About: Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses

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About: Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses
Creator	Kumar, Neeraj Malhotra, Bharti Reddy, P Swamy, M Tiwari, Jitendra
topic	covid:023858d3191988a37587ab951db561a3db3ae2cd#this
Source	Medline; PMC
abstract	BACKGROUND: Severe acute respiratory infections in children can be fatal, rapid identification of the causative agent and timely treatment can be life saving. Multiplex real time RT-PCR helps in simultaneous detection of multiple viruses saving cost, time and labour. Commercially available multiplex real time RT-PCR kits are very expensive. Therefore the aim of the present study was to develop a cost effective multiplex real time RT-PCR for the detection of 18 respiratory viruses and compare it with an in-vitro diagnostics approved Fast Track Diagnostic Respiratory Pathogens 21 Kit (FTD). METHODS: Nasopharyngeal aspirates and throat swabs were collected and processed for extraction of nucleic acid using an automated extraction system and multiplex real time RT-PCR was performed using the FTD kit and a custom assay on 356 samples. RESULTS: Custom and FTD assays detected one or more respiratory viruses in 268 (75.29 %) and 262 (73.60 %) samples respectively. The concordance between the custom assay and the FTD assay was 100 % for HCoV OC43, HCoV 229E, HPIV-1, HPIV-2, HBoV, HPeV, Flu A, and Influenza A(H1N1)pdm09 and 94.66 – 99.71 % for the remaining viruses; Flu B (99.71 %), HRV (99.71 %), HPIV-3 (98.87 %), HPIV-4 (99.43 %), HCoV NL63 (99.71 %), HMPV A/B (99.71 %), RSV A/B (94.66 %), EV (98.31 %), HCoV HKU1 (99.71 %), HAdV (99.71 %). Major discrepancy was observed for RSV A/B, which was over detected in 18 samples by the custom assay as compared to the FTD assay. The custom assay was much cheaper than the FTD assay and the time taken was only 29 min more. CONCLUSION: The custom primer and probe mix was found to be comparable to the FTD assay with good concordance but was much cheaper and the time taken for reporting was only 29 min more. The low cost custom multiplex RT-PCR can be a useful alternative to the costly FTD kit for rapid identification of viral aetiology in resource limited settings.
has issue date	2016-06-06(xsd:dateTime)
bibo:doi	10.1186/s12985-016-0549-8
bibo:pmid	27267595
has license	cc-by
sha1sum (hex)	023858d3191988a37587ab951db561a3db3ae2cd
schema:url	https://doi.org/10.1186/s12985-016-0549-8
resource representing a document's title	Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses
has PubMed Central identifier	PMC4896093
has PubMed identifier	27267595
schema:publication	Virol J
resource representing a document's body	covid:023858d3191988a37587ab951db561a3db3ae2cd#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/023858d3191988a37587ab951db561a3db3ae2cd
is schema:about of	named entity 'RT-PCR' named entity 'real time' named entity 'FTD' named entity 'helps' named entity 'detection' covid:arg/023858d3191988a37587ab951db561a3db3ae2cd named entity 'simultaneous' named entity 'real time' named entity 'Pathogens' named entity 'Methods' named entity 'causative' named entity 'identification' named entity 'develop' named entity 'infection' named entity 'RT-PCR' named entity 'Nasopharyngeal' named entity 'in-vitro' named entity 'Pakistan' named entity 'picomoles' named entity 'HRV' named entity 'throat swab' named entity 'HCoV NL63' named entity 'Bangladesh' named entity 'RSV' named entity 'viruses' named entity 'human Parainfluenza Virus' named entity 'death among children' named entity 'viruses' named entity 'HCoV HKU1' named entity 'nucleic acid' named entity 'human Rhinovirus' named entity 'viruses' named entity 'nucleic acids' named entity 'China' named entity 'HPIV-1' named entity 'RSV' named entity 'Respiratory Syncytial Virus' named entity 'primers' named entity 'RSV' named entity 'Influenza B Virus' named entity 'respiratory ailments' named entity 'viruses' named entity 'HCoV HKU1' named entity 'nucleic acid' named entity 'nucleic acid' named entity 'HPIV-1' named entity 'Influenza' named entity 'PCR' named entity 'Influenza' named entity 'acute respiratory infections' named entity 'viruses' named entity 'viruses' named entity 'multiplex PCR' named entity 'Influenza' named entity 'Jaipur' named entity 'positive control' named entity 'RSV' named entity 'RSV' named entity 'positive control' named entity 'Virology' named entity 'viruses' named entity 'India' named entity 'human Metapneumovirus' named entity 'primers' named entity 'RSV' named entity 'nucleic acid' named entity 'human Coronavirus' named entity 'RSV' named entity 'H1N1' named entity 'nucleic acid' named entity 'internal control' named entity 'multiplex PCR' named entity 'RT-PCR' named entity 'signs and symptoms' named entity 'HRV'

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