About: Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections

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About: Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections
Creator	Chen, Ya Cheng, Li, Dong, Dong, Pan Du, Ya Pang, Li Rong, Rong, Hu Song, Jing Xie, Guang Xiong, Zhao Jun, Duan
Source	Elsevier; Medline; PMC
abstract	Abstract Objective Newly identified human rhinovirus C (HRV-C) and human bocavirus (HBoV) cannot propagate in vitro in traditional cell culture models; thus obtaining knowledge about these viruses and developing related vaccines are difficult. Therefore, it is necessary to develop a novel platform for the propagation of these types of viruses. Methods A platform for culturing human airway epithelia in a three-dimensional (3D) pattern using Matrigel as scaffold was developed. The features of 3D culture were identified by immunochemical staining and transmission electron microscopy. Nucleic acid levels of HRV-C and HBoV in 3D cells at designated time points were quantitated by real-time polymerase chain reaction (PCR). Levels of cytokines, whose secretion was induced by the viruses, were measured by ELISA. Results Properties of bronchial-like tissues, such as the expression of biomarkers CK5, ZO-1, and PCK, and the development of cilium-like protuberances indicative of the human respiration tract, were observed in 3D-cultured human airway epithelial (HAE) cultures, but not in monolayer-cultured cells. Nucleic acid levels of HRV-C and HBoV and levels of virus-induced cytokines were also measured using the 3D culture system. Conclusion Our data provide a preliminary indication that the 3D culture model of primary epithelia using a Matrigel scaffold in vitro can be used to propagate HRV-C and HBoV.
has issue date	2018-02-28(xsd:dateTime)
bibo:doi	10.3967/bes2018.016
bibo:pmid	29606192
has license	els-covid
sha1sum (hex)	01e6b95b17fad557a310e455f5b5994628267452
schema:url	https://doi.org/10.3967/bes2018.016
resource representing a document's title	Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections
has PubMed Central identifier	PMC7134816
has PubMed identifier	29606192
schema:publication	Biomedical and Environmental Sciences
resource representing a document's body	covid:01e6b95b17fad557a310e455f5b5994628267452#body_text
is schema:about of	named entity 'Human' named entity 'HUMAN BOCAVIRUS' named entity 'OBJECTIVE' named entity 'RELATED' named entity 'HUMAN RHINOVIRUS C' named entity 'TYPES' named entity 'PROPAGATION' named entity 'VACCINES' named entity 'VIRUSES' named entity 'HUMAN RHINOVIRUS C' named entity 'INFECTIONS' named entity 'THREE-DIMENSIONAL' named entity 'HUMAN' named entity 'AIRWAY EPITHELIUM' named entity 'ORIGINAL' named entity 'ARTICLE' named entity 'CULTURE' named entity 'NECESSARY' named entity 'TRADITIONAL' named entity 'CELL CULTURE MODELS' named entity 'DIFFICULT' named entity 'NOVEL' named entity 'IN VITRO' named entity 'ABOUT' named entity 'IDENTIFIED' named entity 'NEWLY' named entity 'PLATFORM' named entity 'MATRIGEL' named entity 'KNOWLEDGE' named entity 'THESE' named entity 'BOCAVIRUS' named entity 'EVALUATION' named entity 'DEVELOPING' named entity 'propagate' named entity 'Therefore' named entity 'influenza' named entity 'phase-contrast microscope' named entity 'viruses' named entity 'culture medium' named entity 'Microplate Reader' named entity 'TEM' named entity 'cell culture' named entity 'developing' named entity 'identified' named entity 'Three-dimensional' named entity 'virus' named entity 'PCR amplification' named entity 'plasmids' named entity 'immune response' named entity 'data analysis' named entity 'Matrigel' named entity 'respiratory syncytial virus' named entity 'infection' named entity 'nucleotides' named entity 'epithelial tissues' named entity 'fluorescence microscope' named entity 'nasopharyngeal' named entity 'epitopes' named entity 'RNA' named entity 'IL-8' named entity 'PBS' named entity 'Cytokines' named entity 'antiviral' named entity 'epithelial' named entity 'viruses' named entity 'HRV' named entity 'Matrigel' named entity 'virus' named entity 'DNA virus'

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