About: Serodiagnosis of Echinococcus spp. Infection: Explorative Selection of Diagnostic Antigens by Peptide Microarray

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About: Serodiagnosis of Echinococcus spp. Infection: Explorative Selection of Diagnostic Antigens by Peptide Microarray Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Serodiagnosis of Echinococcus spp. Infection: Explorative Selection of Diagnostic Antigens by Peptide Microarray
Creator	Felger, Ingrid Gottstein, Bruno List, Claudia Maag, Eva Qi, Weihong Mü Ller, Norbert
topic	covid:08174b668145c88e0e719dac2a03b30fb68f5de5#this
Source	PMC
abstract	BACKGROUND: Production of native antigens for serodiagnosis of helminthic infections is laborious and hampered by batch-to-batch variation. For serodiagnosis of echinococcosis, especially cystic disease, most screening tests rely on crude or purified Echinococcus granulosus hydatid cyst fluid. To resolve limitations associated with native antigens in serological tests, the use of standardized and highly pure antigens produced by chemical synthesis offers considerable advantages, provided appropriate diagnostic sensitivity and specificity is achieved. METHODOLOGY/PRINCIPAL FINDINGS: Making use of the growing collection of genomic and proteomic data, we applied a set of bioinformatic selection criteria to a collection of protein sequences including conceptually translated nucleotide sequence data of two related tapeworms, Echinococcus multilocularis and Echinococcus granulosus. Our approach targeted alpha-helical coiled-coils and intrinsically unstructured regions of parasite proteins potentially exposed to the host immune system. From 6 proteins of E. multilocularis and 5 proteins of E. granulosus, 45 peptides between 24 and 30 amino acids in length were designed. These peptides were chemically synthesized, spotted on microarrays and screened for reactivity with sera from infected humans. Peptides reacting above the cut-off were validated in enzyme-linked immunosorbent assays (ELISA). Peptides identified failed to differentiate between E. multilocularis and E. granulosus infection. The peptide performing best reached 57% sensitivity and 94% specificity. This candidate derived from Echinococcus multilocularis antigen B8/1 and showed strong reactivity to sera from patients infected either with E. multilocularis or E. granulosus. CONCLUSIONS/SIGNIFICANCE: This study provides proof of principle for the discovery of diagnostically relevant peptides by bioinformatic selection complemented with screening on a high-throughput microarray platform. Our data showed that a single peptide cannot provide sufficient diagnostic sensitivity whereas pooling several peptide antigens improved sensitivity; thus combinations of several peptides may lead the way to new diagnostic tests that replace, or at least complement conventional immunodiagnosis of echinococcosis. Our strategy could prove useful for diagnostic developments in other pathogens.
has issue date	2010-08-03(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0000771
bibo:pmid	20689813
has license	cc-by
sha1sum (hex)	08174b668145c88e0e719dac2a03b30fb68f5de5
schema:url	https://doi.org/10.1371/journal.pntd.0000771
resource representing a document's title	Serodiagnosis of Echinococcus spp. Infection: Explorative Selection of Diagnostic Antigens by Peptide Microarray
has PubMed Central identifier	PMC2914747
has PubMed identifier	20689813
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:08174b668145c88e0e719dac2a03b30fb68f5de5#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/08174b668145c88e0e719dac2a03b30fb68f5de5
is schema:about of	named entity 'INFECTION' named entity 'serological tests' named entity 'fluid' named entity 'helminthic' named entity 'For' named entity 'CONSIDERABLE' named entity 'HIGHLY' named entity 'PRODUCTION' named entity 'PURIFIED' named entity 'USE OF' named entity 'ASSOCIATED WITH' named entity 'CYSTIC DISEASE' named entity 'SCREENING TESTS' named entity 'CHEMICAL SYNTHESIS' named entity 'ECHINOCOCCUS GRANULOSUS' named entity 'PURE' named entity 'DIAGNOSTIC SENSITIVITY' named entity 'VARIATION' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'CYST FLUID' named entity 'ANTIGENS' named entity 'BACKGROUND' named entity 'SEROLOGICAL TESTS' named entity 'BATCH' named entity 'HELMINTHIC INFECTIONS' named entity 'SERODIAGNOSIS' named entity 'LIMITATIONS' named entity 'SPP' named entity 'MICROARRAY' named entity 'SELECTION' named entity 'APPROPRIATE' named entity 'ECHINOCOCCUS' named entity 'DIAGNOSTIC ANTIGENS' named entity 'PROVIDED' named entity 'PEPTIDE' named entity 'NATIVE' named entity 'RESOLVE' named entity 'HYDATID CYST' named entity 'SERODIAGNOSIS' named entity 'ECHINOCOCCOSIS' covid:arg/08174b668145c88e0e719dac2a03b30fb68f5de5 named entity 'rely' named entity 'disease' named entity 'echinococcosis' named entity 'produced' named entity 'variation' named entity 'antigens' named entity 'serological tests' named entity 'serodiagnosis' named entity 'sensitivity and specificity' named entity 'helminthic' named entity 'serodiagnosis' named entity 'native' named entity 'Switzerland' named entity 'microarrays' named entity 'peptide' named entity 'antigens' named entity 'IgG' named entity 'immunoblots' named entity 'genomes' named entity 'IgG antibodies' named entity 'cut-off' named entity 'diagnostic assays' named entity 'EST libraries' named entity 'peptide' named entity 'recombinant' named entity 'peptides' named entity 'structural motifs' named entity 'standard deviations' named entity 'cut-off' named entity 'Schistosoma mansoni' named entity 'chemically synthesized' named entity 'peptides' named entity 'coiled-coil'

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