About: Cholesterol-rich lipid rafts play an important role in the Cyprinid herpesvirus 3 replication cycle()

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About: Cholesterol-rich lipid rafts play an important role in the Cyprinid herpesvirus 3 replication cycle() Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cholesterol-rich lipid rafts play an important role in the Cyprinid herpesvirus 3 replication cycle()
Creator	Adamek, Mikołaj Brogden, Graham Steinhagen, Dieter Ulrich, Reiner Naim, Hassan Proepsting, Marcus
Source	Elsevier; Medline; PMC
abstract	The Cyprinus herpesvirus 3 (CyHV-3) is a member of the new Alloherpesviridae virus family in the Herpesvirales order. CyHV-3 has been implicated in a large number of disease outbreaks in carp populations causing up to 100% mortality. The aim of this study was to investigate the requirement of cholesterol-rich lipid rafts in CyHV-3 entry and replication in carp cells. Plasma membrane cholesterol was depleted from common carp brain (CCB) cells with methyl-β-cyclodextrin (MβCD). Treated and non-treated cells were infected with CyHV-3 and virus binding and infection parameters were assessed using RT-qPCR, immunocytochemistry and virus titration. The effect of cholesterol reduction severely stunted virus entry in vitro, however after cholesterol replenishment virus entry and subsequent replication rates were similar to the control infection. Furthermore, cholesterol depletion did not significantly influence virus binding and the subsequent post-entry replication stage, however had an impact on virus egress. Comparative analysis of the lipid compositions of CyHV-3 and CCB membrane fractions revealed strong similarities between the lipid composition of the CyHV-3 and CCB lipid rafts. The results presented here show that cholesterol-rich lipid rafts are important for the CyHV-3 replication cycle especially during entry and egress.
has issue date	2015-09-30(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2015.05.024
bibo:pmid	26059657
has license	no-cc
sha1sum (hex)	e5adf7aca5308543613d1f966616c2fbec18ec21
schema:url	https://doi.org/10.1016/j.vetmic.2015.05.024
resource representing a document's title	Cholesterol-rich lipid rafts play an important role in the Cyprinid herpesvirus 3 replication cycle()
has PubMed Central identifier	PMC7117466
has PubMed identifier	26059657
schema:publication	Vet Microbiol
resource representing a document's body	covid:e5adf7aca5308543613d1f966616c2fbec18ec21#body_text
is schema:about of	named entity 'presented' named entity 'carp' named entity 'Herpesvirales' named entity 'FRACTIONS' named entity 'VIRUS EGRESS' named entity 'MORTALITY' named entity 'CAUSING' named entity 'RATES' named entity 'DISEASE OUTBREAKS' named entity 'IN VITRO' named entity 'REPLENISHMENT' named entity 'POPULATIONS' named entity 'VIRUS ENTRY' named entity 'REPLICATION CYCLE' named entity 'RT-QPCR' named entity 'SUBSEQUENT' named entity 'UP TO' named entity 'STUDY' named entity 'IMMUNOCYTOCHEMISTRY' named entity 'PARAMETERS' named entity 'TO INVESTIGATE' named entity 'NUMBER OF' named entity 'MEMBER OF' named entity 'LIPID COMPOSITION' named entity 'ENTRY' named entity 'CARP' named entity 'VIRUS BINDING' named entity 'POST' named entity 'ALLOHERPESVIRIDAE' named entity 'RESULTS' named entity 'CELLS' named entity 'REQUIREMENT' named entity 'LIPID' named entity 'HERPESVIRALES' named entity 'CYCLODEXTRIN' named entity 'CHOLESTEROL' named entity 'IMPORTANT' named entity 'MEMBRANE' named entity 'COMMON CARP' named entity 'CHOLESTEROL' named entity 'PLAY' named entity 'REVEALED' named entity 'HERE' named entity 'SIMILAR' named entity 'NEW' named entity 'COMPARATIVE ANALYSIS' named entity 'IMPACT' named entity 'TITRATION' named entity 'INFECTED' named entity 'USING' named entity 'EFFECT' named entity 'IMPORTANT' named entity 'REPLICATION CYCLE' named entity 'STRONG' named entity 'COMPOSITIONS' named entity 'DEPLETED' named entity 'CYPRINID HERPESVIRUS 3' named entity 'LIPID RAFTS' named entity 'RICH' named entity 'CONTROL INFECTION' named entity '100%' named entity 'IS A' named entity 'STAGE' named entity 'CHOLESTEROL DEPLETION' named entity 'INFLUENCE' named entity 'INFECTION' named entity 'SEVERELY' named entity 'NONTREATED' named entity 'DID' named entity 'REDUCTION' named entity 'RICH' named entity 'METHYL' named entity 'PRESENTED' named entity 'VIRUS' named entity 'HERPESVIRUS' named entity 'ORDER'

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