About: A pilot study—genetic diversity and population structure of snow leopards of Gilgit-Baltistan, Pakistan, using molecular techniques

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About: A pilot study—genetic diversity and population structure of snow leopards of Gilgit-Baltistan, Pakistan, using molecular techniques Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A pilot study—genetic diversity and population structure of snow leopards of Gilgit-Baltistan, Pakistan, using molecular techniques
Creator	Aruge, Samreen Batool, Hafsa Janjua, Safia Khan, Fida
Source	PMC
abstract	BACKGROUND: The Hindu Kush and Karakoram mountain ranges in Pakistan’s northern areas are a natural habitat of the snow leopard (Panthera uncia syn. Uncia uncia) but the ecological studies on this animal are scarce since it is human shy by nature and lives in difficult mountainous tracts. The pilot study is conducted to exploit the genetic diversity and population structure of the snow leopard in this selected natural habitat of the member of the wildcat family in Pakistan. METHOD: About 50 putative scat samples of snow leopard from five localities of Gilgit-Baltistan (Pakistan) along with a control sample of zoo maintained male snow leopard were collected for comparison. Significant quality and quantity of genomic DNA was extracted from scat samples using combined Zhang–phenol–chloroform method and successful amplification of cytochrome c oxidase I gene (190 bp) using mini-barcode primers, seven simple sequence repeats (SSR) markers and Y-linked AMELY gene (200 bp) was done. RESULTS: Cytochrome c oxidase I gene sequencing suggested that 33/50 (66%) scat samples were of snow leopard. AMELY primer suggested that out of 33 amplified samples, 21 (63.63%) scats were from male and 12 (36.36%) from female leopards. Through successful amplification of DNA of 25 out of 33 (75.75%) scat samples using SSR markers, a total of 68 alleles on seven SSR loci were identified, showing low heterozygosity, while high gene flow between population. DISCUSSION: The low gene flow rate among the population results in low genetic diversity causing decreased diversification. This affects the adaptability to climatic changes, thus ultimately resulting in decreased population size of the species.
has issue date	2019-11-04(xsd:dateTime)
bibo:doi	10.7717/peerj.7672
bibo:pmid	31720096
has license	cc-by
sha1sum (hex)	008d980cbcc283a9b707de3d9a02573dde8528ac
schema:url	https://doi.org/10.7717/peerj.7672
resource representing a document's title	A pilot study—genetic diversity and population structure of snow leopards of Gilgit-Baltistan, Pakistan, using molecular techniques
has PubMed Central identifier	PMC6836756
has PubMed identifier	31720096
schema:publication	PeerJ
resource representing a document's body	covid:008d980cbcc283a9b707de3d9a02573dde8528ac#body_text
is schema:about of	named entity 'flow rate' named entity 'female' named entity 'oxidase' named entity 'diversification' named entity 'gene' named entity 'flow' named entity 'Hindu Kush' named entity 'genetic diversity' named entity 'NATURAL HABITAT' named entity 'TECHNIQUES' named entity 'USING' named entity 'SNOW' named entity 'CYTOCHROME C OXIDASE I' named entity 'QUANTITY' named entity 'AREAS' named entity 'AMPLIFIED' named entity 'DISCUSSION' named entity 'TRACTS' named entity 'BUT' named entity 'DIFFICULT' named entity 'SHY' named entity 'SIGNIFICANT' named entity 'ABOUT' named entity 'GENE SEQUENCING' named entity 'CAUSING' named entity 'COLLECTED' named entity 'SCAT' named entity '27S' named entity 'MAINTAINED' named entity 'MEMBER OF' named entity 'HETEROZYGOSITY' named entity 'PAKISTAN' named entity 'EXPLOIT' named entity 'SCATS' named entity 'ADAPTABILITY' named entity 'LEOPARDS' named entity 'GENETIC DIVERSITY' named entity 'PILOT STUDY' named entity 'GILGIT' named entity 'POPULATION STRUCTURE' named entity 'COMPARISON' named entity 'BACKGROUND' named entity 'FLOW RATE' named entity 'PILOT STUDY ' named entity 'TOTAL' named entity 'LOW' named entity 'Y-LINKED' named entity 'PAKISTAN' named entity 'WILDCAT' named entity 'ECOLOGICAL STUDIES' named entity 'GENE FLOW' named entity 'SSR' named entity 'GENE' named entity 'MARKERS' named entity 'ANIMAL' named entity 'LOCI' named entity 'RESULTS' named entity 'SUCCESSFUL' named entity 'SYN' named entity 'ZHANG' named entity 'LIVES' named entity 'GENETIC DIVERSITY' named entity 'SAMPLES' named entity 'POPULATION SIZE' named entity 'EXTRACTED' named entity 'MINI' named entity 'SSR MARKERS' named entity 'HIGH' named entity 'POPULATION STRUCTURE' named entity 'AMELY' named entity 'DECREASED' named entity 'PRIMER' named entity 'POPULATION' named entity 'AMPLIFICATION' named entity 'GENOMIC DNA' named entity 'HUMAN' named entity 'IDENTIFIED' named entity 'FEMALE'

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