About: Comparison of Different Kits for SARS-CoV-2 RNA Extraction Marketed in Brazil

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About: Comparison of Different Kits for SARS-CoV-2 RNA Extraction Marketed in Brazil Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of Different Kits for SARS-CoV-2 RNA Extraction Marketed in Brazil
Creator	Hansen, Alana Spilki, Fernando Demoliner, Meriane Eisen, Antunes Fleck, Juliane Heldt, Henrique Hermann, Bruna Karolina, Ana Mallmann, Larissa Rodrigues De Almeida, Paula Schallenberger, Karoline Schons Gularte, Juliana
Source	BioRxiv
abstract	December 2019 marked the begining of the greatest pandemic since Spanish Flu, the disease named Covid-19 that cause severe pneumonia. Until May 19, 2020 more than 4 million and 700 thousand cases were oficially notified with about 316 thousand deaths. Etiological agent of the disease was identified as being a new coronavirus, Severe acute respiratory syndrome-related coronavirus (SARS-CoV-2). In this study we compared four different manual methods for RNA isolation and purification for detection of SARS-CoV-2 through qRT-PCR, as well as the extraction quality itself through detection of RNAse P. Magnetic beads-based (MagMax™) and silica column-based (Biopur®) methods presented the better performances. Concerning to the mean delay in CT values when compared to MagMax™, TRIzol™, Biopur® and EasyExtract presented 0,39, 0,95 and 5,23 respectively. Agreement between positive and negative results of different methods when compared with the one with better performance MagMax™ was 94,44% for silica column-based method (Biopur®), 88,89% for phenol-chroloform-based method (TRIzol™) and 77,78% for EasyExtract. We aimed to evaluate how reliable each method is for diagnostic purposes and to propose alternatives when usual methods are not available. In this regard, magnectic beads and silica column-based methods are convenient and reliable choices and phenol-chloroform-based method could also be chosen as an alternative.
has issue date	2020-05-29(xsd:dateTime)
bibo:doi	10.1101/2020.05.29.122358
has license	biorxiv
sha1sum (hex)	d1b1d88020ce55a1da0ac03a5803b92ba8dcc3dc
schema:url	https://doi.org/10.1101/2020.05.29.122358
resource representing a document's title	Comparison of Different Kits for SARS-CoV-2 RNA Extraction Marketed in Brazil
schema:publication	bioRxiv
resource representing a document's body	covid:d1b1d88020ce55a1da0ac03a5803b92ba8dcc3dc#body_text
is schema:about of	named entity 'severe' named entity 'SARS-COV-2' named entity 'DIFFERENT' named entity 'disease' named entity 'KITS' named entity 'severe pneumonia' named entity 'Covid' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'Wuhan' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'column-based methods' named entity 'SARS-CoV-2' named entity 'silica column' named entity 'Europe' named entity 'single stranded RNA' named entity 'nucleic acids' named entity 'chloroform' named entity 'qRT-PCR' named entity 'phenol' named entity 'TRIzol' named entity 'bacteria' named entity 'human gut' named entity 'SARS' named entity 'vaccines' named entity 'MagMax' named entity 'lipid' named entity 'RNA' named entity 'phenol' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'phenol' named entity 'respiratory disease' named entity 'gene' named entity 'cell culture' named entity 'qRT-PCR' named entity 'vomiting' named entity 'Phenol' named entity 'SARS-CoV-2' named entity 'Nucleic acids' named entity 'MagMax' named entity 'China' named entity 'TRIzol' named entity 'minimum essential medium' named entity 'phenol' named entity 'atypical pneumonia' named entity 'RNA' named entity 'lysis' named entity 'nucleic acids' named entity 'MERS-CoV' named entity 'MEM' named entity 'Brazil' named entity 'MagMax' named entity 'chloroform' named entity 'coronavirus' named entity 'OC43' named entity 'MagMax' named entity 'NL63' named entity 'qRT-PCR' named entity 'MagMax' named entity 'viral titers' named entity 'Covid' named entity 'Nucleic Acid' named entity 'viruses' named entity 'TRIzol' named entity 'Covid' named entity 'pangolins' named entity 'microbial' named entity 'sore throat' named entity 'Covid' named entity 'qRT-PCR' named entity 'PCR amplification'

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