About: Alternative Polyadenylation of Human Bocavirus at Its 3′ End Is Regulated by Multiple Elements and Affects Capsid Expression

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About: Alternative Polyadenylation of Human Bocavirus at Its 3′ End Is Regulated by Multiple Elements and Affects Capsid Expression Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Alternative Polyadenylation of Human Bocavirus at Its 3′ End Is Regulated by Multiple Elements and Affects Capsid Expression
Creator	Wang, Hanzhong Chen, Zhen Guan, Wuxiang Hao, Sujuan Xu, Huanzhou Zhang, Junmei
Source	PMC
abstract	Alternative processing of human bocavirus (HBoV) P5 promoter-transcribed RNA is critical for generating the structural and nonstructural protein-encoding mRNA transcripts. The regulatory mechanism by which HBoV RNA transcripts are polyadenylated at proximal [(pA)p] or distal [(pA)d] polyadenylation sites is still unclear. We constructed a recombinant HBoV infectious clone to study the alternative polyadenylation regulation of HBoV. Surprisingly, in addition to the reported distal polyadenylation site, (pA)d, a novel distal polyadenylation site, (pA)d2, which is located in the right-end hairpin (REH), was identified during infectious clone transfection or recombinant virus infection. (pA)d2 does not contain typical hexanucleotide polyadenylation signal, upstream elements (USE), or downstream elements (DSE) according to sequence analysis. Further study showed that HBoV nonstructural protein NS1, REH, and cis elements of (pA)d were necessary and sufficient for efficient polyadenylation at (pA)d2. The distance and sequences between (pA)d and (pA)d2 also played a key role in the regulation of polyadenylation at (pA)d2. Finally, we demonstrated that efficient polyadenylation at (pA)d2 resulted in increased HBoV capsid mRNA transcripts and protein translation. Thus, our study revealed that all the bocaviruses have distal poly(A) signals on the right-end palindromic terminus, and alternative polyadenylation at the HBoV 3′ end regulates its capsid expression. IMPORTANCE The distal polyadenylation site, (pA)d, of HBoV is located about 400 nucleotides (nt) from the right-end palindromic terminus, which is different from those of bovine parvovirus (BPV) and canine minute virus (MVC) in the same genus whose distal polyadenylation is located in the right-end stem-loop structure. A novel polyadenylation site, (pA)d2, was identified in the right-end hairpin of HBoV during infectious clone transfection or recombinant virus infection. Sequence analysis showed that (pA)d2 does not contain typical polyadenylation signals, and the last 42 nt form a stem-loop structure which is almost identical to that of MVC. Further study showed that NS1, REH, and cis elements of (pA)d are required for efficient polyadenylation at (pA)d2. Polyadenylation at (pA)d2 enhances capsid expression. Our study demonstrates alternative polyadenylation at the 3′ end of HBoV and suggests an additional mechanism by which capsid expression is regulated.
has issue date	2017-01-18(xsd:dateTime)
bibo:doi	10.1128/jvi.02026-16
bibo:pmid	27881651
has license	cc-by
sha1sum (hex)	d474315fbb8b2c284039f0caec40aaa334db068b
schema:url	https://doi.org/10.1128/jvi.02026-16
resource representing a document's title	Alternative Polyadenylation of Human Bocavirus at Its 3′ End Is Regulated by Multiple Elements and Affects Capsid Expression
has PubMed Central identifier	PMC5244319
has PubMed identifier	27881651
schema:publication	J Virol
resource representing a document's body	covid:d474315fbb8b2c284039f0caec40aaa334db068b#body_text
is schema:about of	named entity 'ITS' named entity 'polyadenylation' named entity 'USE' named entity 'nonstructural protein' named entity 'polyadenylation' named entity 'efficient' named entity 'efficient' named entity 'CAPSID' named entity 'ACCORDING' named entity 'PROTEIN TRANSLATION' named entity '400' named entity 'ALTERNATIVE POLYADENYLATION' named entity 'TRANSCRIPTS' named entity 'CONSTRUCTED' named entity 'UNCLEAR' named entity 'ITS' named entity 'REVEALED' named entity 'GENUS' named entity 'ADDITIONAL' named entity 'RECOMBINANT VIRUS' named entity 'MVC' named entity 'REQUIRED' named entity 'NOVEL' named entity 'SUFFICIENT' named entity 'CRITICAL' named entity 'REGULATED' named entity 'OUR' named entity 'DIFFERENT' named entity 'POLYADENYLATION SITE' named entity 'ALTERNATIVE' named entity 'GENERATING' named entity 'EFFICIENT' named entity 'PROXIMAL' named entity 'BOVINE PARVOVIRUS' named entity 'MECHANISM' named entity '29P' named entity 'IDENTICAL' named entity 'STRUCTURAL' named entity 'STUDY' named entity 'DISTAL' named entity 'IDENTIFIED' named entity 'INCREASED' named entity 'RIGHT' named entity 'CONTAIN' named entity 'ALTERNATIVE POLYADENYLATION' named entity 'TRANSCRIBED' named entity 'STEM-LOOP STRUCTURE' named entity 'HAVE' named entity 'HUMAN BOCAVIRUS' named entity 'DOWNSTREAM' named entity 'PROCESSING' named entity 'CIS' named entity 'POLYADENYLATED' named entity 'ABOUT' named entity 'SEQUENCE ANALYSIS' named entity 'LOCATED IN' named entity 'SITES' named entity 'PLAYED' named entity 'RNA' named entity 'UPSTREAM' named entity 'MRNA' named entity 'REGULATED' named entity 'NUCLEOTIDES' named entity 'END' named entity 'ALMOST' named entity 'PROMOTER' named entity 'END' named entity 'MULTIPLE' named entity 'HUMAN BOCAVIRUS' named entity 'ELEMENTS' named entity 'RECOMBINANT' named entity 'POLY' named entity 'TYPICAL' named entity 'SIGNAL' named entity 'TERMINUS' named entity 'ADDITION'

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