About: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs
Creator	Poovorawan, Yong Piewbang, Chutchai Techangamsuwan, Somporn Rungsipipat, Anudep
Source	Medline; PMC
abstract	Canine infectious respiratory disease complex (CIRDC) viruses have been detected in dogs with respiratory illness. Canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine distemper virus (CDV), canine respiratory coronavirus (CRCoV), canine adenovirus type 2 (CAdV-2) and canine herpesvirus 1 (CaHV-1), are all associated with the CIRDC. To allow diagnosis, two conventional multiplex polymerase chain reactions (PCR) were developed to simultaneously identify four RNA and two DNA viruses associated with CIRDC. The two multiplex PCR assays were then validated on 102 respiratory samples collected from 51 dogs with respiratory illness by sensitivity and specificity determination in comparison to conventional simplex PCR and a rapid three-antigen test kit. All six viruses were detected in either individual or multiple infections. The developed multiplex PCR assays had a >87% sensitivity and 100% specificity compared to their simplex counterpart. Compared to the three-antigen test kit, the multiplex PCR assays yielded 100% sensitivity and more than 83% specificity for detection of CAdV-2 and CDV, but not for CIV. Therefore, the developed multiplex PCR modalities were able to simultaneously diagnose a panel of CIRDC viruses and facilitated specimen collection through being suitable for use of nasal or oral samples.
has issue date	2016-09-15(xsd:dateTime)
bibo:doi	10.1292/jvms.16-0342
bibo:pmid	27628592
has license	cc-by-nc-nd
sha1sum (hex)	ef6dbb94ddc618e006e993f32893043b9fa87463
schema:url	https://doi.org/10.1292/jvms.16-0342
resource representing a document's title	Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs
has PubMed Central identifier	PMC5240764
has PubMed identifier	27628592
schema:publication	J Vet Med Sci
resource representing a document's body	covid:ef6dbb94ddc618e006e993f32893043b9fa87463#body_text
is schema:about of	named entity 'PAPER' named entity 'CONVENTIONAL' named entity 'THEIR' named entity 'POLYMERASE CHAIN REACTIONS' named entity 'MORE THAN' named entity 'CIV' named entity 'SIMULTANEOUSLY' named entity 'COMPLEX' named entity 'RAPID' named entity 'PANEL' named entity 'ALLOW' named entity 'CANINE INFLUENZA VIRUS' named entity 'DIAGNOSIS' named entity 'SPECIFICITY' named entity 'CANINE HERPESVIRUS 1' named entity 'DIAGNOSE' named entity 'COLLECTED' named entity 'sensitivity' named entity 'complex' named entity 'simplex' named entity 'CDV' named entity 'conventional' named entity 'sensitivity' named entity 'multiplex PCR' named entity 'PAPER' named entity 'application' named entity 'DEVELOPMENT' named entity 'THAILAND' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'SUITABLE' named entity 'DOGS' named entity 'ANTIGEN TEST' named entity 'INDIVIDUAL' named entity 'MULTIPLE INFECTIONS' named entity 'RNA' named entity 'MULTIPLEX' named entity 'CDV' named entity 'RESPIRATORY SAMPLES' named entity '102' named entity 'HAVE' named entity '100%' named entity 'VIROLOGY' named entity 'INDUCED' named entity 'MULTIPLEX PCR' named entity 'DETECTION OF VIRUS' named entity 'COMPLEX' named entity 'APPLICATION' named entity 'INFECTIOUS' named entity 'MULTIPLEX PCR' named entity 'DNA VIRUSES' named entity 'IDENTIFY' named entity 'COMPARISON' named entity 'BEING' named entity 'RESPIRATORY ILLNESS' named entity 'ASSOCIATED WITH' named entity 'ORAL' named entity 'CANINE PARAINFLUENZA VIRUS' named entity 'WORDS' named entity 'RESPIRATORY DISEASE' named entity 'DETERMINATION' named entity 'VIRUSES' named entity 'USE OF' named entity 'DETECTION' named entity 'TEST KIT' named entity 'CANINE' named entity 'SENSITIVITY' named entity 'CANINE DISTEMPER VIRUS' named entity 'BUT' named entity 'RESPIRATORY DISEASE' named entity 'MODALITIES' named entity 'CANINE RESPIRATORY CORONAVIRUS' named entity 'DOGS' named entity 'VALIDATED' named entity 'NASAL' named entity 'SAMPLES' named entity 'EITHER' named entity 'CANINE ADENOVIRUS TYPE 2' named entity 'KEY'

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