About: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain

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About: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain
Creator	Huang, Mei Zhong, Yanxin Gao, Yuan Guo, Huichen Sun, Shiqi Wei, Yanquan Yuan, Quan Gu, Lingjun Liu, Dingxiang Mao, Lejiao Tan, Yong
Source	Medline; PMC
abstract	Lipid raft is an important element for the cellular entry of some viruses, including coronavirus infectious bronchitis virus (IBV). However, the exact role of lipid rafts in the cellular membrane during the entry of IBV into host cells is still unknown. In this study, we biochemically fractionated IBV-infected cells via sucrose density gradient centrifugation after depleting plasma membrane cholesterol with methyl-β-cyclodextrin or Mevastatin. Our results demonstrated that unlike IBV non-structural proteins, IBV structural proteins co-localized with lipid raft marker caveolin-1. Infectivity assay results of Vero cells illustrated that the drug-induced disruption of lipid rafts significantly suppressed IBV infection. Further studies revealed that lipid rafts were not required for IBV genome replication or virion release at later stages. However, the drug-mediated depletion of lipid rafts in Vero cells before IBV attachment significantly reduced the expression of viral structural proteins, suggesting that drug treatment impaired the attachment of IBV to the cell surface. Our results indicated that lipid rafts serve as attachment factors during the early stages of IBV infection, especially during the attachment stage.
has issue date	2017-01-12(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0170123
bibo:pmid	28081264
has license	cc-by
sha1sum (hex)	ebbaea6d90c8fe4ccbb6e65a842689b104acba90
schema:url	https://doi.org/10.1371/journal.pone.0170123
resource representing a document's title	The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain
has PubMed Central identifier	PMC5231368
has PubMed identifier	28081264
schema:publication	PLoS One
resource representing a document's body	covid:ebbaea6d90c8fe4ccbb6e65a842689b104acba90#body_text
is schema:about of	named entity 'ATTACHMENT' named entity 'Mevastatin' named entity 'CORONAVIRUS' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'STUDIES' named entity 'INFECTION' named entity 'MARKER' named entity 'STRAIN' named entity 'INCLUDING' named entity 'RELEASE' named entity 'ELEMENT' named entity 'OUR' named entity 'DEPLETING' named entity 'STRUCTURAL PROTEINS' named entity 'CYCLODEXTRIN' named entity 'HOST CELLS' named entity 'INDICATED' named entity 'STAGES' named entity 'SUCROSE DENSITY GRADIENT CENTRIFUGATION' named entity 'CHOLESTEROL' named entity 'DRUG TREATMENT' named entity 'NON-STRUCTURAL PROTEINS' named entity 'LIPID RAFT' named entity 'ROLE' named entity 'IMPORTANT' named entity 'VIRUSES' named entity 'CELL SURFACE' named entity 'METHYL' named entity 'STUDY' named entity 'MEVASTATIN' named entity 'FRACTIONATED' named entity 'CULTURED CELLS' named entity 'MEDIATED' named entity 'INFECTED' named entity 'ATTACHMENT' named entity 'REVEALED' named entity 'DEPLETION' named entity 'ASSAY' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'INFECTION' named entity 'ENTRY' named entity 'DRUG' named entity 'EXPRESSION' named entity 'STAGE' named entity 'NOT REQUIRED' named entity 'VERO CELLS' named entity 'CORONAVIRUS' named entity 'CELLULAR' named entity 'LOCALIZED' named entity 'EARLY STAGES' named entity 'INFECTIVITY' named entity 'CELLS' named entity 'IMPAIRED' named entity 'RESULTS' named entity 'CELLULAR MEMBRANE' named entity 'VIRION' named entity 'IMPORTANT' named entity 'DEMONSTRATED' named entity 'CAVEOLIN-1' named entity 'PLASMA MEMBRANE' named entity 'ROLE' named entity 'MEDIATED' named entity 'SUPPRESSED' named entity 'LIPID RAFT' named entity 'GENOME' named entity 'DISRUPTION' named entity 'DRUG-INDUCED' named entity 'REDUCED' named entity 'SERVE' named entity 'REPLICATION' named entity 'EXACT'

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