About: Development and application of a simple recombinase polymerase amplification assay for rapid point-of-care detection of feline herpesvirus type 1

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About: Development and application of a simple recombinase polymerase amplification assay for rapid point-of-care detection of feline herpesvirus type 1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and application of a simple recombinase polymerase amplification assay for rapid point-of-care detection of feline herpesvirus type 1
Creator	Chen, Ze-Liang Han, Xiao-Hu Liu, Bao-Shan Liu, Meng-Zhi Long-Quan Yao, · Zhang, Wen-Kui
source	Medline; PMC
abstract	Feline herpesvirus type 1 (FHV-1) is a highly contagious pathogen of domestic cats and other members of the family Felidae. Point-of-care diagnosis of persistent infection in cats is essential for control of its spread. A recombinase polymerase amplification (RPA) assay (RPA-LFD-FHV) combined with a lateral flow dipstrip (LFD) was developed that uses human body heat for incubation. Sensitivity was evaluated by testing a serial dilution of a control plasmid, and specificity was evaluated by testing related viruses. Swab samples from cats with suspected infection were tested by RPA-LFD-FHV, and the results were compared to those obtained by PCR to evaluate its clinical performance. The RPA-FLD-FHV assay was carried out successfully within 20 min, using body heat for incubation. The RPA-FLD-FHV had a detection limit of 10(3) copies of the FHV-1 gD gene, which was lower than that of PCR, which was 10(4) copies. The assay could detect templates of FHV-1 but not those of other feline and canine viruses. Viruses in boiled samples could be efficiently detected by the RPA-FLD-FHV. Thirty-one out of the 80 samples were positive by the RPA-FLD-FHV assay, whereas only 27 were positive by PCR. DNA sequencing confirmed that the four samples that were positive by RPA-FLD-FHV but negative by PCR were indeed positive. These results indicate that RPA-FLD-FHV is applicable for clinical use. The RPA-FLD-FHV assay is a simple, rapid, and reliable method for point-of-care diagnosis of FHV-1 infection.
has issue date	2018-10-09(xsd:dateTime)
bibo:doi	10.1007/s00705-018-4064-7
bibo:pmid	30302584
has license	no-cc
sha1sum (hex)	cf3747f8dc2c729cc6d707531dd474ea2ebc222a
schema:url	https://doi.org/10.1007/s00705-018-4064-7
resource representing a document's title	Development and application of a simple recombinase polymerase amplification assay for rapid point-of-care detection of feline herpesvirus type 1
has PubMed Central identifier	PMC7086775
has PubMed identifier	30302584
schema:publication	Arch Virol
resource representing a document's body	covid:cf3747f8dc2c729cc6d707531dd474ea2ebc222a#body_text
is schema:about of	named entity 'ASSAY' named entity 'samples' named entity 'persistent' named entity 'DNA sequencing' named entity 'RPA' named entity 'canine' named entity 'application' named entity 'SWAB' named entity 'BODY' named entity 'COPIES' named entity 'DILUTION' named entity 'SERIAL' named entity 'CATS' named entity 'HEAT' named entity 'LOWER' named entity 'SIMPLE' named entity 'HUMAN BODY' named entity 'CONFIRMED' named entity 'RPA' named entity 'SUSPECTED' named entity 'DIAGNOSIS' named entity 'ITS' named entity 'LFD' named entity 'VIRUSES' named entity 'SIMPLE' named entity 'DETECTION' named entity 'DEVELOPMENT' named entity 'FHV' named entity 'OBTAINED BY' named entity 'DETECTED' named entity 'FAMILY FELIDAE' named entity 'INFECTION' named entity 'CARRIED' named entity 'POINT-OF-CARE' named entity 'FELINE HERPESVIRUS TYPE 1' named entity 'RAPID' named entity 'AMPLIFICATION' named entity 'RECOMBINASE' named entity 'APPLICATION' named entity 'POLYMERASE' named entity 'SPECIFICITY' named entity 'EVALUATE' named entity 'USING' named entity 'SPREAD' named entity 'CONTROL' named entity 'AMPLIFICATION' named entity 'GENE' named entity 'POINT-OF-CARE' named entity 'EVALUATED' named entity 'INCUBATION' named entity 'METHOD' named entity 'FLD' named entity 'RESULTS' named entity 'SENSITIVITY' named entity 'POSITIVE' named entity 'IS A' named entity 'FLOW' named entity 'USE' named entity 'DNA SEQUENCING' named entity 'SAMPLES' named entity 'THIRTY-ONE' named entity 'DETECTION LIMIT' named entity 'BOILED' named entity 'NEGATIVE' named entity 'PCR' named entity 'PLASMID' named entity 'TEMPLATES' named entity 'LATERAL' named entity 'PATHOGEN' named entity 'FELINE' named entity 'CANINE' named entity 'PERFORMANCE' named entity 'ESSENTIAL' named entity 'RAPID' named entity 'ASSAY' named entity 'PERSISTENT INFECTION'

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