About: Novel rapid identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by real-time RT-PCR using BD Max Open System in Taiwan

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About: Novel rapid identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by real-time RT-PCR using BD Max Open System in Taiwan Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Novel rapid identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by real-time RT-PCR using BD Max Open System in Taiwan
Creator	Chang, Feng-Yee Chang, Chih-Kai Lin, Jung-Chung Perng, Cherng-Lih Yeh, Kuo-Ming Chen, Chien-Wen Chiu, Sheng-Kang Chung, Hsing-Yi Hsieh, Shan-Shan Li, Shih-Yi Liao, Shu-Jung Shang, Hung-Sheng Tsai, Shih-Hung Wang, Yi-Hui Jian, Ming-
Source	Medline; PMC; WHO
abstract	Coronavirus disease 2019 has become a worldwide pandemic. By April 7, 2020, approximately 1,279,722 confirmed cases were reported worldwide including those in Asia, European Region, African Region and Region of the Americas. Rapid and accurate detection of Severe Acute Respiratory Syndrome Virus 2 (SARS-CoV-2) is critical for patient care and implementing public health measures to control the spread of infection. In this study, we developed and validated a rapid total nucleic acid extraction method based on real‐time RT-PCR for reliable, high‐throughput identification of SARS-CoV-2 using the BD MAX platform. For clinical validation, 300 throat swab and 100 sputum clinical samples were examined by both the BD MAX platform and in-house real-time RT-PCR methods, which showed 100% concordant results. This BD MAX protocol is fully automated and the turnaround time from sample to results is approximately 2.5 h for 24 samples compared to 4.8 h by in-house real-time RT-PCR. Our developed BD MAX RT-PCR assay can accurately identify SARS-CoV-2 infection and shorten the turnaround time to increase the effectiveness of control and prevention measures for this emerging infectious disease.
has issue date	2020-06-17(xsd:dateTime)
bibo:doi	10.7717/peerj.9318
bibo:pmid	32596046
has license	cc-by
sha1sum (hex)	05197f2d904fba4b6f267677edda9b1d43d95ef5
schema:url	https://doi.org/10.7717/peerj.9318
resource representing a document's title	Novel rapid identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by real-time RT-PCR using BD Max Open System in Taiwan
has PubMed Central identifier	PMC7305773
has PubMed identifier	32596046
schema:publication	PeerJ
resource representing a document's body	covid:05197f2d904fba4b6f267677edda9b1d43d95ef5#body_text
is schema:about of	named entity 'platform' named entity 'SARS-CoV-2' named entity 'Severe Acute Respiratory Syndrome' named entity 'RT-PCR ASSAY' named entity 'EXTRACTION METHOD' named entity 'FOR PATIENT' named entity 'REPORTED' named entity 'RAPID' named entity '4.8' named entity 'FULLY' named entity 'AMERICAS' named entity 'TOTAL' named entity 'COMPARED' named entity 'SHORTEN' named entity 'RELIABLE' named entity 'VIRUS' named entity 'OUR' named entity 'APRIL' named entity 'TO CONTROL' named entity 'compared' named entity 'validation' named entity 'acid' named entity 'pandemic' named entity 'infection' named entity 'care' named entity 'cases' named entity 'turnaround time' named entity 'rapid' named entity 'measures' named entity 'Taiwan' named entity 'SARS-CoV-2' named entity 'worldwide pandemic' named entity 'Severe Acute Respiratory Syndrome Virus' named entity 'SARS-CoV-2' named entity 'assay' named entity 'turnaround time' named entity 'nucleic acid' named entity 'reverse transcription' named entity 'Taipei' named entity 'sputum' named entity 'Hilden' named entity 'CDC' named entity 'RT-PCR' named entity 'World Health Organization' named entity '1.2' named entity 'pathogens' named entity 'RNA' named entity '5′→3′' named entity 'infection' named entity 'London' named entity 'RdRp' named entity 'SARS-CoV-2 pandemic' named entity 'SARS-CoV-2' named entity 'turnaround time' named entity 'viral RNA' named entity 'Institutional Review Board' named entity 'real-time RT-PCR' named entity 'RT-PCR' named entity 'primer' named entity 'SARS-CoV-2' named entity 'PCR' named entity 'primers' named entity 'SARS-CoV-2' named entity 'primers' named entity 'RdRp' named entity 'SARS-CoV-2' named entity 'RT-PCR'

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