About: Assessment of periradicular microbiota by DNA‐DNA hybridization

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About: Assessment of periradicular microbiota by DNA‐DNA hybridization Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Assessment of periradicular microbiota by DNA‐DNA hybridization
Creator	Lind, P Assessment, Olsen Er, Eribe Eribe, E Olsen, I Po, Lind Pt, Sunde Sunde, P Tronstad, L
Source	PMC
abstract	Abstract – In the present study the “checkerboard” DNA‐DNA hybridization technique was used to identify bacteria in periapical endodontic lesions of asymptomatic teeth. Thirty‐four patients with root‐filled teeth and apical periodontitis were divided into two groups, each containing 17 patients. In Group 1, a marginal incision was performed during surgery to expose the lesion, and in Group 2, a submarginal incision was applied. The gingiva and mucosa were swabbed with an 0.2% chlorhexidine gluconate solution prior to surgery. Bacterial DNA was identified in all samples from the two groups using 40 different whole genomic probes. The mean number (±SD) of species detected was 33.7±3.3 in Group 1 and 21.3±6.3 in Group 2 (P<0.001). The majority of the probe‐detected bacteria were present in more lesions from Group1 than from Group 2. The differences were most notable for Campylobacter gracilis, Porphyromonas endodontalis, Propionibacterium acnes, Capnocytophaga gingivalis, Fusobacterium nucleatum ssp. nucleatum, Fusobacterium nucleatum ssp. polymorphum, Prevotella intermedia, Treponema denticola, Streptococcus constellatus and Actinomyces naeslundii I. Bacterial species such as Actinobacillus actinomycetemcomitans and Bacteroides forsythus were detected in more than 60% of the lesions from both groups. Also, P. endodontalis was abundant in periapical tissue. The data supported the idea that following a marginal incision, bacteria from the periodontal pocket might reach the underlying tissues by surgeon‐released bacteremia. The study provided solid evidence that bacteria invade the periapical tissue of asymptomatic teeth with apical periodontitis. The detection of much more bacteria with the “checkerboard” DNA‐DNA hybridization method than has previously been recovered by anaerobic culture indicated that the endodontic (and periodontal) microfloras should be redefined using molecular methods.
has issue date	2001-12-24(xsd:dateTime)
bibo:doi	10.1034/j.1600-9657.2000.016005191.x
bibo:pmid	11202881
has license	no-cc
sha1sum (hex)	d4fd980278883801d7d4cc4f13504de55b612905
schema:url	https://doi.org/10.1034/j.1600-9657.2000.016005191.x
resource representing a document's title	Assessment of periradicular microbiota by DNA‐DNA hybridization
has PubMed Central identifier	PMC7194203
has PubMed identifier	11202881
schema:publication	Dent Traumatol
resource representing a document's body	covid:d4fd980278883801d7d4cc4f13504de55b612905#body_text
is schema:about of	named entity 'lesions' named entity 'abundant' named entity 'periodontal pocket' named entity 'periodontitis' named entity 'Key' named entity 'apical' named entity 'anaerobic' named entity 'checkerboard' named entity 'tissue' named entity 'gingiva' named entity 'Group 1' named entity 'groups' named entity 'tissues' named entity 'reach' named entity 'periapical' named entity 'Capnocytophaga' named entity 'The majority' named entity 'Actinobacillus' named entity 'teeth' named entity 'Pia' named entity 'teeth' named entity 'prior' named entity 'Fusobacterium nucleatum' named entity 'Fusobacterium nucleatum' named entity 'apical periodontitis' named entity 'DNA-DNA hybridization' named entity 'periapical' named entity 'Treponema denticola' named entity 'endodontic' named entity 'infection' named entity 'periapical' named entity 'anaerobic' named entity 'Capnocytophaga' named entity 'endodontic' named entity 'bacteria' named entity 'DNA-DNA hybridization' named entity 'periradicular' named entity 'infection' named entity 'bacterial flora' named entity 'apical periodontitis' named entity 'statistically significant' named entity 'bacteria' named entity 'T. denticola' named entity 'endodontic' named entity 'lesion' named entity 'periapical lesions' named entity 'endodontic' named entity 'bacteria' named entity 'bacteria' named entity 'spirochetes' named entity 'casein' named entity 'Campylobacter' named entity 'spp' named entity 'Fusobacterium nucleatum' named entity 'microorganisms' named entity 'periosteal' named entity 'endodontic' named entity 'periapical' named entity 'root canal' named entity 'Treponema denticola' named entity 'xylocaine' named entity 'bacteria' named entity 'DNA-DNA hybridization' named entity 'spp' named entity 'casein' named entity 'periodontium' named entity 'P-value' named entity 'anaerobic' named entity 'detection limit' named entity 'ammonium acetate' named entity 'periodontitis' named entity 'bacteria' named entity '1, 2'

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