About: Spacer-length dependence of programmed −1 or −2 ribosomal frameshifting on a U(6)A heptamer supports a role for messenger RNA (mRNA) tension in frameshifting

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About: Spacer-length dependence of programmed −1 or −2 ribosomal frameshifting on a U(6)A heptamer supports a role for messenger RNA (mRNA) tension in frameshifting Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Spacer-length dependence of programmed −1 or −2 ribosomal frameshifting on a U(6)A heptamer supports a role for messenger RNA (mRNA) tension in frameshifting
Creator	Brierley, Ian Gilbert, Robert Lin, Zhaoru
Source	Medline; PMC
abstract	Programmed −1 ribosomal frameshifting is employed in the expression of a number of viral and cellular genes. In this process, the ribosome slips backwards by a single nucleotide and continues translation of an overlapping reading frame, generating a fusion protein. Frameshifting signals comprise a heptanucleotide slippery sequence, where the ribosome changes frame, and a stimulatory RNA structure, a stem–loop or RNA pseudoknot. Antisense oligonucleotides annealed appropriately 3′ of a slippery sequence have also shown activity in frameshifting, at least in vitro. Here we examined frameshifting at the U(6)A slippery sequence of the HIV gag/pol signal and found high levels of both −1 and −2 frameshifting with stem–loop, pseudoknot or antisense oligonucleotide stimulators. By examining −1 and −2 frameshifting outcomes on mRNAs with varying slippery sequence-stimulatory RNA spacing distances, we found that −2 frameshifting was optimal at a spacer length 1–2 nucleotides shorter than that optimal for −1 frameshifting with all stimulatory RNAs tested. We propose that the shorter spacer increases the tension on the mRNA such that when the tRNA detaches, it more readily enters the −2 frame on the U(6)A heptamer. We propose that mRNA tension is central to frameshifting, whether promoted by stem–loop, pseudoknot or antisense oligonucleotide stimulator.
has issue date	2012-06-28(xsd:dateTime)
bibo:doi	10.1093/nar/gks629
bibo:pmid	22743270
has license	cc-by-nc
sha1sum (hex)	06137bb1e53080493b33c1b749b59848cddc05fc
schema:url	https://doi.org/10.1093/nar/gks629
resource representing a document's title	Spacer-length dependence of programmed −1 or −2 ribosomal frameshifting on a U(6)A heptamer supports a role for messenger RNA (mRNA) tension in frameshifting
has PubMed Central identifier	PMC3458567
has PubMed identifier	22743270
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:06137bb1e53080493b33c1b749b59848cddc05fc#body_text
is schema:about of	named entity 'EMPLOYED' named entity 'SLIPS' named entity 'RIBOSOMAL' named entity 'READING FRAME' named entity 'EXPRESSION' named entity 'number' named entity 'TENSION' covid:arg/06137bb1e53080493b33c1b749b59848cddc05fc named entity 'MESSENGER RNA' named entity 'CELLULAR' named entity 'ROLE' named entity 'NUCLEOTIDE' named entity 'GENERATING' named entity 'TRANSLATION' named entity 'HEPTAMER' named entity 'SPACER' named entity 'DEPENDENCE' named entity 'PROCESS' named entity 'OVERLAPPING' named entity 'SINGLE' named entity 'RIBOSOMAL' named entity 'LENGTH' named entity 'NUMBER OF' named entity 'VIRAL AND' named entity 'GENES' named entity 'BACKWARDS' named entity 'FUSION PROTEIN' named entity 'RIBOSOME' named entity 'nucleotide' named entity 'genes' named entity 'fusion protein' named entity 'continues' named entity 'reading frame' named entity 'messenger RNA' named entity 'frameshifting' named entity 'RNA' named entity 'pseudoknot' named entity 'U 6' named entity 'ribosome' named entity '3, 4' named entity 'mRNA' named entity 'MG132' named entity 'slippery sequence' named entity 'eGFP' named entity 'frameshift' named entity 'transfected' named entity 'slippery sequence' named entity 'Colorado' named entity 'mRNA' named entity 'plasmid' named entity 'annealed' named entity 'RNA' named entity 'polymerase' named entity 'stem-loop' named entity 'mammals' named entity 'AON' named entity 'Escherichia coli' named entity 'tRNAs' named entity 'base-pairs' named entity 'pH 8' named entity 'virus' named entity 'T-cell' named entity 'heptameric' named entity 'P-site' named entity 'gene' named entity 'mRNA' named entity 'incubation' named entity 'luciferases' named entity 'thermodynamic stability' named entity 'heptamer' named entity 'mRNAs' named entity 'slippery sequence' named entity 'ribosomes' named entity 'HIV-1' named entity 'plasmid' named entity 'virus' named entity 'plasmids' named entity 'frameshift' named entity 'frameshifting'

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