About: A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges

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About: A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges
Creator	Ding, Chan Liao, Ying Meng, Chunchun Qiu, Xusheng Song, Cuiping Sun, Yingjie Tan, Lei Liu, Weiwei Shao, Huabin Shi, Yonghong Wen, Guoyuan Yuan, Yanmei
Source	Medline; PMC
abstract	Infectious bronchitis (IB) and Newcastle disease (ND) are two major infectious diseases that are a threat to the domestic poultry industry. In this study, we successfully generated a recombinant LaSota candidate vaccine strain, rNDV-IBV-T/B, which expresses a short, synthetic, previously identified IBV S1 multi-epitope cassette using the reverse genetic system. The recombinant virus was propagated in nine-day-old embryonated chicken eggs for 20 passages and genetic stability was confirmed by whole genome DNA sequencing. The recombinant virus had a hemagglutination (HA) titer of 2(10), mean death time (MDT) of 118 hours, and intracerebral pathogenicity index (ICPI) of 0.05. None of these were significantly different from the parental Newcastle disease virus (NDV) LaSota strain (p > 0.05). Vaccination of white leghorn chickens at one day of age with 10(6) EID(50) rNDV-IBV-T/B provided 90% protection against virulent IBV M41 challenge at three weeks of age, which was significantly higher than the protection of the control group vaccinated with phosphate-buffered saline (PBS) (p < 0.05). The ciliostasis scores of rNDV-IBV-T/B-vaccinated and LaSota-vaccinated groups were 4.2 and 37.6, respectively, which indicated that rNDV-IBV-T/B vaccination reduced the pathogenicity of IBV toward the trachea. Furthermore, real-time RT-PCR assay showed that the rNDV-IBV-T/B vaccination resulted in low levels of viral load (647.80 ± 49.65 RNA copies) in the trachea four days post-challenge, which is significantly lower than groups vaccinated with PBS (8591.25 ± 311.10 RNA copies) or LaSota (7742.60 ± 298.50 RNA copies) (p < 0.05). Meanwhile, the same dose of rNDV-IBV-T/B vaccination provided complete protection against velogenic NDV F48E9 challenge. These results demonstrate that the rNDV-IBV-T/B strain is a promising vaccine candidate to control both IB and ND simultaneously. Furthermore, epitope-based live vector vaccines provide an alternative strategy for the development of cost-effective and, broadly, cross-protective vaccines.
has issue date	2019-11-01(xsd:dateTime)
bibo:doi	10.3390/vaccines7040170
bibo:pmid	31683905
has license	cc-by
sha1sum (hex)	111e82fd55d8b42cd523cf365b53dfcdaa2165d3
schema:url	https://doi.org/10.3390/vaccines7040170
resource representing a document's title	A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges
has PubMed Central identifier	PMC6963182
has PubMed identifier	31683905
schema:publication	Vaccines (Basel)
resource representing a document's body	covid:111e82fd55d8b42cd523cf365b53dfcdaa2165d3#body_text
is schema:about of	named entity 'assay' named entity 'IBV' named entity 'titer' named entity 'NDV' named entity 'RNA' named entity 'scores' named entity 'vaccinated' named entity 'Vaccine' named entity 'NDV' covid:arg/111e82fd55d8b42cd523cf365b53dfcdaa2165d3 named entity 'chicken' named entity 'reverse' named entity 'None' named entity 'PBS' named entity 'short' named entity 'RNA' named entity 'RNA' named entity 'genome' named entity 'copies' named entity 'PBS' named entity 'vaccination' named entity 'reduced' named entity 'synthetic' named entity 'poultry industry' named entity 'candidate' named entity 'passages' named entity 'demonstrate' named entity 'confirmed' named entity 'Multiple' named entity '4.2' named entity 'phosphate-buffered saline' named entity 'cross-protective' named entity 'infectious diseases' named entity 'vector' named entity 'domestic poultry' named entity 'DNA sequencing' named entity 'vaccination' named entity 'vaccine' named entity 'vaccines' named entity 'real-time RT-PCR' named entity 'control group' named entity 'Recombinant' named entity 'IBV' named entity 'Infectious Bronchitis Virus' named entity 'parental' named entity 'propagated' named entity 'Thermo Fisher Scientific' named entity 'vector' named entity 'epitope' named entity 'recombinant virus' named entity 'IBV' named entity 'PBS' named entity 'PBS' named entity 'chicken' named entity 'cell-mediated immune responses' named entity 'protein' named entity 'recombinant virus' named entity 'Titer' named entity 'IBV' named entity 'chicken' named entity 'gene' named entity 'Western blot' named entity 'infection' named entity 'kDa' named entity 'IBV' named entity 'serum samples' named entity 'epitope' named entity 'Genbank' named entity 'IBV' named entity 'PCR'

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