About: Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides

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About: Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides
Creator	Chailapakul, Orawon Henry, Charles Siangproh, Weena Teengam, Prinjaporn Tuantranont, Adisorn Vilaivan, Tirayut
Source	Medline; PMC
abstract	[Image: see text] The development of simple fluorescent and colorimetric assays that enable point-of-care DNA and RNA detection has been a topic of significant research because of the utility of such assays in resource limited settings. The most common motifs utilize hybridization to a complementary detection strand coupled with a sensitive reporter molecule. Here, a paper-based colorimetric assay for DNA detection based on pyrrolidinyl peptide nucleic acid (acpcPNA)-induced nanoparticle aggregation is reported as an alternative to traditional colorimetric approaches. PNA probes are an attractive alternative to DNA and RNA probes because they are chemically and biologically stable, easily synthesized, and hybridize efficiently with the complementary DNA strands. The acpcPNA probe contains a single positive charge from the lysine at C-terminus and causes aggregation of citrate anion-stabilized silver nanoparticles (AgNPs) in the absence of complementary DNA. In the presence of target DNA, formation of the anionic DNA-acpcPNA duplex results in dispersion of the AgNPs as a result of electrostatic repulsion, giving rise to a detectable color change. Factors affecting the sensitivity and selectivity of this assay were investigated, including ionic strength, AgNP concentration, PNA concentration, and DNA strand mismatches. The method was used for screening of synthetic Middle East respiratory syndrome coronavirus (MERS-CoV), Mycobacterium tuberculosis (MTB), and human papillomavirus (HPV) DNA based on a colorimetric paper-based analytical device developed using the aforementioned principle. The oligonucleotide targets were detected by measuring the color change of AgNPs, giving detection limits of 1.53 (MERS-CoV), 1.27 (MTB), and 1.03 nM (HPV). The acpcPNA probe exhibited high selectivity for the complementary oligonucleotides over single-base-mismatch, two-base-mismatch, and noncomplementary DNA targets. The proposed paper-based colorimetric DNA sensor has potential to be an alternative approach for simple, rapid, sensitive, and selective DNA detection.
has issue date	2017-04-10(xsd:dateTime)
bibo:doi	10.1021/acs.analchem.7b00255
bibo:pmid	28394582
has license	bronze-oa
sha1sum (hex)	011c7c4ce9ad8c20533be1791a4ff09e3c536532
schema:url	https://doi.org/10.1021/acs.analchem.7b00255
resource representing a document's title	Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides
has PubMed Central identifier	PMC7077925
has PubMed identifier	28394582
schema:publication	Analytical Chemistry
resource representing a document's body	covid:011c7c4ce9ad8c20533be1791a4ff09e3c536532#body_text
is schema:about of	named entity 'rapid' named entity 'selective' named entity 'investigated' named entity 'assays' named entity 'chemically' named entity 'citrate' named entity 'complementary DNA' named entity 'based' named entity 'simple' named entity 'detection' named entity 'PAPER' named entity 'DETECTING' named entity 'MERS-COV' named entity 'PROPOSED' named entity 'MEASURING' named entity 'REPORTED' named entity 'COUPLED' named entity 'NANOPARTICLE' named entity 'SENSITIVITY' named entity 'CONTAINS' named entity 'DEVELOPMENT' named entity 'USING' named entity 'PRINCIPLE' named entity '1.53' named entity 'SIMPLE' named entity 'DNA' named entity 'SIGNIFICANT' named entity 'SINGLE' named entity 'SYNTHESIZED' named entity 'RAPID' named entity 'UTILITY' named entity 'AGGREGATION' named entity 'APPROACH' named entity 'PNA' named entity 'PROBES' named entity 'POTENTIAL' named entity 'HIGH' named entity 'SYNTHETIC' named entity 'PEPTIDE NUCLEIC ACID' named entity 'BIOLOGICALLY' named entity 'CAUSES' named entity 'DETECTABLE' named entity 'RISE' named entity 'ELECTROSTATIC' named entity 'AFFECTING' named entity 'STABLE' named entity 'CONCENTRATION' named entity 'MOLECULE' named entity 'COLOR CHANGE' named entity 'OLIGONUCLEOTIDE' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'RNA PROBES' named entity 'HYBRIDIZATION' named entity 'DNA SENSOR' named entity 'DISPERSION' named entity 'HERE' named entity 'SELECTIVITY' named entity 'REPULSION' named entity 'BASED' named entity 'REPORTER' named entity 'COLORIMETRIC ASSAY' named entity 'PRESENCE OF' named entity 'EASILY' named entity 'STABILIZED' named entity 'PROBE' named entity 'DETECTION' named entity 'POINT-OF-CARE' named entity 'MULTIPLEX' named entity 'ANALYTICAL' named entity 'SELECTIVE' named entity 'LYSINE' named entity 'CHEMICALLY' named entity 'APPROACHES' named entity 'LIMITED' named entity 'TRADITIONAL' named entity 'IONIC STRENGTH'

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