About: Next-Generation Sequencing Reveals HIV-1-Mediated Suppression of T Cell Activation and RNA Processing and Regulation of Noncoding RNA Expression in a CD4(+) T Cell Line

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About: Next-Generation Sequencing Reveals HIV-1-Mediated Suppression of T Cell Activation and RNA Processing and Regulation of Noncoding RNA Expression in a CD4(+) T Cell Line Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Next-Generation Sequencing Reveals HIV-1-Mediated Suppression of T Cell Activation and RNA Processing and Regulation of Noncoding RNA Expression in a CD4(+) T Cell Line
Creator	Peng, Xinxia Sova, Pavel Weiss, Jeffrey Chang, Stewart Katze, Michael Law, G Palermo, Robert
Source	PMC
abstract	Next-generation sequencing (NGS) enables the highly sensitive measurement of whole transcriptomes. We report the first application to our knowledge of this technology to the analysis of RNA from a CD4(+) T cell line infected with intact HIV. We sequenced the total mRNA from infected cells and detected differences in the expression of both host and viral mRNA. Viral reads represented a large portion of the total mapped sequencing reads: approximately 20% at 12 h postinfection (hpi) and 40% at 24 hpi. We also detected a small but significant suppression of T cell activation-related genes at 12 hpi. This suppression persisted and expanded by 24 hpi, providing new possible markers of virus-induced T cell cytopathology. By 24 hpi, the expression of over 50% of detectable host loci was also altered, indicating widespread alteration of host processes, including RNA processing, splicing, and transport to an extent not previously reported. In addition, next-generation sequencing provided insights into alternative viral RNA splice events and the expression of noncoding RNAs, including microRNA host genes.
has issue date	2011-09-20(xsd:dateTime)
bibo:doi	10.1128/mbio.00134-11
bibo:pmid	21933919
has license	cc-by-nc-sa
sha1sum (hex)	102e8dd2d010be4e7f39f8e2d0c84190ee87d9e2
schema:url	https://doi.org/10.1128/mbio.00134-11
resource representing a document's title	Next-Generation Sequencing Reveals HIV-1-Mediated Suppression of T Cell Activation and RNA Processing and Regulation of Noncoding RNA Expression in a CD4(+) T Cell Line
has PubMed Central identifier	PMC3175625
has PubMed identifier	21933919
schema:publication	mBio
resource representing a document's body	covid:102e8dd2d010be4e7f39f8e2d0c84190ee87d9e2#body_text
is schema:about of	named entity 'REGULATION' named entity 'CELL LINE' named entity 'T CELL' named entity 'NEXT-GENERATION SEQUENCING' covid:arg/102e8dd2d010be4e7f39f8e2d0c84190ee87d9e2 named entity 'CD4' named entity 'EXPANDED ' named entity 'ADDITION' named entity 'SPLICE' named entity 'ALTERATION' named entity 'PROCESSES' named entity 'TRANSPORT' named entity 'LARGE' named entity 'INCLUDING' named entity 'VIRUS' named entity 'ENABLES' named entity 'MAPPED' named entity 'VIRAL' named entity 'TECHNOLOGY' named entity 'EVENTS' named entity 'ALTERNATIVE' named entity 'CYTOPATHOLOGY' named entity 'ALTERED' named entity 'SUPPRESSION' named entity 'VIRAL RNA' named entity 'DETECTED' named entity 'RNA PROCESSING' named entity 'VIRAL MRNA' named entity 'BUT' named entity 'REPORTED' named entity 'PROVIDING' named entity 'MRNA' named entity '20%' named entity 'SUPPRESSION OF T CELL ACTIVATION' named entity 'NEXT-GENERATION SEQUENCING' named entity 'MEDIATED' named entity 'SUPPRESSION OF T CELL ACTIVATION' named entity 'HIV-1' named entity 'NONCODING RNA' named entity 'CELLS' named entity '40%' named entity 'READS' named entity 'INFECTED' named entity 'RNA PROCESSING' named entity 'RNA EXPRESSION' named entity 'ANALYSIS' named entity 'RELATED' named entity 'KNOWLEDGE' named entity 'REPORT' named entity 'HIGHLY SENSITIVE' named entity 'NEW' named entity 'POSSIBLE' named entity 'EXPRESSION' named entity 'HIV' named entity 'PROVIDED' named entity 'PORTION' named entity 'APPROXIMATELY' named entity 'LOCI' named entity 'WIDESPREAD' named entity 'CELL LINE' named entity 'INDICATING' named entity 'EXTENT' named entity 'REVEALS' named entity 'INDUCED' named entity 'CD4' named entity 'TOTAL' named entity 'MEASUREMENT' named entity 'HPI' named entity 'SMALL' named entity 'MARKERS' named entity 'SEQUENCING READS' named entity 'SPLICING' named entity 'DETECTABLE' named entity 'SIGNIFICANT' named entity 'INTACT'

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