About: Replication of MERS and SARS coronaviruses in bat cells offers insights to their ancestral origins

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About: Replication of MERS and SARS coronaviruses in bat cells offers insights to their ancestral origins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Replication of MERS and SARS coronaviruses in bat cells offers insights to their ancestral origins
Creator	Yuen, Kwok-Yung Chan, Kwok-Hung Wernery, Ulrich Zhu, Longchao Ahmed, Chan, Jasper Fan, Rachel Fung, Kok, Raven Lau, Susanna Li, Kenneth Luk, Hayes Wong, Emily Woo, C Syed, Shakeel
Source	BioRxiv; MedRxiv
abstract	Previous findings of Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses in bats, and the ability of Tylonycteris-BatCoV HKU4 spike protein to utilize MERS-CoV receptor, human dipeptidyl peptidase 4 hDPP4, suggest a bat ancestral origin of MERS-CoV. We developed 12 primary bat cell lines from seven bat species, including Tylonycteris pachypus, Pipistrellus abramus and Rhinolophus sinicus (hosts of Tylonycteris-BatCoV HKU4, Pipistrellus-BatCoV HKU5 and SARS-related-CoV respectively), and tested their susceptibilities to MERS-CoVs, SARS-CoV and human coronavirus 229E (HCoV-229E). Five cell lines, including P. abramus and R. sinicus but not T. pachypus cells, were susceptible to human MERS-CoV EMC/2012. However, three tested camel MERS-CoV strains showed different infectivities, with only two strains capable of infecting three and one cell lines respectively. SARS-CoV can only replicate in R. sinicus cells, while HCoV-229E cannot replicate in any bat cells. Bat dipeptidyl peptidase 4 (DPP4) sequences were closely related to those of human and non-human primates but distinct from dromedary DPP4 sequence. Critical residues for binding to MERS-CoV spike protein were mostly conserved in bat DPP4. DPP4 was expressed in the five bat cells susceptible to MERS-CoV, with significantly higher mRNA expression levels than those in non-susceptible cells (P=0.0174), supporting that DPP4 expression is critical for MERS-CoV infection in bats. However, overexpression of T. pachypus DPP4 failed to confer MERS-CoV susceptibility in T. pachypus cells, suggesting other cellular factors in determining viral replication. The broad cellular tropism of MERS-CoV should prompt further exploration of host diversity of related viruses to identify its ancestral origin.
has issue date	2018-05-20(xsd:dateTime)
bibo:doi	10.1101/326538
has license	medrxiv
sha1sum (hex)	10abb3e977aa476a39bce4f1feda37c2e1250106
schema:url	https://doi.org/10.1101/326538
resource representing a document's title	Replication of MERS and SARS coronaviruses in bat cells offers insights to their ancestral origins
schema:publication	bioRxiv
resource representing a document's body	covid:10abb3e977aa476a39bce4f1feda37c2e1250106#body_text
is schema:about of	named entity 'infecting' named entity 'human' named entity 'origin' named entity 'EMC/2012' named entity 'host' named entity 'camel' named entity 'DPP4' named entity 'susceptible' named entity 'ALLOWED' named entity 'REPLICATION' named entity 'ORIGINS' named entity 'SUSCEPTIBILITY' named entity 'INCLUDING' named entity 'DIPEPTIDYL PEPTIDASE 4' named entity 'RHINOLOPHUS SINICUS' named entity 'CORONAVIRUSES' named entity 'MERS' named entity 'BAT' named entity 'EXPRESSED' named entity 'INFECTING' named entity 'CELL LINES' named entity 'EXPLORATION' named entity 'CELLULAR' named entity 'EXPRESSION' named entity 'PIPISTRELLUS ABRAMUS' named entity 'PIPISTRELLUS' named entity 'STRAINS' named entity 'SUSCEPTIBLE' named entity 'HCOV-229E' named entity 'ONE CELL' named entity 'PRIMATES' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'RIGHTS' named entity 'MERS' named entity 'PERMISSION' named entity 'SARS' named entity 'PACHYPUS' named entity 'NON-SUSCEPTIBLE' named entity 'BATS' named entity 'SUGGEST' named entity 'INSIGHTS' named entity 'DPP4' named entity 'HUMAN' named entity 'HOST' named entity 'CELLS' named entity 'THEIR' named entity 'SARS' named entity 'FAILED' named entity 'CRITICAL' named entity '229E' named entity 'NON-' named entity 'SEVEN' named entity 'ABILITY' named entity 'SUSCEPTIBILITIES' named entity 'CAMEL' named entity 'CELLS' named entity 'TYLONYCTERIS PACHYPUS' named entity 'OVEREXPRESSION' named entity 'BUT' named entity 'THEIR' named entity 'TYLONYCTERIS' named entity 'PREVIOUS FINDINGS' named entity 'RECEPTOR' named entity 'MRNA' named entity 'TO IDENTIFY' named entity 'BINDING' named entity 'ITS' named entity 'TESTED' named entity 'EMC' named entity 'DIVERSITY'

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