About: Multicenter comparison of the Cobas 6800 system with the RealStar RT-PCR kit for the detection of SARS-CoV-2

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About: Multicenter comparison of the Cobas 6800 system with the RealStar RT-PCR kit for the detection of SARS-CoV-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Multicenter comparison of the Cobas 6800 system with the RealStar RT-PCR kit for the detection of SARS-CoV-2
Creator	Descamps, Diane Visseaux, Benoit Legoff, Jérôme Delaugerre, Constance Le Hingrat, Quentin Boutolleau, David Feghoul, Linda Abdi, Basma Jary, Aude Wirden, Marc Bertine, Mélanie Chaix, Ferre, Marcelin, Anne-Genevieve Marie, Valentine Nere, Marie-Laure
Source	BioRxiv
abstract	Background RT-PCR testing is crucial in the diagnostic of SARS-CoV-2 infection. The use of reliable and comparable PCR assays is a cornerstone to allow use of different PCR assays depending on the local equipment. In this work, we provide a comparison of the Cobas® (Roche) and the RealStar® assay (Altona). Methods Assessment of the two assays was performed prospectively in three reference Parisians hospitals, using 170 clinical samples. They were tested with the Cobas® assay, selected to obtain a distribution of cycle threshold (Ct) as large as possible, and tested with the RealStar assay with three largely available extraction platforms: QIAsymphony (Qiagen), MagNAPure (Roche) and NucliSENS-easyMag (BioMérieux). Results Overall, the agreement (positive for at least one gene) was 76%. This rate differed considerably depending on the Cobas Ct values for gene E: below 35 (n = 91), the concordance was 99%. Regarding the positive Ct values, linear regression analysis showed a determination correlation (R2) of 0.88 and the Deming regression line revealed a strong correlation with a slope of 1.023 and an intercept of -3.9. Bland-Altman analysis showed that the mean difference (Cobas® minus RealStar®) was + 3.3 Ct, with a SD of + 2.3 Ct. Conclusions In this comparison, both RealStar® and Cobas® assays provided comparable qualitative results and a high correlation when both tests were positive. Discrepancies exist after 35 Ct and varied depending on the extraction system used for the RealStar® assay, probably due to a low viral load close to the detection limit of both assays.
has issue date	2020-06-30(xsd:dateTime)
bibo:doi	10.1101/2020.06.29.179184
has license	biorxiv
sha1sum (hex)	00cca7a85be08651a10a7575bfe055a4882b9556
schema:url	https://doi.org/10.1101/2020.06.29.179184
resource representing a document's title	Multicenter comparison of the Cobas 6800 system with the RealStar RT-PCR kit for the detection of SARS-CoV-2
schema:publication	bioRxiv
resource representing a document's body	covid:00cca7a85be08651a10a7575bfe055a4882b9556#body_text
is schema:about of	named entity 'PCR' named entity 'SARS-CoV-2' named entity 'infection' named entity 'system' named entity 'assay' named entity 'SARS-CoV-2' named entity 'infection' named entity 'Multicenter' named entity '3.5' named entity 'gene' named entity 'ORF1' named entity 'Saint Louis hospital' named entity 'Cobas' named entity 'SARS-CoV-2' named entity 'La Salpêtrière' named entity 'lysis buffer' named entity 'Bichat' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'process control' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'follow-up' named entity 'Cobas' named entity 'Germany' named entity 'gene' named entity 'RNA' named entity 'protein' named entity 'hospital laboratories' named entity 'PCR amplification' named entity 'gene' named entity 'gene' named entity 'April 2020' named entity 'RT-PCR' named entity 'gene' named entity 'RT-PCR' named entity 'Cobas' named entity 'reagent' named entity 'Covid 19' named entity 'Cobas' named entity 'SARS-CoV-2' named entity 'gene' named entity 'detection limit' named entity 'nasopharyngeal swab' named entity 'Mannheim' named entity 'SARS-CoV-2' named entity 'internal control' named entity 'Altona' named entity 'SARS-CoV-2' named entity 'gene' named entity 'Paris' named entity 'virus' named entity 'Roche' named entity 'RNA extraction' named entity 'virological' named entity 'France' named entity 'Cobas' named entity 'SARS-CoV-2' named entity 'sarbecovirus' named entity 'epidemic' named entity 'PCR' named entity 'RT-PCR' named entity 'Roche' named entity 'SARS-CoV-2' named entity 'PROVIDE' named entity 'EQUIPMENT' named entity 'ALLOW' named entity 'RELIABLE' named entity 'DIFFERENT'

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