About: Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip

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About: Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
Creator	Al Amin, Md Amin, Al Bcd, Rahman Chowdhury, Muhammad Desa, Desa, Mohd Hussain, Md Hussain, Mohd Msa, Razimi Nasir, Mohd Nasri, Md Nasri, Muhammad Rahman, Mahfujur Rahman, Mohd Razimi, Razimi, Ahmad Shahril, Ahmad Shahril, Mohd Zaira, Zaman Zaman Chowdhury, Zaira Zz, Chowdhury
Source	Elsevier; Medline; PMC
abstract	Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to screen feline meat in commercial meat products using lab-on-a-chip. The SP-PCR assay proved its specificity theoretically and experimentally while testing with different common animal, aquatic and plant species of DNA. The feline specific (69 bp, 43- and 26-bp) characteristic molecular DNA pattern was observed by SP-PCR and RFLP analysis. For assay performance, it was tested in three different types of commercial dummy meat products such as frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The highest sensitivity of the assay using lab-on-a-chip was as low as 0.1 pg or 0.01% (w/w) in commercial dummy meat products. We have also applied this assay to screen three important commercial meat products of six different brand from six supermarket chains located at three different states of Malaysia. Thus total 378 samples were tested to validate the specificity, sensitivity, stability of the assay and utilization of it for commercial meat product screening.
has issue date	2020-06-09(xsd:dateTime)
bibo:doi	10.1016/j.jfca.2020.103565
bibo:pmid	32546895
has license	no-cc
sha1sum (hex)	c56426d5c8654cfa7c7455279e9d7ca2ab8524eb
schema:url	https://doi.org/10.1016/j.jfca.2020.103565
resource representing a document's title	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
has PubMed Central identifier	PMC7282760
has PubMed identifier	32546895
schema:publication	J Food Compost Anal
resource representing a document's body	covid:c56426d5c8654cfa7c7455279e9d7ca2ab8524eb#body_text
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is wasQuotedFrom of	covid:arg/c56426d5c8654cfa7c7455279e9d7ca2ab8524eb/0 covid:arg/c56426d5c8654cfa7c7455279e9d7ca2ab8524eb/1

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