About: Simultaneous Detection of CDC Category “A” DNA and RNA Bioterrorism Agents by Use of Multiplex PCR & RT-PCR Enzyme Hybridization Assays

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About: Simultaneous Detection of CDC Category “A” DNA and RNA Bioterrorism Agents by Use of Multiplex PCR & RT-PCR Enzyme Hybridization Assays Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simultaneous Detection of CDC Category “A” DNA and RNA Bioterrorism Agents by Use of Multiplex PCR & RT-PCR Enzyme Hybridization Assays
Creator	Fan, Jiang He, Jie Khanna, Marilyn Wang, Lihua Metallo, Jacob Bose, Michael Henrickson, Kelly Kraft, Andrea Dyke, Meredith
Source	Medline; PMC
abstract	Assays to simultaneously detect multiple potential agents of bioterrorism are limited. Two multiplex PCR and RT-PCR enzyme hybridization assays (mPCR-EHA, mRT-PCR-EHA) were developed to simultaneously detect many of the CDC category “A” bioterrorism agents. The “Bio T” DNA assay was developed to detect: Variola major (VM), Bacillus anthracis (BA), Yersinia pestis (YP), Francisella tularensis (FT) and Varicella zoster virus (VZV). The “Bio T” RNA assay (mRT-PCR-EHA) was developed to detect: Ebola virus (Ebola), Lassa fever virus (Lassa), Rift Valley fever (RVF), Hantavirus Sin Nombre species (HSN) and dengue virus (serotypes 1–4). Sensitivity and specificity of the 2 assays were tested by using genomic DNA, recombinant plasmid positive controls, RNA transcripts controls, surrogate (spiked) clinical samples and common respiratory pathogens. The analytical sensitivity (limit of detection (LOD)) of the DNA asssay for genomic DNA was 1×10(0)∼1×10(2) copies/mL for BA, FT and YP. The LOD for VZV whole organism was 1×10(−2) TCID(50)/mL. The LOD for recombinant controls ranged from 1×10(2)∼1×10(3)copies/mL for BA, FT, YP and VM. The RNA assay demonstrated LOD for RNA transcript controls of 1×10(4)∼1×10(6) copies/mL without extraction and 1×10(5)∼1×10(6) copies/mL with extraction for Ebola, RVF, Lassa and HSN. The LOD for dengue whole organisms was ∼1×10(−4) dilution for dengue 1 and 2, 1×10(4) LD(50)/mL and 1×10(2) LD(50)/mL for dengue 3 and 4. The LOD without extraction for recombinant plasmid DNA controls was ∼1×10(3) copies/mL (1.5 input copies/reaction) for Ebola, RVF, Lassa and HSN. No cross-reactivity of primers and probes used in both assays was detected with common respiratory pathogens or between targeted analytes. Clinical sensitivity was estimated using 264 surrogate clinical samples tested with the BioT DNA assay and 549 samples tested with the BioT RNA assay. The clinical specificity is 99.6% and 99.8% for BioT DNA assay and BioT RNA assay, respectively. The surrogate sensitivities of these two assays were 100% (95%CI 83–100) for FT, BA (pX02), YP, VM, VZV, dengue 2,3,4 and 95% (95%CI 75–100) for BA (pX01) and dengue 1 using spiked clinical specimens. The specificity of both BioT multiplex assays on spiked specimens was 100% (95% CI 99–100). Compared to other available assays (culture, serology, PCR, etc.) both the BioT DNA mPCR-EHA and BioT RNA mRT-PCR-EHA are rapid, sensitive and specific assays for detecting many category “A” Bioterrorism agents using a standard thermocycler.
has issue date	2009-10-20(xsd:dateTime)
bibo:doi	10.3390/v1030441
bibo:pmid	20224751
has license	cc-by
sha1sum (hex)	0ddcebebb542f8a0905a3cf82a59f404158d3de2
schema:url	https://doi.org/10.3390/v1030441
resource representing a document's title	Simultaneous Detection of CDC Category “A” DNA and RNA Bioterrorism Agents by Use of Multiplex PCR & RT-PCR Enzyme Hybridization Assays
has PubMed Central identifier	PMC2836126
has PubMed identifier	20224751
schema:publication	Viruses
resource representing a document's body	covid:0ddcebebb542f8a0905a3cf82a59f404158d3de2#body_text
is schema:about of	named entity 'RNA' named entity 'VZV' named entity 'USE OF' named entity '9710' named entity 'ENZYME' named entity 'HSN' named entity '22A' named entity 'LOD' named entity 'TESTED' named entity 'LASSA FEVER VIRUS' named entity 'PCR' named entity 'COMMON' named entity 'FRANCISELLA TULARENSIS' named entity 'CDC' named entity 'POTENTIAL' named entity 'RESPIRATORY PATHOGENS' named entity 'RECOMBINANT PLASMID' named entity 'HANTAVIRUS SIN NOMBRE' named entity 'SPECIES' named entity 'RT-PCR' named entity 'CATEGORY' named entity 'VARICELLA ZOSTER VIRUS' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'RT-PCR' named entity 'POSITIVE' named entity 'YERSINIA PESTIS' named entity 'BACILLUS ANTHRACIS' named entity 'TRANSCRIPTS' named entity 'USING' named entity 'DETECTION' named entity 'MULTIPLE' named entity 'HYBRIDIZATION' named entity 'RIFT VALLEY FEVER' named entity 'SIMULTANEOUSLY' named entity 'RNA ASSAY' named entity 'SIMULTANEOUS' named entity 'DETECTION' named entity 'BIOTERRORISM' named entity 'EBOLA VIRUS' named entity 'CATEGORY' named entity 'DNA' named entity 'DNA ASSAY' named entity 'DETECT' named entity 'SENSITIVITY' named entity 'MRT' named entity 'WHOLE ORGANISM' named entity 'CLINICAL' named entity 'TO DETECT' named entity 'VARIOLA MAJOR' named entity 'RECOMBINANT' named entity 'TCID' named entity 'MULTIPLEX PCR' named entity 'HYBRIDIZATION' named entity 'DILUTION' named entity 'RNA' named entity 'SURROGATE' named entity 'ENZYME' named entity 'EBOLA' named entity 'GENOMIC DNA' named entity 'INPUT' named entity 'PLASMID DNA' named entity 'LASSA' named entity 'LIMITED' named entity 'RNA ASSAY' named entity 'RNA TRANSCRIPT' named entity 'ORGANISMS' named entity 'DENGUE' named entity 'DEMONSTRATED' named entity 'ANALYTICAL' named entity 'DENGUE VIRUS' named entity 'MULTIPLEX PCR' named entity 'SAMPLES' named entity 'BIOTERRORISM'

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