About: Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)
Creator	Fishbein, L O'hern, T~
Source	PMC
abstract	The histo-inhibitory factor (HIF) derived from homogenates of liver or kidney from hamsters infected withHistoplasma capsulatum has been fractionated by column chromatography. It shows maximum absorption at 280 mµ, has a molecular weight of 142,000 and can be eluted from DEAE-cellulose or DEAE-Sephadex A—50 with 0.02 M phosphate — 4 M sodium chloride (1 : 1) HIF can be eluted fromHistoplasma yeast cells at pH 10.0, thus a greater number of positive cultures from chronic histoplasmosis could be expected to result from pretreatment of clinical specimens in glycine buffer pH 10.0 prior to culture.
has issue date	1968-01-01(xsd:dateTime)
bibo:doi	10.1007/bf02057164
bibo:pmid	5748624
has license	no-cc
sha1sum (hex)	db07dc8be7f36e3f94796f2f0723bdea1493c9eb
schema:url	https://doi.org/10.1007/bf02057164
resource representing a document's title	Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)
has PubMed Central identifier	PMC7101975
has PubMed identifier	5748624
schema:publication	Mycopathol Mycol Appl
resource representing a document's body	covid:db07dc8be7f36e3f94796f2f0723bdea1493c9eb#body_text
is schema:about of	named entity 'saline' named entity 'homogenizer' named entity 'anion exchanger' named entity 'infection' named entity 'serum albumin' named entity 'ELUTION' named entity 'FACTOR' named entity 'phosphate' named entity 'sodium hydroxide' named entity 'saline' named entity 'Histoplasma' named entity 'pH 10' named entity 'yeast cells' named entity 'fractionation' named entity 'DEAE' named entity 'kidney' named entity 'molecular weight' named entity 'negative control' named entity 'pH 10' named entity 'sodium chloride' named entity 'supernatant' named entity 'pH 10' named entity 'H. capsulatum' named entity 'molecular weights' named entity 'pH 10' named entity 'Bacillus subtilis' named entity 'ovalbumin' named entity 'yeast cells' named entity 'glycine' named entity 'Blastomyces dermatitidis' named entity 'Biochemicals' named entity 'sieve' named entity 'bovine' named entity 'centrifuged' named entity 'bacteria' named entity 'antibody' named entity 'void volume' named entity 'eluted' named entity 'molecular weight' named entity 'Sephadex' named entity 'Elution' named entity 'NaOH' named entity 'saline' named entity 'protein' named entity 'glycine' named entity 'Escherichia coli' named entity 'liver' named entity 'molecular weight' named entity 'Sephadex' named entity 'Sephadex' named entity 'spectrophotometric' named entity '1 cm' named entity 'yeast cells' named entity 'hemoglobin' named entity 'positive control' named entity 'Histoplasma' named entity 'ovalbumin' named entity 'saline' named entity 'yeast cells' named entity 'ormans' named entity 'protein' named entity 'inoculation' named entity 'kidney' named entity 'hemoglobin' named entity 'ovalbumin' named entity 'DEAE-cellulose' named entity 'kidney' named entity 'elution' named entity 'supernatants' named entity 'molecular weight' named entity 'filtration' named entity 'centrifuged' named entity 'yeast cells' named entity 'Sephadex' named entity 'molecular weights' named entity 'liver' named entity 'infection' named entity 'saline' named entity 'Void volume' named entity 'supernatants'

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