About: Pooled samples and eDNA-based detection can facilitate the “clean trade” of aquatic animals

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About: Pooled samples and eDNA-based detection can facilitate the “clean trade” of aquatic animals Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Pooled samples and eDNA-based detection can facilitate the “clean trade” of aquatic animals
Creator	Brunner, Jesse
Source	Medline; PMC
abstract	The regional and international trade of live animals facilitates the movement, spillover, and emergence of zoonotic and epizootic pathogens around the world. Detecting pathogens in trade is critical for preventing their continued movement and introduction, but screening a sufficient fraction to ensure rare infections are detected is simply infeasible for many taxa and settings because of the vast numbers of animals involved—hundreds of millions of live animals are imported into the U.S.A. alone every year. Batch processing pools of individual samples or using environmental DNA (eDNA)—the genetic material shed into an organism’s environment—collected from whole consignments of animals may substantially reduce the time and cost associated with pathogen surveillance. Both approaches, however, lack a framework with which to determine sampling requirements and interpret results. Here I present formulae for pooled individual samples (e.g,. swabs) and eDNA samples collected from finite populations and discuss key assumptions and considerations for their use with a focus on detecting Batrachochytrium salamandrivorans, an emerging pathogen that threatens global salamander diversity. While empirical validation is key, these formulae illustrate the potential for eDNA-based detection in particular to reduce sample sizes and help bring clean trade into reach for a greater number of taxa, places, and contexts.
has issue date	2020-06-24(xsd:dateTime)
bibo:doi	10.1038/s41598-020-66280-7
bibo:pmid	32581260
has license	cc-by
sha1sum (hex)	0e4e44706c6ce261887b51d06eccc967dfb1482e
schema:url	https://doi.org/10.1038/s41598-020-66280-7
resource representing a document's title	Pooled samples and eDNA-based detection can facilitate the “clean trade” of aquatic animals
has PubMed Central identifier	PMC7314758
has PubMed identifier	32581260
schema:publication	Sci Rep
resource representing a document's body	covid:0e4e44706c6ce261887b51d06eccc967dfb1482e#body_text
is schema:about of	named entity 'individual' named entity 'populations' named entity 'consignments' named entity 'live' named entity 'infections' named entity 'number' named entity 'fraction' named entity 'sampling' named entity 'sample sizes' named entity 'samples' named entity 'TIME' named entity 'PATHOGEN SURVEILLANCE' named entity 'SALAMANDER' named entity 'THESE' named entity 'SETTINGS' named entity 'PREVENTING' named entity 'INTERNATIONAL TRADE' named entity 'EMERGING PATHOGEN' covid:arg/0e4e44706c6ce261887b51d06eccc967dfb1482e named entity 'Batrachochytrium salamandrivorans' named entity 'samples' named entity 'zoonotic' named entity 'Both' named entity 'environmental DnA' named entity 'contexts' named entity 'eDNA' named entity 'organism' named entity 'empirical validation' named entity 'live animals' named entity 'genetic material' named entity 'zoonotic' named entity 'Ranavirus' named entity 'sampling with replacement' named entity 'nature.com' named entity 'live animal' named entity 'eDNA' named entity 'Megalocytivirus' named entity 'dose-response relationship' named entity 'analyte' named entity 'eDNA' named entity 'antibodies' named entity 'eDNA' named entity 'ballast water' named entity 'amphibians' named entity 'Bsal' named entity 'PCR' named entity 'infection' named entity 'eDNA' named entity 'PCR' named entity 'Canada' named entity 'eDNA' named entity 'quarantine' named entity 'eDNA' named entity 'eDNA' named entity 'eDNA' named entity 'waste material' named entity 'analyte' named entity 'analyte' named entity 'Empirical validation' named entity 'nature.com' named entity 'live animal' named entity 'amphibians' named entity 'infection' named entity 'international trade' named entity 'eDNA' named entity 'syphilis' named entity 'environmental DNA' named entity 'infection status' named entity 'aquatic' named entity 'PCR' named entity 'African clawed frogs' named entity 'Baldock' named entity 'taxa' named entity 'amphibians' named entity 'amphibian' named entity 'eDNA' named entity 'eDNA' named entity 'eDNA' named entity 'antigens' named entity 'international trade' named entity 'pet trade'

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