About: Kinetics of the in vitro antibody response to transmissible gastroenteritis (TGE) virus from pig mesenteric lymph node cells, using the ELISASPOT and ELISA tests

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About: Kinetics of the in vitro antibody response to transmissible gastroenteritis (TGE) virus from pig mesenteric lymph node cells, using the ELISASPOT and ELISA tests Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Kinetics of the in vitro antibody response to transmissible gastroenteritis (TGE) virus from pig mesenteric lymph node cells, using the ELISASPOT and ELISA tests
Creator	Bernard, S Berthon, P Binns, R Salmon, H
Source	Elsevier; Medline; PMC
abstract	Abstract A method is described for in vitro studies of viral humoral immune responses in the pig. After oral immunization with transmissible gastroenteritis (TGE) coronavirus, antibody production from primed mesenteric lymph node cells was revealed by an in vitro boost with viral antigen. For the latter the leukocytes were co-cultured with UV-inactivated virus using a variety of different methods of antigenic stimulation. Enumeration of specific antibody-secreting cells (ASC) and titration of secreted anti-virus antibodies were performed with ELISASPOT (using 3-amino 9-ethyl carbazole as the peroxidase chromogen) and ELISA tests respectively, according to the Ig isotype. The results showed a close relationship between ASC numbers and secreted antibody titres. The best in vitro antibody synthesis was observed when the sensitized cells were maintained in contact with virus during the whole culture period. Antibody responses were defined by a kinetic profile characterized by a narrow peak, with a maximum occuring after 4 and 6 days of culture and with the IgA response appearing earlier than the IgG. This methodology, which analyses specific antibody responses at the cellular level, may permit studies on the mechanisms of Ig isotype regulation. Extended to leukocytes from other organs of the immune system, it may also constitute an in vitro model to study antibody responses expressed in different lymphoid tissues of the pig.
has issue date	1990-08-07(xsd:dateTime)
bibo:doi	10.1016/0022-1759(90)90188-2
bibo:pmid	2167914
has license	els-covid
sha1sum (hex)	0cf4d4a1e364402ae2242cd5a270b50d25a2dfca
schema:url	https://doi.org/10.1016/0022-1759%2890%2990188-2
resource representing a document's title	Kinetics of the in vitro antibody response to transmissible gastroenteritis (TGE) virus from pig mesenteric lymph node cells, using the ELISASPOT and ELISA tests
has PubMed Central identifier	PMC7130707
has PubMed identifier	2167914
schema:publication	Journal of Immunological Methods
resource representing a document's body	covid:0cf4d4a1e364402ae2242cd5a270b50d25a2dfca#body_text
is schema:about of	named entity 'Enumeration' named entity 'IgG' named entity 'amino' named entity 'cellular' named entity 'profile' named entity 'isotype' named entity 'TGE' named entity 'SPECIFIC ANTIBODY' named entity 'METHODS' named entity 'IN VITRO' named entity 'CORONAVIRUS' named entity 'IMMUNE RESPONSES' named entity 'LEUKOCYTES' named entity 'METHOD' named entity 'ANTIBODY PRODUCTION' named entity 'IGG' named entity 'BEST' named entity 'TITRATION' named entity 'SYNTHESIS' named entity 'CHROMOGEN' named entity 'USING' named entity 'IN CONTACT WITH' named entity 'PERFORMED' named entity 'VIRAL' named entity 'IN VITRO MODEL' named entity 'ANTIBODY TITRES' named entity 'PRIMED' named entity 'LYMPHOID TISSUES' named entity 'NUMBERS' named entity 'TESTS' named entity 'IN VITRO' named entity 'ELISA' named entity 'STIMULATION' named entity 'EARLIER' named entity 'VIRAL ANTIGEN' named entity 'DIFFERENT' named entity 'PROFILE' named entity 'CELLS' named entity 'MESENTERIC LYMPH NODE' named entity 'PIG' named entity 'GASTROENTERITIS' named entity 'ANTIBODY RESPONSE' named entity 'KINETICS' named entity 'RESPONSE TO' named entity 'VIRUS' named entity 'RESPONSES' named entity 'ELISA' named entity 'IN VITRO STUDIES' named entity 'ASC' named entity 'DEFINED' named entity 'DAYS' named entity 'REVEALED' named entity 'ANTIBODY' named entity 'REGULATION' named entity 'ORGANS' named entity 'CLOSE RELATIONSHIP' named entity 'ENUMERATION' named entity 'PERMIT' named entity 'PEROXIDASE' named entity 'OBSERVED' named entity 'PEAK' named entity 'CHARACTERIZED' named entity 'MECHANISMS' named entity 'RESULTS' named entity 'PIG' named entity 'USING' named entity 'BOOST' named entity 'IGA' named entity 'GASTROENTERITIS' named entity 'STUDIES' named entity 'ETHYL' named entity 'CARBAZOLE' named entity 'CULTURE' named entity 'MAINTAINED' named entity 'MESENTERIC LYMPH NODE' named entity 'LEVEL' named entity 'ANTIBODIES' named entity 'AMINO'

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