About: Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS

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About: Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS
Creator	Mizutani, Tetsuya Matsuyama, Shutoku Fukushi, Shuetsu Morikawa, Shigeru Saijo, Masayuki Tashiro, Masato Kurane, Ichiro Taguchi, Fumihiro Notomi, Tsugunori Kanda, Hidetoshi Minekawa, Harumi Ogino, Toshio Hanh, Hong Long, Hoang Thi, Nguyen
Source	Elsevier; Medline; PMC
abstract	The recombinant nucleocapsid protein (rNP) of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) was expressed in a baculovirus system. The purified SARS-CoV rNP was used as an antigen for detection of SARS-CoV antibodies in IgG enzyme-linked immunosorbent assay (ELISA). The ELISA was evaluated in comparison with neutralizing antibody assay and the authentic SARS-CoV antigen-based IgG ELISA. Two-hundred and seventy-six serum samples were collected from health care workers in a hospital in which a nosocomial SARS outbreak took place and used for evaluation. The SARS-CoV rNP-based IgG ELISA has 92% of sensitivity and specificity compared with the neutralizing antibody assay and 94% sensitivity and specificity compared with the authentic SARS-CoV antigen-based IgG ELISA. The results suggest that the newly developed SARS-CoV rNP-based IgG ELISA is a valuable tool for the diagnosis and seroepidemiological study of SARS. The SARS-CoV rNP-based IgG ELISA has an advantage over the conventional IgG ELISA in that the antigen can be prepared by laboratory workers without the risk of infection.
has issue date	2005-02-24(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2005.01.028
bibo:pmid	15794988
has license	no-cc
sha1sum (hex)	f02dfef2035b19d983f4687dda7a732f68be7d87
schema:url	https://doi.org/10.1016/j.jviromet.2005.01.028
resource representing a document's title	Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS
has PubMed Central identifier	PMC7112814
has PubMed identifier	15794988
schema:publication	J Virol Methods
resource representing a document's body	covid:f02dfef2035b19d983f4687dda7a732f68be7d87#body_text
is schema:about of	named entity 'IgG' named entity 'system' named entity 'collected' named entity 'Recombinant' named entity 'OUTBREAK' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'DETECTION' named entity 'COLLECTED' named entity 'ADVANTAGE' named entity 'IGG' named entity 'PROTEIN ' named entity 'PURIFIED' named entity 'SUGGEST' named entity 'IGG' named entity 'HOSPITAL' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'BASED' named entity 'SARS' named entity 'RECOMBINANT' named entity 'NUCLEOCAPSID PROTEIN' named entity 'ANTIBODIES' named entity 'SAMPLES' named entity 'BASED' named entity 'DIAGNOSIS' named entity 'LABORATORY' named entity 'CORONAVIRUS' named entity 'USED' named entity 'CONVENTIONAL' named entity 'NUCLEOCAPSID PROTEIN' named entity 'COMPARISON' named entity 'SARS-COV' named entity 'NEWLY' named entity 'SEROLOGICAL DIAGNOSIS' named entity 'TOOL' named entity 'SEVENTY' named entity 'EVALUATION' named entity 'TOOK PLACE' named entity 'SARS' named entity 'ANTIGEN' named entity 'SYSTEM' named entity 'RECOMBINANT' named entity 'ASSAY' named entity 'RESULTS' named entity 'HEALTH CARE' named entity 'NEUTRALIZING ANTIBODY' named entity 'BACULOVIRUS' named entity 'SERUM' named entity 'RISK OF INFECTION' named entity 'PREPARED' named entity 'SEROEPIDEMIOLOGICAL STUDY' named entity 'EVALUATED' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'IS A' named entity 'ELISA' named entity 'RNP' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'WORKERS' named entity 'EXPRESSED' covid:arg/f02dfef2035b19d983f4687dda7a732f68be7d87 named entity 'purified' named entity 'The' named entity 'SARS-CoV' named entity 'recombinant' named entity 'SARS-CoV' named entity 'nosocomial' named entity 'SARS outbreak' named entity 'IgG'

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