About: Functional peptide microarrays for specific and sensitive antibody diagnostics

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Functional peptide microarrays for specific and sensitive antibody diagnostics
Creator	Ehrentreich-Förster, Eva Andresen, Heiko Grötzinger, Carsten Zarse, Kim Bier, Frank Kreuzer, Oliver
Source	Medline; PMC
abstract	Peptide microarrays displaying biologically active small synthetic peptides in a high‐density format provide an attractive technology to probe complex samples for the presence and/or function of protein analytes. We present a new approach for manufacturing functional peptide microarrays for molecular immune diagnostics. Our method relies on the efficiency of site‐specific solution‐phase coupling of biotinylated synthetic peptides to NeutrAvidin (NA) and localized microdispensing of peptide‐NA‐complexes onto activated glass surfaces. Antibodies are captured in a sandwich manner between surface immobilized peptide probes and fluorescence‐labeled secondary antibodies. Our work includes a total of 54 peptides derived from immunodominant linear epitopes of the T7 phage capsid protein, Herpes simplex virus glycoprotein D, c‐myc protein, and three domains of the Human coronavirus polymerase polyprotein and their cognate mAbs. By using spacer molecules of different type and length for NA‐mediated peptide presentation, we show that the incorporation of a minimum spacer length is imperative for antibody binding, whereas the peptide immobilization direction has only secondary importance for antibody affinity and binding. We further demonstrate that the peptide array is capable of detecting low‐picomolar concentrations of mAbs in buffered solutions and diluted human serum with high specificity.
has issue date	2006-02-03(xsd:dateTime)
bibo:doi	10.1002/pmic.200500343
bibo:pmid	16456884
has license	no-cc
sha1sum (hex)	0f1fccb7234ada04fa3f2dd8dc334dd897bbb5d9
schema:url	https://doi.org/10.1002/pmic.200500343
resource representing a document's title	Functional peptide microarrays for specific and sensitive antibody diagnostics
has PubMed Central identifier	PMC7167710
has PubMed identifier	16456884
schema:publication	Proteomics
resource representing a document's body	covid:0f1fccb7234ada04fa3f2dd8dc334dd897bbb5d9#body_text
is schema:about of	named entity 'surface' named entity 'analytes' named entity 'SOLUTION' named entity 'INCLUDES' named entity 'ANTIBODIES' named entity 'DIAGNOSTICS' named entity 'ANTIBODY' named entity 'PRESENTATION' named entity 'ACTIVE' named entity 'derived' named entity 'mAbs' named entity 'surfaces' named entity 'epitopes' named entity 'localized' named entity 'Peptide' named entity 'Antibodies' named entity 'domains' named entity 'peptide' named entity 'peptide' named entity 'incorporation' named entity 'Herpes simplex virus' named entity 'peptide' named entity 'protein' named entity 'serum' named entity 'fluorescence' named entity 'protein' named entity 'epitopes' named entity 'presence' named entity 'peptide' named entity 'dissociation constant' named entity 'protein' named entity 'resin' named entity 'peptide microarrays' named entity 'capsid protein' named entity 'Fmoc' named entity 'antibodies' named entity 'potassium' named entity 'recombinant proteins' named entity 'Germany' named entity 'protein-protein interactions' named entity 'fluorescence' named entity 'RFU' named entity 'PBS' named entity 'amine' named entity 'hydrogen' named entity 'Affymetrix' named entity 'hydrogen' named entity 'peptide chemistry' named entity 'immune responses' named entity 'skim milk' named entity 'peptides' named entity 'antibody repertoire' named entity 'epitope' named entity 'microarrays' named entity 'biotin' named entity 'peptide' named entity 'Cy5' named entity 'C-terminal' named entity 'piperidine' named entity 'sodium chloride' named entity 'peptide' named entity 'mAb' named entity 'mAb' named entity 'polyclonal' named entity 'carrier protein' named entity 'peptide' named entity 'binding sites' named entity 'antibody' named entity 'peptide' named entity 'PBS' named entity 'Peptides' named entity 'kDa' named entity 'Pol' named entity 'PNAC' named entity 'HPLC' named entity 'antibody'

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