About: Demonstration of the presence of a specific interferon-γ receptor on murine astrocyte cell surface

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About: Demonstration of the presence of a specific interferon-γ receptor on murine astrocyte cell surface Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Demonstration of the presence of a specific interferon-γ receptor on murine astrocyte cell surface
Creator	Rubio, Nazario De Felipe, Carmen
Source	Elsevier; Medline; PMC
abstract	Abstract Interferon gamma (IFN-γ) is a pleiotropic lymphokine produced by T-lymphocytes which acts as a soluble mediator in immunological reactions. In addition to several immune target cells, such as monocytes and macrophages, it acts on the principal glial population, the astrocytes, inducing Ia antigen expression. We have developed a binding assay for 125I-labeled recombinant murine IFN-γ, and show that, using this assay, IFN-γ interacts with a single specific receptor on the murine astrocyte cell membrane. The binding is specific and saturable and it takes place with a K d = 1.64 × 10−9 M, with 11,100 receptor molecules per astrocytic cell. The binding shows, as for macrophages, species specificity. Using an immune assay including rabbit antibodies to IFN-γ and 125I-labeled protein A, we have demonstrated an internalization of the ligand. This is an energy-dependent process, as around 50% of the bound IFN-γ is endocytosed after 4 h at 37°C when cultures are maintained in complete culture medium.
has issue date	1991-12-31(xsd:dateTime)
bibo:doi	10.1016/0165-5728(91)90166-5
bibo:pmid	1835463
has license	els-covid
sha1sum (hex)	fae10fe8d73478789a216595609f21e9735d310e
schema:url	https://doi.org/10.1016/0165-5728%2891%2990166-5
resource representing a document's title	Demonstration of the presence of a specific interferon-γ receptor on murine astrocyte cell surface
has PubMed Central identifier	PMC7119618
has PubMed identifier	1835463
schema:publication	Journal of Neuroimmunology
resource representing a document's body	covid:fae10fe8d73478789a216595609f21e9735d310e#body_text
is schema:about of	named entity 'IS A' named entity 'TARGET CELLS' named entity 'IMMUNE' named entity 'EXPRESSION' named entity 'INTERFERON GAMMA ' named entity 'IA ANTIGEN' named entity 'MEDIATOR' named entity 'GLIAL' named entity 'CELL SURFACE' named entity 'LYMPHOKINE' named entity 'ACTS' named entity 'SOLUBLE' named entity 'ADDITION' named entity 'MONOCYTES' covid:arg/fae10fe8d73478789a216595609f21e9735d310e named entity 'ASTROCYTE' named entity 'DEMONSTRATION' named entity 'POPULATION' named entity 'INDUCING' named entity 'PRINCIPAL' named entity 'ASTROCYTES' named entity 'T-LYMPHOCYTES' named entity 'REACTIONS' named entity 'IMMUNOLOGICAL' named entity 'INTERFERON' named entity 'MACROPHAGES' named entity 'SPECIFIC' named entity 'MURINE' named entity 'PRESENCE OF' named entity 'macrophages' named entity 'astrocyte' named entity 'cell surface' named entity 'molecular weight' named entity '16 mm' named entity 'astrocytes' named entity 'macrophages' named entity 'antibody' named entity 'pmol' named entity 'MBP' named entity 'astrocyte' named entity 'rat' named entity 'mice' named entity 'Biotechnology' named entity 'Richmond, CA' named entity 'cell morphology' named entity 'trichloroacetic acid' named entity 'phosphate-buffered saline' named entity 'gene' named entity 'monocytes' named entity 'glycine' named entity 'DMEM' named entity 'astrocytes' named entity 'astrocytes' named entity 'gamma counter' named entity 'glial cells' named entity 'LIGAND' named entity 'class II major histocompatibility' named entity '125I' named entity 'astrocytes' named entity 'incubation period' named entity 'PBS' named entity 'cell receptor' named entity 'Recombinant' named entity 'intracellular' named entity 'lymphokine' named entity 'fetal calf serum' named entity 'astrocytes' named entity 'IFN' named entity 'cell surface receptor' named entity 'myelin basic protein' named entity 'PBS' named entity 'rat' named entity 'astrocytes' named entity 'cell surface receptor' named entity 'Bristol-Myers' named entity 'macrophages' named entity 'molecule' named entity '37°C' named entity 'IFN' named entity 'gentamicin'

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