About: A missense polymorphism in porcine interferon-γ cDNA affects antiviral activity of the protein variant

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About: A missense polymorphism in porcine interferon-γ cDNA affects antiviral activity of the protein variant Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A missense polymorphism in porcine interferon-γ cDNA affects antiviral activity of the protein variant
Creator	Fan, Yi-Hsin Chow, Kuan-Chih Chi, Lang-Ming Chiou, Shiow-Her Huang, Chienjin Huang, San-Yuan
Source	Elsevier; Medline; PMC
abstract	Abstract We determined the interferon-γ (IFN-γ) cDNA sequence from three porcine breeds, Duroc, Landrance/Duroc hybrid, and Landrance breeds. Five single nucleotide polymorphisms (SNPs) of porcine IFN-γ (PoIFN-γ) were identified, respectively, at positions 269 (A/G), 376 (C/T), 426 (T/C), and 465 (T/C) of the coding sequence in Landrance/Duroc hybrid, and at position 251 (A/G) in Landrance breed. Among them, A269G and A251G polymorphisms resulted in Q67R and K61R replacements in the mature protein. PoIFN-γ cDNAs of Duroc breed (PoIFN-γ-W) and Landrance/Duroc hybrid (PoIFN-γ-M), which, respectively, encoded Q67 and R67, were introduced into a prokaryotic expression vector pET32 to express recombinant PoIFN-γ-W (rPoIFN-γ-W) and rPoIFN-γ-M protein variants in Escherichia coli. The identity of both protein variants was further confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We then compared bioactivities of these two recombinant proteins. Although both recombinant protein variants exhibited comparable activities in antiproliferation of PK-15 cells and in nitric oxide (NO) induction of porcine peripheral monocytes, antiviral activity of rPoIFN-γ-W protein was significantly higher (P <0.001) than that of rPoIFN-γ-M protein in a plaque inhibition assay using pseudorabies virus (PRV). IC50 values of rPoIFN-γ-W and rPoIFN-γ-M protein in anti-PRV assay were determined as 5.3±1.3 and 9.3±4.3nM, respectively. In conclusion, we have identified five novel SNPs in PoIFN-γ cDNA, including two missense polymorphisms that result in Q67R and K61R replacements. Our results further demonstrate that Q67R can markedly reduce antiviral activity of the PoIFN-γ protein. This is the first report that shows the functional SNP in the coding region of IFN-γ. In the future, it is imperative to determine whether Q67R replacement in IFN-γ may have disease association.
has issue date	2007-07-31(xsd:dateTime)
bibo:doi	10.1016/j.molimm.2007.02.029
bibo:pmid	17416419
has license	els-covid
sha1sum (hex)	def2c6aa475f309fa8a2c450c71410fd316c5cdf
schema:url	https://doi.org/10.1016/j.molimm.2007.02.029
resource representing a document's title	A missense polymorphism in porcine interferon-γ cDNA affects antiviral activity of the protein variant
has PubMed Central identifier	PMC7112673
has PubMed identifier	17416419
schema:publication	Molecular Immunology
resource representing a document's body	covid:def2c6aa475f309fa8a2c450c71410fd316c5cdf#body_text
is schema:about of	named entity 'MISSENSE' named entity 'interferon' named entity 'peripheral' named entity 'mature' named entity 'identity' named entity 'Duroc' named entity 'cDNA' named entity 'determined' named entity 'activities' named entity 'IC50' named entity 'CODING SEQUENCE' named entity '90%' named entity 'COMPARED' named entity 'M PROTEIN' named entity 'SINGLE NUCLEOTIDE POLYMORPHISMS' named entity 'ESCHERICHIA COLI' named entity 'DUROC' covid:arg/def2c6aa475f309fa8a2c450c71410fd316c5cdf named entity 'ENCODED' named entity 'IDENTIFIED' named entity 'CDNA SEQUENCE' named entity '1.3' named entity 'LASER DESORPTION' named entity 'CELLS' named entity 'RECOMBINANT' named entity 'DETERMINED' named entity 'VALUES' named entity 'NITRIC OXIDE' named entity 'ACTIVITIES' named entity 'Q67' named entity 'CONFIRMED BY' named entity '28A' named entity 'PROKARYOTIC EXPRESSION' named entity 'POLYMORPHISMS' named entity 'IFN' named entity 'POSITION' named entity 'BREEDS' named entity 'IDENTITY' named entity 'HYBRID' named entity 'INTERFERON' named entity 'PLAQUE' named entity '28P' named entity 'TIME-OF-FLIGHT MASS SPECTROMETRY' named entity 'PERIPHERAL' named entity 'HIGHER' named entity 'THESE' named entity 'USING' named entity 'EXPRESSION VECTOR' named entity 'TO EXPRESS' named entity '376' named entity 'ASSISTED' named entity 'PORCINE' named entity 'INTERFERON' named entity 'PORCINE' named entity 'PROTEIN VARIANT' named entity 'POLYMORPHISM' named entity 'CDNA' named entity '4.3' named entity 'PROTEIN ' named entity 'INTRODUCED INTO' named entity 'TOF MS' named entity 'PRV' named entity '5.3' named entity '426' named entity 'INDUCTION' named entity 'RECOMBINANT PROTEIN' named entity 'POSITIONS' named entity 'MONOCYTES' named entity 'ANTIVIRAL ACTIVITY' named entity 'ASSAY' named entity 'PROTEIN VARIANTS' named entity 'REPLACEMENTS' named entity 'RECOMBINANT PROTEINS' named entity 'BREED' named entity 'INHIBITION' named entity '0.001'

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