About: Cellular Poly(C) Binding Protein 2 Interacts with porcine epidemic diarrhea virus Papain-Like Protease 1 and Supports Viral Replication

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About: Cellular Poly(C) Binding Protein 2 Interacts with porcine epidemic diarrhea virus Papain-Like Protease 1 and Supports Viral Replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Cellular Poly(C) Binding Protein 2 Interacts with porcine epidemic diarrhea virus Papain-Like Protease 1 and Supports Viral Replication
Creator	Song, Changxu Wang, Lei Chen, Bin Huang, Li Liang, Zhang Liu, Dong Song, Chang-Xu Dong, Jianguo Zhang, Pengfei Liang, Pengshuai Liu, Yanling Yu, Linyang Zhang, Leyi
source	Elsevier; Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV) belongs to the Alphacoronavirus genus in the Coronaviridae family. Similar to other coronaviruses, PEDV encodes two papain-like proteases. Papain-like protease (PLP)2 has been proposed to play a key role in antagonizing host innate immunity. However, the function of PLP1 remains unclear. In this study, we found that overexpression of PLP1 significantly promoted PEDV replication and inhibited production of interferon-β. Immunoprecipitation and mass spectrometry were used to identify cellular interaction partners of PLP1. Host cell poly(C) binding protein 2 (PCBP2) was determined to bind and interact with PLP1. Both endogenous and overexpressed PCBP2 co-localized with PLP1 in the cytoplasm. Overexpression of PLP1 upregulated expression of PCBP2. Furthermore, overexpression of PCBP2 promoted PEDV replication. Silencing of endogenous PCBP2 using small interfering RNAs attenuated PEDV replication. Taken together, these data demonstrated that PLP1 negatively regulated the production of type 1 interferon by interacting with PCBP2 and promoted PEDV replication.
has issue date	2020-07-13(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2020.108793
bibo:pmid	32768236
has license	no-cc
sha1sum (hex)	f22a0a0e2a43e08a44982197ee33fa24006f641e
schema:url	https://doi.org/10.1016/j.vetmic.2020.108793
resource representing a document's title	Cellular Poly(C) Binding Protein 2 Interacts with porcine epidemic diarrhea virus Papain-Like Protease 1 and Supports Viral Replication
has PubMed Central identifier	PMC7355335
has PubMed identifier	32768236
schema:publication	Vet Microbiol
resource representing a document's body	covid:f22a0a0e2a43e08a44982197ee33fa24006f641e#body_text
is schema:about of	named entity 'Viral' named entity 'Protease' named entity 'IFN' named entity 'INDUCED' named entity 'EXPRESSION' named entity 'interacts' named entity 'TNF-α' named entity 'TNF-α' named entity 'IFN' named entity 'PLP1' named entity 'Papain' named entity 'Protease' named entity 'Papain' named entity 'Protease' named entity 'type I IFN' named entity 'LC-MS/MS' named entity 'Silver staining' named entity 'RIG-I' named entity 'dose-dependent manner' named entity 'Co-IP' named entity 'internal control' named entity 'Vero E6' named entity 'Thermo Scientific' named entity 'Nsp3' named entity 'transfection' named entity 'Lipofectamine' named entity 'PCBP2' named entity 'PLP1' named entity 'PLP1' named entity 'PEDV' named entity 'HEK293T cells' named entity 'polyclonal' named entity 'antibody' named entity 'PLP1' named entity 'Triton X-100' named entity 'eluent' named entity 'PEDV' named entity 'PLP1' named entity 'PEDV' named entity 'anti-FLAG' named entity 'PCBP2' named entity 'NF-κB' named entity 'PLP1' named entity 'IFN' named entity 'PLP1' named entity 'porcine' named entity 'nitrocellulose' named entity 'vector' named entity 'electrophoresed' named entity 'receptor' named entity 'endogenous' named entity 'Danvers, MA' named entity 'PLP1' named entity 'leupeptin' named entity 'RNA extraction' named entity 'NSP' named entity 'PLP1' named entity 'Japan' named entity 'siRNA' named entity 'PLP1' named entity 'Cell Signaling Technology' named entity 'PCBP2' named entity 'PLP1' named entity 'PLP1' named entity 'PEDV' named entity 'cytoplasm' named entity 'upregulation' named entity 'transcription factor' named entity 'vector'

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