About: Purification, identification and profiling of serum amyloid A proteins from sera of advanced-stage cancer patients

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Purification, identification and profiling of serum amyloid A proteins from sera of advanced-stage cancer patients
Creator	Li, Jing Cai, Tanxi Chen, Xiulan Cui, Ziyou Guo, Sutang Hou, Junjie Shi, Linan Wu, Peng Xie, Zhensheng Xue, Peng Yang, Fuquan Zhao, Zhiqiang
source	Elsevier; Medline; PMC
abstract	Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) is a powerful tool for screening potential biomarkers of various pathological conditions. However, low resolution and mass accuracy of SELDI-TOF-MS remain a major obstacle for determination of biological identities of potential protein biomarkers. We report here a refined workflow that combines ZipTip desalting, acetonitrile precipitation, high-performance liquid chromatography (HPLC) separation and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis for the profiling, purification and identification of the targeted serum proteins found by SELDI-TOF-MS. By using this workflow, we purified ten targeted proteins from the sera of patients with various types of advanced stage (stage III–IV) cancers. These proteins were identified as isoforms of the human serum amyloid protein A (SAA) family with or without truncations at their N-terminals. This was confirmed by Western blot analysis. Different SAA expression patterns were observed by MALDI-TOF-MS profiling. SAA has long been reported as a biomarker for various cancer types such as lung cancer, ovarian cancer, and breast cancer. However, in this study we found increased SAA expression in the sera of advanced-stage cancer patients with different cancer types. Our results suggest that maybe SAA should not be used alone as a biomarker for any specific cancer type.
has issue date	2012-03-15(xsd:dateTime)
bibo:doi	10.1016/j.jchromb.2012.01.002
bibo:pmid	22341353
has license	no-cc
sha1sum (hex)	e4764e0cd8dbbc32b3b34d9101299d3107c2d7a6
schema:url	https://doi.org/10.1016/j.jchromb.2012.01.002
resource representing a document's title	Purification, identification and profiling of serum amyloid A proteins from sera of advanced-stage cancer patients
has PubMed Central identifier	PMC7105184
has PubMed identifier	22341353
schema:publication	J Chromatogr B Analyt Technol Biomed Life Sci
resource representing a document's body	covid:e4764e0cd8dbbc32b3b34d9101299d3107c2d7a6#body_text
is schema:about of	named entity 'human' named entity 'remain' named entity 'protein' named entity 'patterns' named entity 'patients' named entity 'profiling' named entity 'patients' named entity 'PROTEINS' named entity 'IS A' named entity 'CONDITIONS' named entity 'PROTEINS' named entity 'PURIFICATION' named entity 'MALDI-TOF-MS' named entity 'PURIFIED' named entity 'PATHOLOGICAL' named entity 'POWERFUL' named entity 'RESULTS' named entity 'SERUM PROTEINS' named entity 'HERE' named entity 'SERA' named entity 'WORKFLOW' named entity 'MASS' named entity 'ACETONITRILE' named entity 'OUR' named entity 'ASSISTED' named entity 'SERUM AMYLOID A' named entity 'STAGE CANCER' named entity 'PURIFICATION' named entity 'PATIENTS' named entity 'ADVANCED' named entity 'IDENTIFICATION' named entity 'IDENTIFICATION' named entity 'LONG' named entity 'ANALYSIS' named entity 'ENHANCED' named entity 'DETERMINATION' named entity 'TOOL' named entity 'ISOFORMS' named entity 'SERA' named entity 'INCREASED' named entity 'EXPRESSION' named entity 'BIOMARKERS' named entity 'STAGE CANCER' named entity 'LIQUID CHROMATOGRAPHY ' named entity 'LASER DESORPTION' named entity 'SURFACE' named entity 'USING' named entity 'LOW RESOLUTION' named entity 'PROTEIN ' named entity 'A MAJOR' named entity 'USED' named entity 'THESE' named entity 'REPORTED' named entity 'CANCERS' named entity 'SELDI-TOF-MS' named entity 'CANCER TYPE' named entity 'HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY' named entity 'FAMILY' named entity 'PATTERNS' named entity 'CANCER' named entity 'THEIR' named entity 'BREAST CANCER' named entity 'TOF-MS' named entity 'WESTERN BLOT ANALYSIS' named entity 'MAYBE' named entity 'ADVANCED STAGE' named entity 'PRECIPITATION' named entity 'OBSTACLE' named entity 'IDENTITIES' named entity 'III'

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