About: Liposome-Coupled Antigens Are Internalized by Antigen-Presenting Cells via Pinocytosis and Cross-Presented to CD8(+) T Cells

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About: Liposome-Coupled Antigens Are Internalized by Antigen-Presenting Cells via Pinocytosis and Cross-Presented to CD8(+) T Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Liposome-Coupled Antigens Are Internalized by Antigen-Presenting Cells via Pinocytosis and Cross-Presented to CD8(+) T Cells
Creator	Kakiuchi, Terutaka Tanaka, Yuriko Taneichi, Maiko Uchida, Tetsuya Kasai, Michiyuki
Source	PMC
abstract	We have previously demonstrated that antigens chemically coupled to the surface of liposomes consisting of unsaturated fatty acids were cross-presented by antigen-presenting cells (APCs) to CD8(+) T cells, and that this process resulted in the induction of antigen-specific cytotoxic T lymphocytes. In the present study, the mechanism by which the liposome-coupled antigens were cross-presented to CD8(+) T cells by APCs was investigated. Confocal laser scanning microscopic analysis demonstrated that antigens coupled to the surface of unsaturated-fatty-acid-based liposomes received processing at both MHC class I and class II compartments, while most of the antigens coupled to the surface of saturated-fatty-acid-based liposomes received processing at the class II compartment. In addition, flow cytometric analysis demonstrated that antigens coupled to the surface of unsaturated-fatty-acid-liposomes were taken up by APCs even in a 4°C environment; this was not true of saturated-fatty-acid-liposomes. When two kinds of inhibitors, dimethylamiloride (DMA) and cytochalasin B, which inhibit pinocytosis and phagocytosis by APCs, respectively, were added to the culture of APCs prior to the antigen pulse, DMA but not cytochalasin B significantly reduced uptake of liposome-coupled antigens. Further analysis of intracellular trafficking of liposomal antigens using confocal laser scanning microscopy revealed that a portion of liposome-coupled antigens taken up by APCs were delivered to the lysosome compartment. In agreement with the reduction of antigen uptake by APCs, antigen presentation by APCs was significantly inhibited by DMA, and resulted in the reduction of IFN-γ production by antigen-specific CD8(+) T cells. These results suggest that antigens coupled to the surface of liposomes consisting of unsaturated fatty acids might be pinocytosed by APCs, loaded onto the class I MHC processing pathway, and presented to CD8(+) T cells. Thus, these liposome-coupled antigens are expected to be applicable for the development of vaccines that induce cellular immunity.
has issue date	2010-12-17(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0015225
bibo:pmid	21179411
has license	cc-by
sha1sum (hex)	e8c2bc95762a2aefd919b02ae90131bc67716140
schema:url	https://doi.org/10.1371/journal.pone.0015225
resource representing a document's title	Liposome-Coupled Antigens Are Internalized by Antigen-Presenting Cells via Pinocytosis and Cross-Presented to CD8(+) T Cells
has PubMed Central identifier	PMC3003686
has PubMed identifier	21179411
schema:publication	PLoS One
resource representing a document's body	covid:e8c2bc95762a2aefd919b02ae90131bc67716140#body_text
is schema:about of	named entity 'CD8' named entity 'laser scanning' named entity 'DMA' named entity 'lysosome' named entity 'surface' named entity 'Pinocytosis' covid:arg/e8c2bc95762a2aefd919b02ae90131bc67716140 named entity 'MHC class I' named entity 'antigens' named entity 'APCs' named entity 'confocal laser scanning microscopy' named entity 'flow cytometric' named entity 'loaded' named entity 'T cells' named entity 'chemically' named entity 'T cells' named entity 'DMA' named entity 'APCs' named entity 'liposomal' named entity 'surface' named entity 'inhibitors' named entity 'T Cells' named entity 'CD8' named entity 'antigens' named entity 'CD8 +' named entity 'antigens' named entity 'antigens' named entity 'fatty-acid' named entity 'liposomes' named entity 'microscopic analysis' named entity 'antigen' named entity 'intracellular trafficking' named entity 'processing pathway' named entity 'flow cytometric' named entity 'phagocytosis' named entity 'Liposome' named entity 'Antigen-Presenting Cells' named entity 'Antigens' named entity 'antigens' named entity 'liposome' named entity 'CD8 +' named entity '0.45' named entity 'liposomes' named entity 'OVA' named entity 'lysosomal' named entity 'OVA' named entity 'organelles' named entity 'oleoyl' named entity 'virus' named entity 'primers' named entity 'mannose' named entity 'oleoyl' named entity 'OVA' named entity 'macrophages' named entity 'rat' named entity 'CD11c' named entity 'syringe filter' named entity 'liposomes' named entity 'confocal laser scanning microscopy' named entity 'antigen presentation' named entity 'macrophages' named entity 'OVA' named entity 'liposomes' named entity 'liposome' named entity 'liposomes' named entity 'column chromatography' named entity 'clone' named entity 'CD8 +' named entity 'CTL' named entity 'MHC class II' named entity 'CpG' named entity 'macrophages' named entity 'class II' named entity 'OVA' named entity 'GE Healthcare' named entity 'lysosomal' named entity 'Miltenyi Biotec'

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