About: Antigenicity Analysis of Different Regions of the Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Antigenicity Analysis of Different Regions of the Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein
Creator	Li, Bei Yang, Ruifu Han, Yanping Zhai, Junhui Wang, Jin Chen, Zeliang Du, Zongmin Guo, Zhaobiao Jiang, Lingxiao Wang, Hongxia Zhou, Dongsheng Qiu, Maofeng Song, Yajun Pei, Decui
Source	Medline; PMC
abstract	Background: The widespread threat of severe acute respiratory syndrome (SARS) to human health has made urgent the development of fast and accurate analytical methods for its early diagnosis and a safe and efficient antiviral vaccine for preventive use. For this purpose, we investigated the antigenicity of different regions of the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein. Methods: The cDNA for full-length N protein and its various regions from the SARS-CoV was cloned and expressed in Escherichia coli. After purification, all of the protein fragments were printed on glass slides to fabricate a protein microarray and then probed with the sera from SARS patients to determine the reactivity of these protein fragments. Results: The full-length protein and two other fragments reacted with all 52 sera tested. Four important regions with possible epitopes were identified and named as EP1 (amino acids 51–71), EP2 (134–208), EP3 (249–273), and EP4 (349–422), respectively. EP2 and EP4 possessed linear epitopes, whereas EP1 and EP2 were able to form conformational epitopes that could react with most (>80%) of the tested sera. EP3 and EP4 also formed conformational epitopes, and antibodies against these epitopes existed in all 52 of the sera tested. Conclusion: The N protein is a highly immunogenic protein of the SARS-CoV. Conformational epitopes are important for this protein, and antigenicity of the COOH terminus is higher than that of the NH(2) terminus. The N protein is a potential diagnostic antigen and vaccine candidate for SARS-CoV.
has issue date	2004-06-01(xsd:dateTime)
bibo:doi	10.1373/clinchem.2004.031096
bibo:pmid	15054081
has license	no-cc
sha1sum (hex)	0e56e75c7d5b2cb420cc72e8d05c3ef118b70d59
schema:url	https://doi.org/10.1373/clinchem.2004.031096
resource representing a document's title	Antigenicity Analysis of Different Regions of the Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein
has PubMed Central identifier	PMC7108132
has PubMed identifier	15054081
schema:publication	Clin Chem
resource representing a document's body	covid:0e56e75c7d5b2cb420cc72e8d05c3ef118b70d59#body_text
is schema:about of	named entity 'preventive' named entity 'investigated' named entity 'Severe Acute Respiratory Syndrome Coronavirus' named entity 'FAST' named entity 'DEVELOPMENT' named entity 'ITS' named entity 'THREAT' named entity 'SAFE' named entity 'DIFFERENT' named entity 'ANTIGENICITY' named entity 'USE' named entity 'INVESTIGATED' named entity 'ANALYTICAL METHODS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'URGENT' named entity 'REGIONS' named entity 'DIFFERENT' named entity 'ANALYSIS' named entity 'NUCLEOCAPSID PROTEIN' named entity 'ANTIGENICITY' named entity 'VACCINE' named entity 'PROTEIN' named entity 'PURPOSE' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'EFFICIENT' named entity 'ANTIVIRAL' named entity 'REGIONS' named entity 'SARS CORONAVIRUS' named entity 'ACCURATE' named entity 'WIDESPREAD' named entity 'NUCLEOCAPSID' named entity 'EARLY DIAGNOSIS' named entity 'HUMAN HEALTH' named entity 'PREVENTIVE' covid:arg/0e56e75c7d5b2cb420cc72e8d05c3ef118b70d59 named entity 'regions' named entity 'vaccine' named entity 'SARS coronavirus' named entity 'Severe Acute Respiratory Syndrome Coronavirus' named entity 'Nucleocapsid Protein' named entity 'protein' named entity 'Guangdong Province' named entity 'enveloped virus' named entity 'RNA-binding' named entity 'microarray' named entity 'epitopes' named entity 'IgG' named entity 'COOH terminus' named entity 'nonstructural proteins' named entity 'serum' named entity 'reagent' named entity '1:200' named entity 'SARS' named entity 'genome' named entity 'RNA' named entity 'ethanol' named entity 'immunoblotting' named entity 'antibodies' named entity 'sodium dodecyl sulfate' named entity 'protein' named entity 'clones' named entity 'ELISAs' named entity 'antigen' named entity 'antigenicity' named entity 'antigenicity' named entity 'microarray' named entity 'amino acids' named entity 'gene function'

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