About: Standard operating procedures for SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay

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About: Standard operating procedures for SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Standard operating procedures for SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay
Creator	Moore, David Beale, Rupert Swanton, Charles Grant, Paul Nastouli, Eleni Jiang, Ming Buck, Michael Poirier, Enzo Reis E Sousa, Caetano Walker, Philip Kassiotis, George Howell, Michael Barrell, Sam Byrne, Richard Caidan, Simon Canton, Johnathan Cardoso, Ana Crawford, Margaret Cubitt, Laura Frederico, Bruno Gamblin, Steve Gandhi, Sonia Goldstone, Robert Gulati, Kiran Hindmarsh, Steve Hubank, Michael Instrell, Rachael Lu, Wei-Ting Macrae, James Martini, Iana Miller, Davin Nicod, Jerome Nightingale, Luke O'reilly, Nicola Olsen, Jessica Oomatia, Amin Rideg, Anett Song, Ok-Ryul Strange, Amy Turajlic, Samra Wu, Mary
Source	MedRxiv
abstract	The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 40,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated standard operating procedure (SOP) for high-throughput SARS- CoV-2 detection by RT-LAMP in 25 minutes that is robust, reliable, repeatable, sensitive, specific, and inexpensive.
has issue date	2020-07-01(xsd:dateTime)
bibo:doi	10.1101/2020.06.29.20142430
has license	medrxiv
sha1sum (hex)	ce43ee44c048454733a77e25372dcd821829f8fe
schema:url	https://doi.org/10.1101/2020.06.29.20142430
resource representing a document's title	Standard operating procedures for SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay
resource representing a document's body	covid:ce43ee44c048454733a77e25372dcd821829f8fe#body_text
is schema:about of	named entity 'CLINICAL' named entity '40%' named entity 'SPECIFIC' named entity 'SARS-COV-2' named entity 'BIOMEDICAL RESEARCH' named entity 'RESEARCH INSTITUTE' named entity 'DIAGNOSTIC' named entity 'STANDARD OPERATING PROCEDURES' named entity 'THE CRICK' named entity 'standard operating procedure' named entity 'high-throughput' named entity 'RT-qPCR' named entity 'COVID' named entity 'Standard operating procedures' named entity 'SARS-CoV-2' named entity 'qPCR' named entity 'preprint' named entity 'DNA primers' named entity 'Fluorescent' named entity 'RT-qPCR' named entity 'peer review' named entity 'New England Biolabs' named entity 'SARS-CoV-2' named entity 'acceptance testing' named entity 'phosphoprotein' named entity 'SARS-CoV-2' named entity 'medRxiv' named entity 'Infection' named entity 'gene' named entity 'peer review' named entity 'medRxiv' named entity 'Real-Time PCR' named entity 'SARS-CoV-2' named entity 'medRxiv' named entity 'preprint' named entity 'peer review' named entity 'peer review' named entity 'peer review' named entity 'RNA extraction' named entity 'SARS-CoV-2' named entity 'preprint' named entity 'medRxiv' named entity 'medRxiv' named entity 'preprint' named entity 'Triton X-100'

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