About: Preparation of Aluminum Oxide-Coated Glass Slides for Glycan Microarrays

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About: Preparation of Aluminum Oxide-Coated Glass Slides for Glycan Microarrays Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Preparation of Aluminum Oxide-Coated Glass Slides for Glycan Microarrays
Creator	Wong, Chi-Huey Wu, Chung-Yi Chang, Shih-Huang Chiang, Donyau Cho, Wen-Hao Hsiao, Chien-Nan Su, James Tseng, Susan
Source	PMC
abstract	[Image: see text] In this study, we report the fabrication of aluminum oxide-coated glass (ACG) slides for the preparation of glycan microarrays. Pure aluminum (Al, 300 nm) was coated on glass slides via electron-beam vapor deposition polymerization (VDP), followed by anodization to form a thin layer (50–65 nm) of aluminum oxide (Al-oxide) on the surface. The ACG slides prepared this way provide a smooth surface for arraying sugars covalently via phosphonate formation with controlled density and spatial distance. To evaluate this array system, a mannose derivative of α-5-pentylphosphonic acid was used as a model for the optimization of covalent arraying based on the fluorescence response of the surface mannose interacting with concanavalin A (ConA) tagged with the fluorescence probe A488. The ACG slide was characterized using scanning electron microscopy, atomic force microscopy (AFM), and ellipsometry, and the sugar loading capacity, uniformity, and structural conformation were also characterized using AFM, a GenePix scanner, and a confocal microscope. This study has demonstrated that the glycan array prepared from the ACG slide is more homogeneous with better spatial control compared with the commonly used glycan array prepared from the N-hydroxysuccinimide-activated glass slide.
has issue date	2016-11-03(xsd:dateTime)
bibo:doi	10.1021/acsomega.6b00143
bibo:pmid	30023491
has license	no-cc
sha1sum (hex)	e03a59f8c651cd120e4c06a6c4f6e87b20d5e2d4
schema:url	https://doi.org/10.1021/acsomega.6b00143
resource representing a document's title	Preparation of Aluminum Oxide-Coated Glass Slides for Glycan Microarrays
has PubMed Central identifier	PMC6044682
has PubMed identifier	30023491
schema:publication	ACS Omega
resource representing a document's body	covid:e03a59f8c651cd120e4c06a6c4f6e87b20d5e2d4#body_text
is schema:about of	named entity 'tagged' named entity 'prepared' named entity 'commonly' named entity 'demonstrated' named entity 'glass' named entity 'scanning electron microscopy' named entity 'surface' named entity 'AFM' named entity 'PURE' named entity 'ANODIZATION' named entity 'DEMONSTRATED' named entity 'CHARACTERIZED' named entity 'PREPARATION' named entity 'SMOOTH SURFACE' named entity 'ELLIPSOMETRY' named entity 'DERIVATIVE' named entity 'COATED' named entity 'ARRAY' named entity 'ACTIVATED' named entity 'COVALENT' named entity 'CAPACITY' named entity 'CONFOCAL MICROSCOPE' named entity 'LAYER' named entity 'INTERACTING' named entity 'BETTER' named entity 'ACG' named entity 'FLUORESCENCE' named entity 'ALUMINUM OXIDE' named entity 'RESPONSE' named entity 'SCANNER' named entity 'PROVIDE' named entity 'ALUMINUM OXIDE' named entity 'COATED' named entity 'USED' named entity 'GLYCAN' named entity 'MICROARRAYS' named entity 'HOMOGENEOUS' named entity 'COMPARED' named entity 'CONTROL' named entity 'WAY' named entity 'SCANNING ELECTRON MICROSCOPY' named entity 'PREPARED' named entity 'FORMATION' named entity 'GLYCAN ARRAY' named entity 'SLIDES' named entity 'ACID' named entity 'REPORT' named entity 'CONFORMATION' named entity 'BASED' named entity 'VAPOR' named entity 'GLYCAN' named entity 'GLASS' named entity 'N-HYDROXYSUCCINIMIDE' named entity 'ALUMINUM' named entity 'SUGAR' named entity 'SLIDE' named entity 'MODEL' named entity 'SLIDES' named entity 'PROBE' named entity 'FABRICATION' named entity 'ATOMIC FORCE MICROSCOPY' named entity 'PHOSPHONATE' named entity 'POLYMERIZATION' named entity 'DEPOSITION' named entity 'CONCANAVALIN A' named entity 'SURFACE' named entity 'DENSITY' named entity '300' named entity 'OPTIMIZATION' named entity 'MICROARRAYS' named entity 'SYSTEM' named entity 'SPATIAL' named entity 'GENEPIX' named entity 'TAGGED'

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