About: Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6

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About: Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6–positive Vesicles Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6–positive Vesicles
Creator	Klumperman, Judith Arvan, Peter Kuliawat, Regina Griffith, Janice Geuze, Hans
Source	Medline; PMC
abstract	The occurrence of clathrin-coated buds on immature granules (IGs) of the regulated secretory pathway suggests that specific transmembrane proteins are sorted into these buds through interaction with cytosolic adaptor proteins. By quantitative immunoelectron microscopy of rat endocrine pancreatic β cells and exocrine parotid and pancreatic cells, we show for the first time that the mannose 6–phosphate receptors (MPRs) for lysosomal enzyme sorting colocalize with the AP-1 adaptor in clathrin-coated buds on IGs. Furthermore, the concentrations of both MPR and AP-1 decline by ∼90% as the granules mature. Concomitantly, in exocrine secretory cells lysosomal proenzymes enter and then are sorted out of IGs, just as was previously observed in β cells (Kuliawat, R., J. Klumperman, T. Ludwig, and P. Arvan. 1997. J. Cell Biol. 137:595–608). The exit of MPRs in AP-1/clathrin-coated buds is selective, indicated by the fact that the membrane protein phogrin is not removed from maturing granules. We have also made the first observation of a soluble N-ethylmaleimide–sensitive factor attachment protein receptor, syntaxin 6, which has been implicated in clathrin-coated vesicle trafficking from the TGN to endosomes (Bock, J.B., J. Klumperman, S. Davanger, and R.H. Scheller. 1997. Mol. Biol. Cell. 8:1261–1271) that enters and then exits the regulated secretory pathway during granule maturation. Thus, we hypothesize that during secretory granule maturation, MPR–ligand complexes and syntaxin 6 are removed from IGs by AP-1/clathrin-coated vesicles, and then delivered to endosomes.
has issue date	1998-04-20(xsd:dateTime)
bibo:pmid	9548715
has license	cc-by-nc-sa
sha1sum (hex)	ccfba5ba6e11eff35a72dead3fac7f7daebda11d
resource representing a document's title	Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6–positive Vesicles
has PubMed Central identifier	PMC2148452
has PubMed identifier	9548715
schema:publication	J Cell Biol
resource representing a document's body	covid:ccfba5ba6e11eff35a72dead3fac7f7daebda11d#body_text
is schema:about of	named entity 'RECEPTORS' named entity 'AP-1' named entity 'SENSITIVE' named entity 'IMMATURE' named entity 'EXOCRINE' named entity 'TRANSMEMBRANE PROTEINS' named entity 'MANNOSE 6-PHOSPHATE' named entity 'ENTER' named entity 'INTERACTION' named entity 'SYNTAXIN 6' named entity 'SECRETORY GRANULE MATURATION' named entity 'INDICATED' named entity 'MATURE' named entity 'EXIT' named entity 'HAVE' named entity 'SPECIFIC' named entity 'DELIVERED' named entity 'BUDS' named entity '90%' named entity 'AP-1' named entity 'MOL' named entity 'OBSERVATION' named entity 'granule' named entity 'quantitative' named entity 'enters' named entity 'IGs' named entity 'delivered' named entity 'membrane protein' named entity 'MPRs' named entity 'exocrine' named entity 'PROTEIN ' named entity 'SYNTAXIN 6' covid:arg/ccfba5ba6e11eff35a72dead3fac7f7daebda11d named entity 'MATURATION' named entity 'OCCURRENCE' named entity 'OBSERVED' named entity 'SORTED' named entity 'SORTING' named entity 'CONCENTRATIONS' named entity 'THESE' named entity 'VESICLE TRAFFICKING' named entity 'LYSOSOMAL' named entity '608' named entity 'COATED' named entity 'PANCREATIC' named entity 'LYSOSOMAL ENZYME' named entity 'SORTED' named entity 'VESICLES' named entity 'ADAPTOR PROTEIN' named entity 'MANNOSE 6-PHOSPHATE' named entity 'COMPLEXES' named entity 'QUANTITATIVE' named entity 'ATTACHMENT' named entity 'CYTOSOLIC' named entity 'ADAPTOR' named entity 'ARVAN' named entity 'SECRETORY CELLS' named entity 'TIME' named entity '1271' named entity '80%' named entity 'CELL' named entity 'FACT' named entity 'REGULATED SECRETORY PATHWAY' named entity '1997' named entity 'COATED VESICLES' named entity 'IMMUNOELECTRON MICROSCOPY' named entity 'TGN' named entity 'PROTEIN RECEPTOR' named entity 'SELECTIVE' named entity 'ETHYLMALEIMIDE' named entity '137' named entity 'PREVIOUSLY' named entity 'GRANULES' named entity 'FACTOR' named entity 'MEMBRANE PROTEIN' named entity 'SOLUBLE' named entity 'POSITIVE' named entity 'PHOGRIN' named entity 'CELLS'

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