About: Cryo X-ray nano-tomography of vaccinia virus infected cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cryo X-ray nano-tomography of vaccinia virus infected cells
Creator	Chiappi, Michele Esteban, Mariano Guttmann, Peter Perdiguero, Beatriz Pereiro, Eva Rehbein, Stefan Schneider, Gerd Werner, Stephan Carrascosa, José Chichón, Francisco Rodríguez, Maria
Source	Elsevier; Medline; PMC
abstract	We have performed full-field cryo X-ray microscopy in the water window photon energy range on vaccinia virus (VACV) infected cells to produce tomographic reconstructions. PtK2 cells were infected with a GFP-expressing VACV strain and frozen by plunge fast freezing. The infected cells were selected by light fluorescence microscopy of the GFP marker and subsequently imaged in the X-ray microscope under cryogenic conditions. Tomographic tilt series of X-ray images were used to yield three-dimensional reconstructions showing different cell organelles (nuclei, mitochondria, filaments), together with other structures derived from the virus infection. Among them, it was possible to detect viral factories and two types of viral particles related to different maturation steps of VACV (immature and mature particles), which were compared to images obtained by standard electron microscopy of the same type of cells. In addition, the effect of radiation damage during X-ray tomographic acquisition was analyzed. Thin sections studied by electron microscopy revealed that the morphological features of the cells do not present noticeable changes after irradiation. Our findings show that cryo X-ray nano-tomography is a powerful tool for collecting three-dimensional structural information from frozen, unfixed, unstained whole cells with sufficient resolution to detect different virus particles exhibiting distinct maturation levels.
has issue date	2011-12-08(xsd:dateTime)
bibo:doi	10.1016/j.jsb.2011.12.001
bibo:pmid	22178221
has license	no-cc
sha1sum (hex)	f0e81bd1111037db25cbfabb9ba2722ae340ea32
schema:url	https://doi.org/10.1016/j.jsb.2011.12.001
resource representing a document's title	Cryo X-ray nano-tomography of vaccinia virus infected cells
has PubMed Central identifier	PMC7119024
has PubMed identifier	22178221
schema:publication	J Struct Biol
resource representing a document's body	covid:f0e81bd1111037db25cbfabb9ba2722ae340ea32#body_text
is schema:about of	named entity 'FAST' named entity 'DIFFERENT' named entity 'TO DETECT' named entity 'CRYOGENIC' named entity 'OBTAINED BY' named entity 'FEATURES' named entity 'OTHER STRUCTURES' named entity 'ANALYZED' named entity 'TOOL' named entity 'CELL ORGANELLES' named entity 'INFECTED' named entity 'IMAGED' named entity 'MATURATION' named entity 'USED' named entity 'STANDARD' named entity 'INFORMATION' named entity 'TILT' named entity 'FREEZING' named entity 'conditions' named entity 'infected' named entity 'Our' named entity 'IMAGES' named entity 'FILAMENTS' named entity 'FACTORIES' named entity 'PTK2' named entity 'WATER' named entity 'MITOCHONDRIA' named entity 'SUFFICIENT' named entity 'ELECTRON MICROSCOPY' named entity 'THREE-DIMENSIONAL' named entity 'ADDITION' named entity 'TOMOGRAPHY' named entity 'INFECTED' named entity 'VACCINIA VIRUS' named entity 'OUR' named entity 'COMPARED' named entity 'MARKER' named entity 'DERIVED' named entity 'SAME TYPE' named entity 'TYPES' named entity 'VIRAL' named entity 'YIELD' named entity 'DISTINCT' named entity 'VIRUS INFECTION' named entity 'SERIES' named entity 'TO PRODUCE' named entity 'NOT PRESENT' named entity 'VIRAL PARTICLES' named entity 'LIGHT' named entity 'LEVELS' named entity 'CELLS' named entity 'NANO-' named entity 'X-RAY' named entity 'POWERFUL' named entity 'FROZEN' named entity 'WINDOW' named entity 'CONDITIONS' named entity 'POSSIBLE' named entity 'PARTICLES' named entity 'HAVE' named entity 'IRRADIATION' named entity 'GFP' named entity 'RADIATION DAMAGE' named entity 'RANGE' named entity 'PHOTON ENERGY' named entity 'IS A' named entity 'NANO-' named entity 'MORPHOLOGICAL' named entity 'EFFECT' named entity 'EXHIBITING' named entity 'COLLECTING'

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