About: Defective RNAs of clover yellow mosaic virus encode nonstructural/coat protein fusion products

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Defective RNAs of clover yellow mosaic virus encode nonstructural/coat protein fusion products
Creator	White, K Bancroft, J Mackie, George
topic	covid:d27604acfc3b51984800e673093f11ce96512801#this
Source	Elsevier; Medline; PMC
abstract	Abstract A small group of 1.2-kb RNAs present on polyribosoes from clover yellow mosaic virus (CYMV)-infected tissue contains sequences from the genomic RNA (gRNA) of CYMV and is encapsidated by CYMV coat protein. Some features of these RNAs suggest that they are similar to defective interfering (DI) RNAs, and would be the first to be reported for the potexvirus group. The prototype 1.2-kb RNA is 1172 nucleotides in length excluding a probable poly(A) tail and is composed of two noncontiguous regions corresponding to 757 nucleotides of the 5′ and 415 nucleotides of the 3′ terminal of CYMV's gRNA. The sequence of the prototype 1.2-kb RNA reveals that the two terminal gRNA regions present in this RNA encode a single open reading frame (ORF) joining the N-terminus of the 191-kDa nonstructural product and the C-terminus of the coat protein to form a 35-kDa 191-kDa/coat protein fusion product. The coding properties of this prototype RNA have been confirmed by translation in vitro of native and synthetic transcripts of the 1.2-kb RNAs, both of which direct the synthesis of the anticipated 35-kDa product which reacts with anti-CYMV antiserum. Three additional 1.2-kb RNA species, each of which contains a unique junction site, have been characterized. In all cases, a fusion ORF encoding a 191-kDa/coat protein fusion product is encoded on the RNA. The presence of a fusion ORF in all members of the 1.2-kb RNA species analyzed suggests that maintenance of this ORF may be important for the survival of this class of RNA within the plant. This coding strategy represents a novel property of plant virus defective RNAs.
has issue date	1991-08-31(xsd:dateTime)
bibo:doi	10.1016/0042-6822(91)90977-j
bibo:pmid	1830181
has license	els-covid
sha1sum (hex)	d27604acfc3b51984800e673093f11ce96512801
schema:url	https://doi.org/10.1016/0042-6822%2891%2990977-j
resource representing a document's title	Defective RNAs of clover yellow mosaic virus encode nonstructural/coat protein fusion products
has PubMed Central identifier	PMC7130666
has PubMed identifier	1830181
schema:publication	Virology
resource representing a document's body	covid:d27604acfc3b51984800e673093f11ce96512801#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/d27604acfc3b51984800e673093f11ce96512801
is schema:about of	named entity 'DEFECTIVE' named entity 'CLOVER YELLOW MOSAIC VIRUS' named entity 'RNAS' named entity 'PRODUCTS' named entity 'PROTEIN FUSION' named entity 'ENCODE' named entity 'Nonstructural' named entity 'Clover Yellow Mosaic Virus' named entity 'RNAs' named entity 'TBSV' named entity '1.2' named entity 'RNA' named entity 'RNA' named entity 'Promega' named entity '5.8' named entity 'cDNA' named entity '1.2' named entity 'RNAs' named entity 'gRNA' named entity 'tombusvirus' named entity 'CYMV' named entity 'CYMV' named entity 'amino acid sequence' named entity '1.2' named entity 'Pharmacia' named entity '1.2' named entity 'Taq DNA polymerase' named entity 'CYMV' named entity 'gRNA' named entity 'Promega' named entity 'deoxythymidylate' named entity 'virus' named entity 'transcription' named entity 'CYMV' named entity 'reticulocyte' named entity 'RNAs' named entity 'nucleotide' named entity 'CYMV' named entity 'gRNA' named entity 'RNA' named entity 'virions' named entity 'RNA' named entity 'RNA' named entity 'Clover yellow mosaic virus' named entity 'immunological' named entity 'plasmid DNA' named entity 'poly(A) tail' named entity 'viral infections' named entity 'Clover yellow mosaic virus' named entity 'CYMV' named entity 'satellite RNAs' named entity 'Clover yellow mosaic virus' named entity 'symptom' named entity 'kDa' named entity 'Clover yellow mosaic virus' named entity 'diluent' named entity 'coat protein' named entity 'Clover yellow mosaic virus' named entity 'denatured' named entity 'protein' named entity '3' terminus' named entity 'Clover yellow mosaic virus' named entity 'RNAs' named entity 'RNAs' named entity 'RNA' named entity 'nucleoprotein' named entity 'centrifugation' named entity 'Cloned' named entity 'tomato bushy stunt virus' named entity 'gRNA' named entity 'Promega' named entity 'RNAs' named entity 'single-base substitutions' named entity '5' end' named entity '3' ends' named entity 'coat protein' named entity 'Clover yellow mosaic virus'

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