About: Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases
Creator	Bossers, Alex Smid, Bregtje Antonis, Adriaan Cornelissen, Jan De Bree, Freddy Koene, Miriam Kooi, Engbert Van Der Wal, Fimme Wisselink, Henk
Source	Medline; PMC
abstract	BACKGROUND: In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. bovis and M. bovirhinis, all three associated with bovine respiratory disease (BRD). Primers and probes of the RespoCheck Mycoplasma triplex real-time PCR are based on the V3/V4 region of the 16S rRNA gene of the three Mycoplasma species. RESULTS: The analytical sensitivity of the RespoCheck triplex real-time PCR was, as determined by spiking experiments of the Mycoplasma strains in Phosphate Buffered Saline, 300 colony forming units (cfu)/mL for M. dispar, and 30 cfu/mL for M. bovis or M. bovirhinis. The analytical sensitivity of the RespoCheck Mycoplasma triplex real-time PCRwas, as determined on purified DNA, 10 fg DNA per assay for M. dispar and 100 fg fo rM. bovis and M. bovirhinis. The analytical specificity of the RespoCheck Mycoplasma triplex real-time PCR was, as determined by testing Mycoplasmas strains (n = 17) and other bacterial strains (n = 107), 100, 98.2 and 99.1% for M. bovis, M. dispar and M. bovirhinis respectively. The RespoCheck Mycoplasma triplex real-time PCR was compared with the PCR/DGGE analysis for M. bovis, M. dispar and M. bovirhinis respectively by testing 44 BALF samples from calves. CONCLUSION: In conclusion, the RespoCheck PCR assay can be a valuable tool for timely and accurate detection of three Mycoplasma species associated with in bovine respiratory disease.
has issue date	2017-04-08(xsd:dateTime)
bibo:doi	10.1186/s12917-017-1023-6
bibo:pmid	28390431
has license	cc-by
sha1sum (hex)	0b7581ab8ae5da78a11692b4c7d9399ec6dea283
schema:url	https://doi.org/10.1186/s12917-017-1023-6
resource representing a document's title	Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases
has PubMed Central identifier	PMC5385097
has PubMed identifier	28390431
schema:publication	BMC Vet Res
resource representing a document's body	covid:0b7581ab8ae5da78a11692b4c7d9399ec6dea283#body_text
is schema:about of	named entity 'ACCURATE' named entity 'PROBES' named entity 'DETERMINED BY' named entity 'PURIFIED' named entity 'PRIMERS' named entity 'SAMPLES' named entity 'EXPERIMENTS' named entity 'CONCLUSION' named entity 'TIMELY' named entity 'MYCOPLASMA' named entity '100%' named entity 'REGION' named entity 'SPECIES' named entity 'PCR ASSAY' named entity 'ANALYTICAL' named entity 'specificity' named entity 'M. bovis' named entity 'real-time PCR' named entity 'real-time PCR' named entity 'Mycoplasma' named entity 'CASES' named entity 'PHOSPHATE BUFFERED SALINE' named entity 'MYCOPLASMAS' named entity 'SPIKING' named entity 'BACTERIAL' named entity 'BASED' named entity 'COLONY FORMING UNITS' named entity 'EVALUATED' named entity 'BOVIS' named entity '100' named entity 'CALVES' named entity 'SPECIFICITY' named entity 'STRAINS' named entity 'RESULTS' named entity 'BRONCHOALVEOLAR LAVAGE FLUID' named entity 'BACKGROUND' named entity '300' named entity '107' named entity 'COMPARED' named entity 'DETECTION' named entity 'MYCOPLASMA' named entity 'REAL-TIME PCR ' named entity 'BOVINE RESPIRATORY DISEASE COMPLEX' named entity 'ANALYSIS' named entity 'CFU' named entity 'PCR' named entity '16S RRNA GENE' named entity 'BRONCHOALVEOLAR LAVAGE FLUID' named entity 'DISPAR' named entity 'REAL-TIME PCR ' named entity 'TESTING' named entity 'DETECTION' named entity 'REAL-TIME' named entity 'BOVINE RESPIRATORY DISEASE' named entity 'TOOL' named entity 'ASSOCIATED WITH' named entity 'DETERMINED' named entity 'DNA' named entity 'STUDY' named entity 'SENSITIVITY' named entity 'ASSAY' named entity 'strains' named entity 'compared' named entity 'Primers' named entity 'study' named entity 'real-time PCR' named entity 'real-time PCR' named entity 'testing' named entity 'gene' named entity 'accurate' named entity 'BALF' named entity 'BRD' named entity 'region'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software