About: Rapid and Sensitive Recombinase Polymerase Amplification Combined With Lateral Flow Strip for Detecting African Swine Fever Virus

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About: Rapid and Sensitive Recombinase Polymerase Amplification Combined With Lateral Flow Strip for Detecting African Swine Fever Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid and Sensitive Recombinase Polymerase Amplification Combined With Lateral Flow Strip for Detecting African Swine Fever Virus
Creator	Li, Min Wang, Ying Chen, Teng Hu, Rongliang Li, Nan Mi, Lijuan Miao, Faming Wang, Lidong Wang, Shuchao Zhang, Fei Zhang, Jingyuan Zhang, Jinxia Zhang, Shoufeng Zhang, Yanyan Zhou, Xintao Zhang, Zhou Li, Y Wang, Wang Li, Zhang Bastos, Armanda
Source	Medline; PMC
abstract	African swine fever virus (ASFV), the etiological agent of African swine fever (ASF), a hemorrhagic fever of domestic pigs, has devastating consequences for the pig farming industry. More than 1,000,000 pigs have been slaughtered since 3 August 2018 in China. However, vaccines or drugs for ASF have yet to be developed. As such, a rapid test that can accurately detect ASFV on-site is important to the timely implementation of control measures. In this study, we developed a rapid test that combines recombinase polymerase amplification (RPA) of the ASFV p72 gene with lateral flow detection (LFD). Results showed that the sensitivity of recombinase polymerase amplification with lateral flow dipstick (RPA-LFD) for ASFV was 150 copies per reaction within 10 min at 38°C. The assay was highly specific to ASFV and had no cross-reactions with other porcine viruses, including classical swine fever virus (CSFV). A total of 145 field samples were examined using our method, and the agreement of the positive rate between RPA-LFD (10/145) and real-time PCR (10/145) was 100%. Overall, RPA-LFD provides a novel alternative for the simple, sensitive, and specific identification of ASFV and showed potential for on-site ASFV detection.
has issue date	2019-05-15(xsd:dateTime)
bibo:doi	10.3389/fmicb.2019.01004
bibo:pmid	31156571
has license	cc-by
sha1sum (hex)	cc563b84c16c4fe4fb1fbd3baa6ffd08dda70006
schema:url	https://doi.org/10.3389/fmicb.2019.01004
resource representing a document's title	Rapid and Sensitive Recombinase Polymerase Amplification Combined With Lateral Flow Strip for Detecting African Swine Fever Virus
has PubMed Central identifier	PMC6530510
has PubMed identifier	31156571
schema:publication	Front Microbiol
resource representing a document's body	covid:cc563b84c16c4fe4fb1fbd3baa6ffd08dda70006#body_text
is schema:about of	named entity 'MIN' named entity 'COMBINES' named entity 'TIMELY' named entity 'DETECTION' named entity 'SENSITIVE' named entity 'PIG' named entity 'REAL-TIME PCR ' named entity 'LATERAL' named entity 'RAPID' named entity 'IMPLEMENTATION' named entity 'MEASURES' named entity '80%' named entity 'DOMESTIC' named entity 'HAVE' named entity 'RECOMBINASE' named entity 'POSITIVE RATE' named entity 'ALTERNATIVE' named entity 'CLASSICAL SWINE FEVER VIRUS' named entity 'TEST' named entity 'NOVEL' named entity 'HIGHLY' named entity 'EXAMINED' named entity 'RAPID' named entity 'domestic pigs' named entity 'real-time PCR' named entity 'specific' named entity 'swine fever' named entity 'etiological agent' named entity 'However' named entity 'reaction' named entity 'With' named entity 'FLOW' covid:arg/cc563b84c16c4fe4fb1fbd3baa6ffd08dda70006 named entity 'AUGUST' named entity 'ASSAY' named entity 'CONTROL' named entity 'RESULTS' named entity 'DIPSTICK' named entity 'PIGS' named entity 'CROSS' named entity 'VIRUSES' named entity 'POLYMERASE' named entity 'IMPORTANT' named entity 'GENE' named entity 'FIELD' named entity 'USING' named entity 'LFD' named entity 'AGREEMENT' named entity 'VACCINES' named entity 'CITATION' named entity 'RECOMBINASE' named entity 'LATERAL' named entity 'AFRICAN SWINE FEVER VIRUS' named entity 'STRIP' named entity 'DETECTING' named entity 'SENSITIVE' named entity 'AMPLIFICATION' named entity 'ASFV P72' named entity 'INCLUDING' named entity 'COPIES' named entity 'MORE THAN' named entity 'REACTION'

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