About: Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum

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About: Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
Creator	Schares, Gereon Bandini, Luciana Cavalcante, Guacyara Gennari, Solange Neto, Aldo Nishi, Sandra Pena, Hilda
topic	covid:cdcb57ca4a03749e961145c638a2ba30aea8a1d8#this
Source	Elsevier; Medline; PMC
abstract	Abstract Neospora caninum is widely distributed in the world and this parasite is one of the major causes of abortion in cattle. Dogs and coyotes are definitive hosts of N. caninum and several species of domestic and wild animals are intermediate hosts. Dogs can become infected by the ingestion of tissues containing cysts and then excrete oocysts. It is not yet known whether sporulated oocysts are able to induce a patent infection in dogs, i.e. a shedding of N. caninum oocysts in feces. The objective of this study was to experimentally examine the infection of dogs by sporulated oocysts. The oocysts used in the experiment were obtained by feeding dogs with brain of buffaloes (Bubalus bubalis) positive for anti-N. caninum antibodies by indirect fluorescent antibody test (IFAT ≥200). Oocysts shed by these dogs were confirmed to be N. caninum by molecular methods and by bioassay in gerbils, and sporulated N. caninum oocysts were used for the oral infection of four dogs. The dogs were 8 weeks old and negative for antibodies to N. caninum and Toxoplasma gondii. Dogs 1 and 4 received an inoculum of 10,000 sporulated oocysts each; dog 2 an inoculum of 5000 sporulated oocysts and dog 3 received 1000 sporulated oocysts of N. caninum. The total feces excreted by these dogs were collected and examined daily for a period of 30 days. No oocysts were found in their feces. The dogs were monitored monthly for a 6-month period to observe a possible seroconversion and when this occurred the animals were eliminated from the experiment. Dogs 1 and 4 seroconverted 1 month after the infection with titer, in the IFAT, of 1600 and 800, respectively; the other two dogs presented no seroconvertion during the 6-month period. Dogs 1 and 2 were euthanized 180 days after infection and were examined for the detection of N. caninum in tissues (brain, muscle, lymph node, liver, lung, heart and bone marrow) by immunohistochemistry and PCR with negative results in both techniques. Bioassay in gerbils with brain of these dogs was also performed and again the results were negative. In conclusion, dogs infected with sporulated oocysts of N. caninum were not able to shed oocysts in feces. However, a higher dose of infection stimulated the production of antibodies against N. caninum in the dogs.
has issue date	2011-03-10(xsd:dateTime)
bibo:doi	10.1016/j.vetpar.2010.10.047
bibo:pmid	21094584
has license	els-covid
sha1sum (hex)	cdcb57ca4a03749e961145c638a2ba30aea8a1d8
schema:url	https://doi.org/10.1016/j.vetpar.2010.10.047
resource representing a document's title	Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
has PubMed Central identifier	PMC7116961
has PubMed identifier	21094584
schema:publication	Veterinary Parasitology
resource representing a document's body	covid:cdcb57ca4a03749e961145c638a2ba30aea8a1d8#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/cdcb57ca4a03749e961145c638a2ba30aea8a1d8
is schema:about of	named entity 'oocysts' named entity 'experimentally' named entity 'antibodies' named entity 'animals' named entity 'bone marrow' named entity 'indirect fluorescent antibody' named entity 'molecular' named entity 'immunohistochemistry' named entity 'received' named entity 'Bioassay' named entity 'infection' named entity '8 WEEKS' named entity 'BIOASSAY' named entity 'TOXOPLASMA GONDII' named entity 'BONE MARROW' named entity 'PRODUCTION' named entity 'IMMUNOHISTOCHEMISTRY' named entity 'SPECIES' named entity 'BRAIN' named entity 'GERBILS' named entity 'FOUND' named entity 'POSITIVE FOR' named entity 'CYSTS' named entity 'NEOSPORA CANINUM' named entity 'DOGS' named entity 'CONFIRMED' named entity 'INFECTED' named entity 'MUSCLE' named entity 'HEART' named entity 'OCCURRED' named entity 'FEEDING' covid:arg/cdcb57ca4a03749e961145c638a2ba30aea8a1d8 named entity 'NEGATIVE' named entity 'DISTRIBUTED' named entity 'EXAMINED' named entity 'RESULTS' named entity 'CONTAINING' named entity '1000' named entity 'NEGATIVE RESULTS' named entity 'ELIMINATED' named entity '1600' named entity 'DOMESTIC' named entity 'PERIOD' named entity 'OBJECTIVE' named entity 'MAJOR' named entity 'COLLECTED' named entity 'EXAMINED FOR' named entity 'HIGHER' named entity 'HOSTS' named entity 'DOG' named entity 'EUTHANIZED' named entity 'SEROCONVERSION' named entity 'CATTLE' named entity 'INOCULUM' named entity 'BUFFALOES' named entity 'MONITORED' named entity 'TECHNIQUES' named entity 'PARASITE' named entity 'FECES' named entity 'MOLECULAR' named entity '180' named entity 'PERFORMED' named entity 'EXPERIMENTAL INFECTION' named entity 'OOCYSTS' named entity 'PATENT' named entity 'USED' named entity 'DAYS' named entity 'WORLD' named entity 'RECEIVED' named entity 'METHODS' named entity 'WILD ANIMALS' named entity 'OBTAINED BY' named entity 'MONTHLY'

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