‐mouth disease (FMD) is a highly contagious viral disease of cloven‐hoofed animals, which can decimate the livestock industry and economy of countries previously free of this disease. Rapid detection of foot‐and‐mouth disease virus (FMDV) is critical to containing an FMD outbreak. Availability of a rapid, highly sensitive and specific, yet simple and field‐deployable assay would support local decision‐making during an FMDV outbreak. Here we report validation of a novel reverse transcription‐insulated isothermal PCR (RT‐iiPCR) assay that can be performed on a commercially available, compact and portable POCKIT (™) analyser that automatically analyses data and displays ‘+’ or ‘−’ results. The FMDV RT‐iiPCR assay targets the 3D region of the FMDV genome and was capable of detecting 9 copies of in vitro‐transcribed RNA standard with 95% confidence. It accurately identified 63 FMDV strains belonging to all seven serotypes and showed no cross‐reactivity with viruses causing similar clinical diseases in cloven‐hoofed animals. The assay was able to identify FMDV RNA in multiple sample types including oral, nasal and lesion swabs, epithelial tissue suspensions, vesicular and oral fluid samples, even before the appearance of clinical signs. Clinical sensitivity of the assay was comparable or slightly higher than the laboratory‐based real‐time RT‐PCR assay in use. The assay was able to detect FMDV RNA in vesicular fluid samples without nucleic acid extraction. For RNA extraction from more complex sample types, a commercially available taco(™) mini transportable magnetic bead‐based, automated extraction system was used. This assay provides a potentially useful field‐deployable diagnostic tool for rapid detection of FMDV in an outbreak in FMD‐free countries or for routine diagnostics in endemic countries with less structured laboratory systems.
has issue date	2016-09-03(xsd:dateTime)
bibo:doi	10.1111/tbed.12554
bibo:pmid	27589902
has license	no-cc
sha1sum (hex)	11c27b63c1686962a77a3eb22679c014212bff06
schema:url	https://doi.org/10.1111/tbed.12554
resource representing a document's title	Field‐Deployable Reverse Transcription‐Insulated Isothermal PCR (RT‐iiPCR) Assay for Rapid and Sensitive Detection of Foot‐and‐Mouth Disease Virus
has PubMed Central identifier	PMC7169878
has PubMed identifier	27589902
schema:publication	Transbound Emerg Dis
resource representing a document's body	covid:11c27b63c1686962a77a3eb22679c014212bff06#body_text
is schema:about of	named entity 'portable' named entity 'critical' named entity 'viruses' named entity 'outbreak' named entity 'complex' named entity 'disease' named entity 'strains' named entity 'sensitive' named entity 'Reverse' named entity 'PORTABLE' named entity 'COMPACT' named entity 'SYSTEM' named entity 'ABLE TO' named entity 'SYSTEMS' named entity 'CLINICAL SIGNS' named entity 'oral' named entity 'systems' named entity 'detecting' named entity 'Availability' named entity 'industry' named entity 'RNA' named entity 'thermocycling' named entity 'specific' named entity 'fluid' named entity 'support' named entity 'multiple' named entity 'transportable' named entity 'clinical signs' named entity 'Rapid' named entity 'foot-and-mouth disease virus' named entity 'vesicular' named entity 'RNA' named entity 'viral disease' named entity 'magnetic bead-based' named entity 'reverse transcription' named entity 'virus' named entity 'diagnostic tool' named entity 'genome' named entity 'sample' named entity 'vesicles' named entity 'Life Technologies' named entity 'virus' named entity 'supernatant' named entity 'pig' named entity 'sheep' named entity 'nucleic acid' named entity 'Zimbabwe' named entity 'clinical signs' named entity 'virus' named entity 'cell culture' named entity 'CHA' named entity 'veterinarians' named entity 'FMDV' named entity 'Lung' named entity 'car battery' named entity 'nucleic acid' named entity 'Epithelial tissues' named entity 'Suffolk' named entity 'cell culture' named entity 'gene' named entity 'pig' named entity 'vesicular stomatitis' named entity 'pathogens' named entity 'cattle' named entity '1.2' named entity 'University of Calgary' named entity 'sheep' named entity 'FMDV' named entity 'LED screen' named entity 'swine vesicular disease' named entity 'vesicular stomatitis' named entity 'phosphate-buffered saline' named entity 'pig' named entity 'PCR'

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