About: Crystal structure of the SARS-CoV-2 non-structural protein 9, Nsp9

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About: Crystal structure of the SARS-CoV-2 non-structural protein 9, Nsp9 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Crystal structure of the SARS-CoV-2 non-structural protein 9, Nsp9
Creator	Rossjohn, &
Source	BioRxiv
abstract	Many of the proteins produced by SARS-CoV-2 have related counterparts across the Severe Acute Respiratory Syndrome (SARS-CoV) family. One such protein is non-structural protein 9 (Nsp9), which is thought to mediate both viral replication and virulence. Current understanding suggests that Nsp9 is involved in viral genomic RNA reproduction. Nsp9 is thought to bind RNA via a fold that is unique to this class of betacoronoaviruses although the molecular basis for this remains ill-defined. We sought to better characterise the SARS-CoV-2 Nsp9 protein and subsequently solved its X-ray crystal structure, in an apo-form and, unexpectedly, in a peptide-bound form with a sequence originating from a rhinoviral 3C protease sequence (LEVL). The structure of the SARS-CoV-2 Nsp9 revealed the high level of structural conservation within the Nsp9 family. The exogenous peptide binding site is close to the dimer interface and impacted on the relative juxtaposition of the monomers within the homodimer. Together we have established a protocol for the production of SARS-CoV-2 Nsp9, determined its structure and identified a peptide-binding site that may warrant further study from the perspective of understanding Nsp9 function.
has issue date	2020-03-30(xsd:dateTime)
bibo:doi	10.1101/2020.03.28.013920
has license	biorxiv
sha1sum (hex)	034002bf1e872fdc2e4c0c2bfaac8349eef7ea72
schema:url	https://doi.org/10.1101/2020.03.28.013920
resource representing a document's title	Crystal structure of the SARS-CoV-2 non-structural protein 9, Nsp9
schema:publication	bioRxiv
resource representing a document's body	covid:034002bf1e872fdc2e4c0c2bfaac8349eef7ea72#body_text
is schema:about of	named entity 'produced' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'bind' named entity 'molecular' named entity 'protease' named entity 'reproduction' named entity 'dimer' named entity 'function' named entity 'Many' named entity 'structural' named entity 'protein' named entity 'RNA' named entity 'SARS-CoV' named entity 'homodimer' named entity 'binding site' named entity 'RNA' named entity 'non-structural protein' named entity 'Crystal structure' named entity 'oligonucleotide' named entity 'motif' named entity 'rhinoviral' named entity 'Rhinovirus' named entity 'His-tag' named entity 'RNA' named entity 'hydrophobic' named entity 'RNA binding' named entity 'binding affinities' named entity 'RNA binding' named entity 'SARS-CoV-2' named entity 'molecular basis' named entity 'dimer' named entity 'RNA-binding' named entity 'dimer' named entity 'cysteine' named entity 'size-exclusion chromatography' named entity 'a-helix' named entity 'affinity column' named entity 'C-terminal' named entity 'motif' named entity 'protomer' named entity 'oligonucleotides' named entity 'oligonucleotides' named entity 'porcine epidemic diarrhea virus' named entity 'motif' named entity 'consensus sequences' named entity 'cleavage site' named entity 'pH 4' named entity 'viral proteins' named entity 'Absorbance' named entity 'plasmid' named entity 'hydrophobic' named entity 'exogenous' named entity 'helix' named entity 'viruses' named entity 'binding affinity' named entity 'HEPES' named entity 'Fluorescein' named entity 'motif' named entity 'dimer' named entity 'coronaviruses' named entity 'hydrophobic' named entity 'Phosphate' named entity 'eluted' named entity 'space group' named entity 'β-sheet' named entity 'substrate' named entity 'motif' named entity 'single-stranded' named entity 'protein' named entity 'Imidazole' named entity 'dimerisation' named entity 'serine' named entity 'polyprotein' named entity 'crystallographic' named entity 'sequence identity' named entity 'open reading frames' named entity 'nucleotide' named entity 'N-1' named entity 'binding activity' named entity 'cloned' named entity 'fluorescently labelled' named entity 'helix' named entity 'protein'

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