About: SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms

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About: SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms
Creator	Resende, Paola Breuer, Judith Siqueira, Marilda Caetano, Braulia Harris, Kathryn Matos, Aline Miranda, Milene Garcia, Cristiana Xavier, Joilson Williams, Rachel Roy, Sunando Abreu, André Appolinario, Luciana Couto Motta, Fernando Fabri, Allison Orgeswalska, Maria
Source	BioRxiv
abstract	Genomic surveillance has become a useful tool for better understanding virus pathogenicity, origin and spread. Obtaining accurately assembled, complete viral genomes directly from clinical samples is still a challenging. Here, we describe three protocols using a unique primer set designed to recover long reads of SARS-CoV-2 directly from total RNA extracted from clinical samples. This protocol is useful, accessible and adaptable to laboratories with varying resources and access to distinct sequencing methods: Nanopore, Illumina and/or Sanger.
has issue date	2020-05-01(xsd:dateTime)
bibo:doi	10.1101/2020.04.30.069039
has license	biorxiv
sha1sum (hex)	c5196e2d31e684fe21f2a54e17aead2f4ff237eb
schema:url	https://doi.org/10.1101/2020.04.30.069039
resource representing a document's title	SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms
schema:publication	bioRxiv
resource representing a document's body	covid:c5196e2d31e684fe21f2a54e17aead2f4ff237eb#body_text
is schema:about of	named entity 'viral genomes' named entity 'tool' named entity 'LABORATORIES' named entity 'USEFUL' named entity 'TOTAL RNA' named entity 'READS' named entity 'PRIMER' named entity 'BETTER' named entity 'amplicons' named entity 'Illumina' named entity 'clinical samples' named entity 'GISAID' named entity 'subtyping' named entity 'CoV' named entity 'GISAID' named entity 'SARS-CoV-2' named entity 'genome sequences' named entity 'Agilent' named entity 'Multiplex PCR' named entity 'genome' named entity 'San Diego' named entity 'Illumina' named entity 'clinical samples' named entity 'fmol' named entity 'multiplex PCR' named entity 'GISAID' named entity 'multiplex PCR' named entity 'amplicons' named entity 'Oxford Nanopore' named entity 'Nextera' named entity 'high fidelity' named entity 'Sanger' named entity 'Wuhan' named entity 'pathogenicity' named entity 'SARS-CoV-2' named entity 'virus transmission' named entity 'viral genomes' named entity 'consensus sequence' named entity 'RNA' named entity 'Nanopore sequencing' named entity 'genus' named entity 'nucleic acid' named entity 'genome' named entity 'SARS-CoV-2' named entity 'amplicon' named entity 'Sanger' named entity 'pathogenicity' named entity 'genomes' named entity 'SARS-CoV-2' named entity 'COVID' named entity 'Pathogen Genomics' named entity 'nucleotides' named entity 'primer' named entity 'higher throughput' named entity '1, 2' named entity 'SARS-CoV-2' named entity 'viral genome' named entity 'SARS-CoV-2 pandemic' named entity 'genome sequences' named entity 'base pairs' named entity 'genome' named entity 'Invitrogen' named entity 'Illumina platform' named entity 'SARS-CoV-2' named entity 'Zika' named entity 'primers' named entity 'WHO' named entity 'Chikungunya' named entity 'Medaka'

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