About: Simply saliva: stability of SARS-CoV-2 detection negates the need for expensive collection devices

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About: Simply saliva: stability of SARS-CoV-2 detection negates the need for expensive collection devices Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simply saliva: stability of SARS-CoV-2 detection negates the need for expensive collection devices
Creator	Iwasaki, Akiko Grubaugh, Nathan Kalinich, Chaney Ko, Albert Ott, Isabel Vogels, Chantal Wilen, Craig Wyllie, Anne Cruz, Dela Bermejo, Santos Casanovas-Massana, Arnau Farhadian, Shelli Fournier, John Moore, Adam Nakahata, Maura Tokuyama, Maria Watkins, Anne Boot, Maikel Campbell, Melissa Datta, Rupak Harden, Christina Muenker, M Nelson, Allison Strine, Madison
Source	MedRxiv; Medline; PMC
abstract	Most currently approved strategies for the collection of saliva for COVID-19 diagnostics require specialized tubes containing buffers promoted for the stabilization of SARS-CoV-2 RNA and virus inactivation. Yet many of these are expensive, in limited supply, and not necessarily validated specifically for viral RNA. While saliva is a promising sample type as it can be reliably self-collected for the sensitive detection of SARS-CoV-2, the expense and availability of these collection tubes are prohibitive to mass testing efforts. Therefore, we investigated the stability of SARS-CoV-2 RNA and infectious virus detection from saliva without supplementation. We tested RNA stability over extended periods of time (2-25 days) and at temperatures representing at-home storage and elevated temperatures which might be experienced when cold chain transport may be unavailable. We found SARS-CoV-2 RNA in saliva from infected individuals is stable at 4°C, room temperature (~19°C), and 30°C for prolonged periods and found limited evidence for viral replication in stored saliva samples. This work demonstrates that expensive saliva collection options involving RNA stabilization and virus inactivation buffers are not always needed, permitting the use of cheaper collection options. Affordable testing methods are urgently needed to meet current testing demands and for continued surveillance in reopening strategies.
has issue date	2020-08-04(xsd:dateTime)
bibo:doi	10.1101/2020.08.03.20165233
bibo:pmid	32793924
has license	cc-by
sha1sum (hex)	063e38e3e1780caf0fd1559427f334c58a9f3e15
schema:url	https://doi.org/10.1101/2020.08.03.20165233
resource representing a document's title	Simply saliva: stability of SARS-CoV-2 detection negates the need for expensive collection devices
has PubMed Central identifier	PMC7418742
has PubMed identifier	32793924
schema:publication	medRxiv
resource representing a document's body	covid:063e38e3e1780caf0fd1559427f334c58a9f3e15#body_text
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