About: A Small Region of Porcine Hemagglutinating Encephalomyelitis Virus Spike Protein Interacts with the Neural Cell Adhesion Molecule

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A Small Region of Porcine Hemagglutinating Encephalomyelitis Virus Spike Protein Interacts with the Neural Cell Adhesion Molecule Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Small Region of Porcine Hemagglutinating Encephalomyelitis Virus Spike Protein Interacts with the Neural Cell Adhesion Molecule
Creator	Gao, Feng Gao, Wei He, Wenqi Lan, Yungang Lu, Huijun Zhao, Kui Ding, Ning Tang, Bo Dong, Bo Jin, Zhao Zhao, Jiakuan Liu, Wenfeng
Source	Medline; PMC
abstract	OBJECTIVE: The spike (S) protein of porcine hemagglutinating encephalomyelitis virus (PHEV) may mediate infection by binding to a cellular neural cell adhesion molecule (NCAM). This study aimed to identify the crucial domain of the S1 subunit of the S protein that interacts with NCAM. METHODS: Three truncated segments (S(1-291), S(277-794) and S(548-868)) of the S gene of PHEV and the NCAM gene were cloned individually into the Escherichia coli expression vectors and yeast two-hybrid expression vectors. The interaction between S(1-291), S(277-794), S(548-868) and NCAM were detected by a GST pull-down experiment and yeast two-hybrid assay. RESULTS: Three fusion proteins (S(1-291), S(277-794) and S(548-868)) were screened for their interactions with NCAM by protein-protein interaction assays. The results of these assays clarified that S(277-794) interacted with NCAM, while S(1-291) and S(548-868) did not. CONCLUSIONS: A small fragment (258-amino-acid fragment, residues 291-548) on the PHEV S protein was posited to be the minimum number of amino acids necessary to interact with NCAM. This fragment may be the receptor-binding domain that mediates PHEV binding to NCAM.
has issue date	2015-04-25(xsd:dateTime)
bibo:doi	10.1159/000381060
bibo:pmid	25925196
has license	no-cc
sha1sum (hex)	ceaf1ce77440c43549de7a989c28376495b57e61
schema:url	https://doi.org/10.1159/000381060
resource representing a document's title	A Small Region of Porcine Hemagglutinating Encephalomyelitis Virus Spike Protein Interacts with the Neural Cell Adhesion Molecule
has PubMed Central identifier	PMC7179542
has PubMed identifier	25925196
schema:publication	Intervirology
resource representing a document's body	covid:ceaf1ce77440c43549de7a989c28376495b57e61#body_text
is schema:about of	named entity 'NCAM' named entity 'yeast two-hybrid' named entity 'NCAM' named entity 'protein' named entity 'infection' named entity 'NCAM' named entity 'Escherichia coli' named entity 'CLARIFIED' named entity 'FRAGMENT' named entity 'interaction' named entity 'screened' named entity 'pathology laboratory' named entity 'viruses' named entity 'RBD' named entity 'Genbank' named entity 'NCAM' named entity 'cell culture' named entity 'protein' named entity 'yeast' named entity 'The' named entity 'binding' named entity 'Three' named entity 'neural cell adhesion molecule' named entity 'The' named entity 'S protein' named entity 'fusion proteins' named entity 'amino acids' named entity 'pull-down' named entity 'NCAM' named entity 'NCAM' named entity 'RBD' named entity 'NCAM' named entity 'tropism' named entity 'fusion protein' named entity 'peripheral nerves' named entity 'yeast' named entity 'gene' named entity 'cellular receptor' named entity 'yeast' named entity 'electrophoresis' named entity 'GST protein' named entity 'plasmids' named entity 'central nervous' named entity 'chemotherapy' named entity 'SARS-CoV' named entity 'PCR' named entity 'protein' named entity 'RBD' named entity 'receptor' named entity 'genus' named entity 'gene products' named entity 'yeast two-hybrid' named entity 'cell-cell adhesion' named entity 'viral entry' named entity 'pET' named entity 'mmol/l' named entity 'Amersham' named entity 'amino acids' named entity 'NCAM' named entity 'NCAM' named entity 'protein' named entity 'infection' named entity 'cellular receptor' named entity 'RBD' named entity 'cellular receptor' named entity 'virus' named entity 'epitope' named entity 'coronaviruses' named entity 'diploid' named entity 'Y187'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software