About: Identification of a Conserved B-cell Epitope on Reticuloendotheliosis Virus Envelope Protein by Screening a Phage-displayed Random Peptide Library

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About: Identification of a Conserved B-cell Epitope on Reticuloendotheliosis Virus Envelope Protein by Screening a Phage-displayed Random Peptide Library Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of a Conserved B-cell Epitope on Reticuloendotheliosis Virus Envelope Protein by Screening a Phage-displayed Random Peptide Library
Creator	Zhao, Yan Zhang, Jing Cui, Hongyu Hu, Shunlei Shi, Xingming Xue, Mei Cui, Xianlan Gao, Hongbo Wang, Yun-Feng
Source	PMC
abstract	BACKGROUND: The gp90 protein of avian reticuloendotheliosis-associated virus (REV-A) is an important envelope glycoprotein, which is responsible for inducing protective antibody immune responses in animals. B-cell epitopes on the gp90 protein of REV have not been well studied and reported. METHODS AND RESULTS: This study describes the identification of a linear B-cell epitope on the gp90 protein by screening a phage-displayed 12-mer random peptide library with the neutralizing monoclonal antibody (mAb) A9E8 directed against the gp90. The mAb A9E8 recognized phages displaying peptides with the consensus motif SVQYHPL. Amino acid sequence of the motif exactly matched (213)SVQYHPL(219) of the gp90. Further identification of the displayed B cell epitope was conducted using a set of truncated peptides expressed as GST fusion proteins and the Western blot results indicated that (213)SVQYHPL(219) was the minimal determinant of the linear B cell epitope recognized by the mAb A9E8. Moreover, an eight amino acid peptide SVQYHPLA was proven to be the minimal unit of the epitope with the maximal binding activity to mAb A9E8. The REV-A-positive chicken serum reacted with the minimal linear epitopes in Western blot, revealing the importance of the eight amino acids of the epitope in antibody-epitope binding activity. Furthermore, we found that the epitope is a common motif shared among REV-A and other members of REV group. CONCLUSIONS AND SIGNIFICANCE: We identified (213)SVQYHPL(219) as a gp90-specific linear B-cell epitope recognized by the neutralizing mAb A9E8. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against REV-A and other viruses of the REV group.
has issue date	2012-11-21(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0049842
bibo:pmid	23185456
has license	cc-by
sha1sum (hex)	0cc12d028d0f63383a4243bb73d76f70ae229daa
schema:url	https://doi.org/10.1371/journal.pone.0049842
resource representing a document's title	Identification of a Conserved B-cell Epitope on Reticuloendotheliosis Virus Envelope Protein by Screening a Phage-displayed Random Peptide Library
has PubMed Central identifier	PMC3504085
has PubMed identifier	23185456
schema:publication	PLoS One
resource representing a document's body	covid:0cc12d028d0f63383a4243bb73d76f70ae229daa#body_text
is schema:about of	named entity 'protein' named entity 'Protein' named entity 'Identification' named entity 'PHAGE' named entity 'HAVE' named entity 'B-CELL' named entity 'INDUCING' named entity 'GP90' named entity 'ASSOCIATED VIRUS' named entity 'BACKGROUND' named entity 'PROTEIN ' named entity 'REPORTED' named entity 'AVIAN RETICULOENDOTHELIOSIS' named entity 'ENVELOPE GLYCOPROTEIN' named entity 'ANTIBODY' named entity 'B-CELL EPITOPE' named entity 'IDENTIFICATION' named entity 'PEPTIDE LIBRARY' named entity 'CONSERVED' named entity 'VIRUS ENVELOPE' named entity 'DISPLAYED' named entity 'PROTEIN ' named entity 'ENVELOPE PROTEIN' named entity 'SCREENING' named entity 'RANDOM' named entity 'ANIMALS' named entity 'IMPORTANT' named entity 'EPITOPES' named entity 'RETICULOENDOTHELIOSIS VIRUS' named entity 'RESPONSIBLE' named entity 'REV' named entity 'IMMUNE RESPONSES' covid:arg/0cc12d028d0f63383a4243bb73d76f70ae229daa named entity 'studied' named entity 'B-cell' named entity 'Library' named entity 'Reticuloendotheliosis' named entity 'glycoprotein' named entity 'protein' named entity 'reticuloendotheliosis-associated virus' named entity 'epitopes' named entity 'mAb' named entity 'NaN3' named entity 'recombinant' named entity 'mAb' named entity 'phage' named entity 'protein' named entity 'sucrose' named entity 'fusion protein' named entity 'mice' named entity 'Japanese encephalitis' named entity 'A-positive' named entity 'viruses' named entity 'phages' named entity 'Enterovirus 71' named entity 'cross-reactivity' named entity 'protein' named entity 'heavy chain' named entity 'immunofluorescence assay' named entity 'Shanghai' named entity 'peptide' named entity 'Birmingham, AL' named entity 'antiserum' named entity 'monoclonal antibodies' named entity 'PBS' named entity 'microplate reader' named entity 'deionized water' named entity 'highly conserved' named entity 'absorbance' named entity 'linear epitope' named entity 'primer' named entity 'diagnostic tools' named entity 'Briey'

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