About: Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers

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About: Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers
Creator	Gismondo, Maria Mancon, Alessandro Mileto, Davide Comandatore, Francesco Gigantiello, Anna Longobardi, Concetta Maresca, Mafalda Moja, Lorenzo Pagani, Cristina Polesello, Stefano Rimoldi, Sara Romeri, Francesca Salerno, Franco Stefani, Fabrizio
Source	MedRxiv
abstract	Wastewater-based epidemiology has been proposed to monitor the diffusion and trend of SARS-CoV-2 pandemic. In the present study, raw and treated samples from three wastewater treatment plants, and two river samples characterized the Milano Metropolitan Area, Italy, were surveyed for SARS-CoV-2 RNA positivity to real time PCR and infectiveness. Moreover, whole genome sequencing and phylogenetic analysis of isolated strains was performed. Raw wastewater samples resulted positive to PCR amplification, while treated water samples were always negative (four and two samples, respectively, sampled in two dates). Moreover, the rate of positivity in raw wastewater samples decreased after eight days, in congruence with the epidemiological trend estimated for the interested provinces. Virus infectiveness was always not significant, indicating the effectiveness of wastewater treatments, or the natural decay of viral vitality, which implied the absence of significant risk of infection from wastewaters. Samples from receiving rivers (two sites, sampled in the same dates as wastewaters) showed in some cases a positivity to PCR amplification, probably due to non-treated discharges, or the combined sewage overflows. Nevertheless, also for rivers vitality was negligible, indicating the absence of sanitary risks. Phylogenetic analysis of genome indicated that the isolated virus belongs to the most spread strain present in Europe and similar to another strain found in Lombardy.
has issue date	2020-05-05(xsd:dateTime)
bibo:doi	10.1101/2020.05.01.20086009
has license	medrxiv
sha1sum (hex)	dde8f534b43dd44041a4820eee0849e70842ae28
schema:url	https://doi.org/10.1101/2020.05.01.20086009
resource representing a document's title	Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers
resource representing a document's body	covid:dde8f534b43dd44041a4820eee0849e70842ae28#body_text
is schema:about of	named entity 'positivity' named entity 'SARS-CoV-2' named entity 'wastewaters' named entity 'PHYLOGENETIC ANALYSIS' named entity 'ITALY' named entity 'performed' named entity 'haplotype' named entity 'multiplex PCR' named entity 'SARS-CoV-2' named entity 'viral RNA' named entity 'common origin' named entity 'gene' named entity 'Promega' named entity 'Milan' named entity 'SARS-CoV-2' named entity 'gene' named entity 'epidemic' named entity 'infection' named entity 'genome' named entity 'China' named entity 'sewage' named entity 'epidemic' named entity 'SARS-CoV-2' named entity 'CC-BY-NC-ND 4.0' named entity 'Enveloped virus' named entity 'RNA' named entity '2019-nCoV' named entity 'medRxiv' named entity 'Italian' named entity 'March 11' named entity 'Randazzo' named entity 'Maximum Likelihood' named entity 'PCR' named entity 'RNA' named entity 'cell line' named entity 'nucleocapsid' named entity 'metazoan' named entity 'significant source' named entity 'virus' named entity 'study' named entity 'SARS-CoV-2 pandemic' named entity 'real time PCR' named entity 'Italy' named entity 'Medema' named entity 'Elbe' named entity 'real time PCR' named entity 'viral load' named entity 'medRxiv' named entity 'SARS-CoV-2' named entity 'epidemy' named entity 'Italy' named entity 'SARS-CoV-2' named entity 'RNA' named entity 'SARS-like coronaviruses' named entity 'cell culture' named entity 'genome' named entity 'SNPs' named entity 'infection' named entity 'log10' named entity 'RNA' named entity 'virus' named entity 'phylogenetic analysis' named entity 'kidney' named entity 'DMEM' named entity 'SARS-CoV-2' named entity 'Gona' named entity 'RNA' named entity 'CO2' named entity 'peer review' named entity 'SARS-CoV' named entity 'ORF1' named entity 'RNA'

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