About: Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein

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About: Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein
Creator	Han, Zongxi Kong, Xiangang Liu, Shengwang Liu, Xiaoli Shao, Yuhao Zhao, Fei Song, Yang
source	Elsevier; Medline; PMC
abstract	Abstract The nucleocapsid (N) protein of the infectious bronchitis virus (IBV) may play an essential role in the replication and translation of viral RNA. The N protein can also induce high titers of cross-reactive antibodies and cell-mediated immunity, which protects chickens from acute infection. In this study, we generated two monoclonal antibodies (mAbs), designated as 6D10 and 4F10, which were directed against the N protein of IBV using the whole viral particles as immunogens. Both of the mAbs do not cross react with Newcastle disease virus (NDV), infectious laryngotracheitis virus (ILTV) and subtype H9 avian influenza virus (AIV). After screening a phage display peptide library and peptide scanning, we identified two linear B-cell epitopes that were recognized by the mAbs 6D10 and 4F10, which corresponded to the amino acid sequences 242FGPRTK247 and 195DLIARAAKI203, respectively, in the IBV N protein. Alignments of amino acid sequences from a large number of IBV isolates indicated that the two epitopes, especially 242FGPRTK247, were well conserved among IBV strains. This conclusion was further confirmed by the relationships of 18 heterologous sequences to the 2 mAbs. The novel mAbs and the epitopes identified will be useful for developing diagnostic assays for IBV infections.
has issue date	2013-01-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2012.10.028
bibo:pmid	23123213
has license	els-covid
sha1sum (hex)	c7ce8964ad1b277178324ec79b4b70c9714baa6f
schema:url	https://doi.org/10.1016/j.virusres.2012.10.028
resource representing a document's title	Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein
has PubMed Central identifier	PMC7114416
has PubMed identifier	23123213
schema:publication	Virus Research
resource representing a document's body	covid:c7ce8964ad1b277178324ec79b4b70c9714baa6f#body_text
is schema:about of	named entity 'EPITOPE MAPPING' named entity 'AVIAN INFECTIOUS BRONCHITIS' named entity 'CORONAVIRUS' named entity 'CONSERVATION' named entity 'ESSENTIAL' named entity 'ACUTE INFECTION' named entity 'ISOLATES' named entity 'NOVEL' named entity 'AVIAN INFLUENZA VIRUS' named entity 'PROTEIN CAN' named entity 'CONFIRMED BY' named entity 'INDUCE' named entity 'IDENTIFIED' named entity 'FINE' named entity 'LARGE' named entity 'NUCLEOCAPSID' named entity 'INFECTIOUS LARYNGOTRACHEITIS VIRUS' named entity 'INDICATED' named entity 'NUCLEOCAPSID PROTEIN' named entity 'EPITOPES' named entity 'LINEAR' named entity 'NOVEL' named entity 'LEVEL' named entity 'B-CELL' named entity 'PROTEIN ' named entity 'MONOCLONAL ANTIBODIES' named entity 'AMINO ACID SEQUENCES' named entity '242' named entity 'DIRECTED' named entity 'SEQUENCES' named entity 'DEVELOPING' named entity 'REPLICATION' named entity '203' named entity 'DIAGNOSTIC' named entity 'N PROTEIN' named entity 'CONCLUSION' named entity 'IMMUNOGENS' named entity 'CELL-MEDIATED IMMUNITY' named entity 'SCANNING' named entity 'PHAGE DISPLAY PEPTIDE LIBRARY' named entity 'GENERATED' named entity 'CROSS' named entity 'CHICKENS' named entity 'RELATIONSHIPS' named entity 'ANALYSIS' named entity 'NUMBER OF' named entity 'VIRAL PARTICLES' named entity 'INFECTIONS' named entity '195' named entity '4F10' named entity 'NEWCASTLE DISEASE VIRUS' named entity 'B-CELL' named entity 'SCREENING' named entity 'PEPTIDE' named entity 'USING' named entity 'HIGH' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'SUBTYPE' named entity 'ANTIBODIES' named entity 'DO NOT' named entity 'CROSS-REACTIVE' named entity 'LINEAR' named entity 'PLAY' named entity 'STRAINS' named entity 'CONSERVED' named entity 'REACT' named entity 'HETEROLOGOUS' named entity 'TRANSLATION' named entity 'ROLE' named entity 'STUDY' named entity 'EPITOPES' named entity 'VIRAL RNA' named entity 'USEFUL' named entity 'DESIGNATED' covid:arg/c7ce8964ad1b277178324ec79b4b70c9714baa6f

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