About: Porcine Reproductive and Respiratory Syndrome Virus Infection Induces Stress Granule Formation Depending on Protein Kinase R-like Endoplasmic Reticulum Kinase (PERK) in MARC-145 Cells

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About: Porcine Reproductive and Respiratory Syndrome Virus Infection Induces Stress Granule Formation Depending on Protein Kinase R-like Endoplasmic Reticulum Kinase (PERK) in MARC-145 Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Porcine Reproductive and Respiratory Syndrome Virus Infection Induces Stress Granule Formation Depending on Protein Kinase R-like Endoplasmic Reticulum Kinase (PERK) in MARC-145 Cells
Creator	Chen, Huanchun Fang, Liurong Wang, Dang Xiao, Shaobo Frossard, Jean-Pierre Cai, Kaimei Zhou, Yanrong Goodman, Alan Varga, Steven
source	Medline; PMC
abstract	Stress granules (SGs) are sites of mRNA storage that are formed in response to various conditions of stress, including viral infections. Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus that has been devastating the swine industry worldwide since the late 1980s. In this study, we found that infection of PRRSV strain WUH3 (genotype 2 PRRSV) induced stable formation of robust SGs in MARC-145 cells, as demonstrated by the recruitment of marker proteins of SGs, including TIA1, G3BP1, and eIF3η. Treatment with specific inhibitors or siRNAs against the stress kinases that are involved in SG formation revealed that PRRSV induced SG formation through a PERK (protein kinase R–like endoplasmic reticulum kinase)-dependent mechanism. Impairment of SG assembly by concomitant knockdown of the SG marker proteins (TIA1, G3BP1, and TIAR) did not affect PRRSV growth, while significantly enhanced PRRSV-induced NF-κB subunit p65 phosphorylation and inflammatory cytokine production. Taken together, our results demonstrate that PRRSV induces SG formation via a PERK-dependent pathway and that SGs are involved in the signaling pathway of the PRRSV-induced inflammatory response in MARC-145 cells.
has issue date	2017-04-04(xsd:dateTime)
bibo:doi	10.3389/fcimb.2017.00111
bibo:pmid	28421170
has license	cc-by
sha1sum (hex)	0c589675855cd6a533025c50fb319ecd269c2d0d
schema:url	https://doi.org/10.3389/fcimb.2017.00111
resource representing a document's title	Porcine Reproductive and Respiratory Syndrome Virus Infection Induces Stress Granule Formation Depending on Protein Kinase R-like Endoplasmic Reticulum Kinase (PERK) in MARC-145 Cells
has PubMed Central identifier	PMC5378712
has PubMed identifier	28421170
schema:publication	Front Cell Infect Microbiol
resource representing a document's body	covid:0c589675855cd6a533025c50fb319ecd269c2d0d#body_text
is schema:about of	named entity 'stress' named entity 'respiratory' named entity 'TIA1' named entity 'late' named entity 'inflammatory response' named entity 'Infection' named entity 'Endoplasmic Reticulum' named entity 'INHIBITORS' named entity 'STRESS' named entity 'SG ASSEMBLY' named entity 'MARKER' named entity 'INFLAMMATORY RESPONSE' named entity 'INFLAMMATORY CYTOKINE' named entity 'CONCOMITANT' named entity 'RESULTS' named entity 'TIA1' named entity 'FORMED' named entity 'G3BP1' named entity 'BAB' named entity 'STABLE' named entity 'FORMATION' named entity 'VIRAL INFECTIONS' named entity 'ENHANCED' named entity 'PROTEINS' named entity 'INDUCED' named entity 'PATHWAY' named entity 'KINASES' named entity 'TAKEN' named entity 'GRANULES' named entity 'INFECTION' named entity 'SITES' named entity 'STRAIN' named entity 'STORAGE' named entity 'SPECIFIC' named entity 'DEPENDENT' named entity 'DEMONSTRATED' named entity 'LIKE' named entity 'REVEALED' named entity 'OUR' named entity 'TIAR' named entity 'KINASE' named entity 'ARTERIVIRUS' named entity 'FORMATION' named entity 'STRESS GRANULE' named entity 'EIF3' named entity 'FOUND' named entity 'RESPONSE TO' named entity 'WORLDWIDE' named entity 'MARC' named entity 'SIGNALING PATHWAY' named entity 'ENDOPLASMIC RETICULUM' named entity 'KINASE' named entity 'LIKE' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'CITATION' named entity '145' named entity 'ENDOPLASMIC RETICULUM' named entity 'VIRUS INFECTION' named entity 'INCLUDING' named entity 'SUBUNIT' named entity 'INDUSTRY' named entity 'PHOSPHORYLATION' named entity 'TREATMENT' named entity 'LATE' named entity 'PERK' named entity 'INVOLVED' named entity 'IMPAIRMENT' named entity 'AFFECT' named entity 'SIRNAS' named entity 'CELLS' named entity 'VARIOUS' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'CONDITIONS'

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