About: Membrane protein assembly: two cytoplasmic phosphorylated serine sites of Vpu from HIV-1 affect oligomerization

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About: Membrane protein assembly: two cytoplasmic phosphorylated serine sites of Vpu from HIV-1 affect oligomerization Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Membrane protein assembly: two cytoplasmic phosphorylated serine sites of Vpu from HIV-1 affect oligomerization
Creator	Chan, Ya-Ting Chen, Chin-Pei Chen, Li-Chyong Lin, Meng-Han Ma, Che Fischer, Wolfgang
Source	PMC
abstract	Viral protein U (Vpu) encoded by human immunodeficiency virus type 1 (HIV-1) is a short integral membrane protein which is known to self-assemble within the lipid membrane and associate with host factors during the HIV-1 infectivity cycle. In this study, full-length Vpu (M group) from clone NL4-3 was over-expressed in human cells and purified in an oligomeric state. Various single and double mutations were constructed on its phosphorylation sites to mimic different degrees of phosphorylation. Size exclusion chromatography of wild-type Vpu and mutants indicated that the smallest assembly unit of Vpu was a dimer and over time Vpu formed higher oligomers. The rate of oligomerization increased when (i) the degree of phosphorylation at serines 52 and 56 was decreased and (ii) when the ionic strength was increased indicating that the cytoplasmic domain of Vpu affects oligomerization. Coarse-grained molecular dynamic simulations with models of wild-type and mutant Vpu in a hydrated lipid bilayer supported the experimental data in demonstrating that, in addition to a previously known role in downregulation of host factors, the phosphorylation sites of Vpu also modulate oligomerization.
has issue date	2016-06-29(xsd:dateTime)
bibo:doi	10.1038/srep28866
bibo:pmid	27353136
has license	cc-by
sha1sum (hex)	09242b23825a9c7e09660df99494b673e5abea3e
schema:url	https://doi.org/10.1038/srep28866
resource representing a document's title	Membrane protein assembly: two cytoplasmic phosphorylated serine sites of Vpu from HIV-1 affect oligomerization
has PubMed Central identifier	PMC4926278
has PubMed identifier	27353136
schema:publication	Sci Rep
resource representing a document's body	covid:09242b23825a9c7e09660df99494b673e5abea3e#body_text
is schema:about of	named entity 'PHOSPHORYLATION SITES' named entity 'DOWNREGULATION' named entity 'MODULATE' named entity 'OLIGOMERIZATION' named entity 'downregulation' named entity 'modulate' named entity 'serine' named entity 'OPEN' named entity 'BILAYER' named entity 'KNOWN' named entity 'ADDITION' named entity 'VPU' named entity 'SITES' named entity 'SERINE' named entity 'MEMBRANE PROTEIN' named entity 'OLIGOMERIZATION' named entity 'AFFECT' named entity 'EXPERIMENTAL DATA' named entity 'SUPPORTED' named entity 'PHOSPHORYLATED' named entity 'HIV-1' named entity 'CYTOPLASMIC' named entity 'PROTEIN ASSEMBLY' named entity 'PREVIOUSLY' named entity 'VPU' named entity 'HOST' named entity 'ROLE' named entity 'downregulation' named entity 'phosphorylation sites' named entity 'Membrane protein' named entity 'HIV-1' named entity 'phosphorylated' named entity 'cytoplasmic' named entity 'oligomerization' named entity 'lipid' named entity 'lipid' named entity 'Oligomerization' named entity 'Vpu' named entity 'Viral' named entity 'Vpu' named entity 'GLIC' named entity 'Vpu' named entity 'cytoplasmic' named entity 'Glu' named entity 'Vpu' named entity 'oligomer' named entity 'helical' named entity 'protein' named entity 'gel permeation chromatography' named entity 'cytoplasmic domain' named entity 'protein' named entity 'Vpu' named entity 'lipids' named entity 'oligomers' named entity 'planar lipid bilayer' named entity 'protein' named entity 'lipid' named entity 'detergent' named entity 'logistic growth' named entity 'helix' named entity 'dimer' named entity 'Vpu' named entity '1.2' named entity 'oligomer' named entity 'Vpu' named entity 'dimers' named entity 'Vpu' named entity 'Vpu' named entity 'planar lipid bilayer' named entity 'helical' named entity 'serine' named entity 'Vpu' named entity 'oligomerization' named entity 'oligomer' named entity 'Vpu' named entity 'Vpu'

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