About: A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

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About: A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
Creator	Belák, Sándor Liu, Lihong Blomberg, Jonas Eriksson, Ronnie Kiss, István Merza, Malik Nordengrahn, Ann Xia, Hongyan
Source	Elsevier; Medline; PMC
abstract	This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes.
has issue date	2010-04-18(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.04.009
bibo:pmid	20403384
has license	no-cc
sha1sum (hex)	0e98b5502ae17b1c4308d4794a0b3d3d99530b84
schema:url	https://doi.org/10.1016/j.jviromet.2010.04.009
resource representing a document's title	A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
has PubMed Central identifier	PMC7119709
has PubMed identifier	20403384
schema:publication	J Virol Methods
resource representing a document's body	covid:0e98b5502ae17b1c4308d4794a0b3d3d99530b84#body_text
is schema:about of	named entity 'ANTIBODIES' named entity 'sensitivity' named entity 'bead' named entity 'This' named entity 'viral' named entity 'The' named entity 'serum' named entity 'bovine' named entity 'diarrhoea' named entity 'NEW' named entity 'SPECIFIC' named entity 'PERFORMANCE' named entity 'SAMPLES' named entity 'CONVENTIONAL' named entity 'CUT-OFF VALUE' named entity 'TESTING' named entity 'USED' named entity 'NEGATIVE' named entity 'VARIABILITY' named entity 'IMMUNOASSAY' named entity 'VIRUS' named entity 'BASED' named entity 'HIGH-THROUGHPUT SCREENING' named entity 'PROVIDES' named entity 'ASSAY' named entity 'SENSITIVE' named entity 'RAPID' named entity 'DETECTION' named entity 'VIRUS' named entity 'MICROSPHERE' named entity 'CONTROL' named entity 'BLOCKING' named entity 'SERUM' named entity 'VIRAL DIARRHOEA' named entity 'EVALUATED' named entity 'SPECIFICITY' named entity 'VIRAL DIARRHOEA' named entity 'INTRA' named entity 'BOVINE' named entity 'BASED' named entity 'SYSTEMS' named entity 'HIGHLY SENSITIVE' named entity 'BVD' named entity 'STUDY' named entity 'INTER-' named entity 'SENSITIVITY' named entity 'NOVEL' named entity 'ANTIBODIES' named entity 'RELATIVE TO' named entity 'LESS THAN' named entity '509' named entity 'DETECTION' named entity 'IMMUNOASSAY' named entity 'DIAGNOSTIC' named entity 'BEAD' named entity 'ALTERNATIVE' named entity 'ELISA' named entity '6.6' named entity 'MICROSPHERE' named entity 'BOVINE' named entity 'TOTAL' covid:arg/0e98b5502ae17b1c4308d4794a0b3d3d99530b84 named entity 'bovine' named entity 'BVD' named entity 'assay' named entity 'testing' named entity 'conjugations' named entity 'microsphere' named entity 'BVDV' named entity 'virus' named entity 'bovine viral diarrhoea' named entity 'bovine' named entity 'antibody' named entity 'Luminex'

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